EMDB EMD-10180

Single particle reconstruction
3.3Å resolution

mt-SSU assemblosome of Trypanosoma brucei

Map released:
Overview of EMD-10180
Source organism: Trypanosoma brucei brucei [5702]
Fitted atomic model: 6sgb
Related EM entries by publication: EMD-10175, EMD-10176, EMD-10177, EMD-10178, EMD-10179
Primary publication:
Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.
Saurer M, Ramrath DJF, Niemann M, Calderaro S, Prange C, Mattei S, Scaiola A, Leitner A, Bieri P, Horn EK, Leibundgut M, Boehringer D, Schneider A, Ban N
Science 365 1144-1149 (2019)
PMID: 31515389

Function and Biology Details

Sample name: mt-SSU assemblosome
Ligands: MAGNESIUM ION, PHOSPHATE ION, S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE, ZINC ION, GUANOSINE-5'-TRIPHOSPHATE, S-ADENOSYL-L-HOMOCYSTEINE, water
Nucleic acid: 9S rRNA
Proteins: mt-SSU assemblosome, uS5m, bS6m, uS8m, uS11m, uS15m, bS16m, uS17m, bS18m, mS22, mS23, mS26, mS34, mS38, mS41, mS51 (KRIPP1), mS56, mS59, mS62 (KRIPP14), mS63 (KRIPP16), mS65, mS68, mS73, mt-SAF2 (KRIPP2), mt-SAF3, mt-SAF5, mt-SAF6, mt-SAF7 (KRIPP10), mt-SAF8, mt-SAF9, mt-SAF10, mt-SAF11, mt-SAF13, mt-SAF18, mt-SAF21, mt-SAF22 (KRIPP17), mt-SAF23, mt-SAF24, mt-SAF26, mt-SAF27 (KRIPP11), mt-SAF28, mt-SAF29, mt-SAF30, mt-SAF31, mt-SAF32, mt-SAF33, UNK-A, UNK-B, UNK-k, UNK-C, UNK-D, UNK-M, UNK-Q, UNK-f, UNK-E, UNK-P, UNK-F, UNK-m, UNK-G, UNK-H, UNK-I, UNK-N, UNK-J, UNK-K, UNK-L, UNK-O, UNK-Y, mS3m, uS9m, uS10m, uS14m, uS19m, mS29, mS33, mS35, mS49, mS50, mS52, mS53, mS54, mS55 (KRIPP8), mS57, mS58, mS67, mS69, mS70, mS71, mS72, mt-SAF1 (RSM22), mt-SAF4, mt-SAF12 (KRIPP18), mt-SAF14, mt-SAF15, mt-SAF16, mt-SAF17, mt-SAF19, mt-SAF20, mt-SAF25, mt-SAF34, UNK-a, UNK-b, UNK-c, UNK-d, UNK-e, UNK-g, UNK-h, UNK-i, UNK-j, UNK-l, UNK-x

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)