EMDB EMD-10177

Single particle reconstruction
3.1Å resolution

Body domain of the mt-SSU assemblosome from Trypanosoma brucei

Map released:
Last modified:
Overview of EMD-10177
Source organism: Trypanosoma brucei brucei [5702]
Fitted atomic model: 6sga
Related EM entries by publication: EMD-10175, EMD-10176, EMD-10178, EMD-10179, EMD-10180
Primary publication:
Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.
Saurer M, Ramrath DJF, Niemann M, Calderaro S, Prange C, Mattei S, Scaiola A, Leitner A, Bieri P, Horn EK, Leibundgut M, Boehringer D, Schneider A, Ban N
Science 365 1144-1149 (2019)
PMID: 31515389

Function and Biology Details

Sample name: Body domain of the mt-SSU assemblosome
Ligands: MAGNESIUM ION, PHOSPHATE ION, S-(2-{[N-(2-HYDROXY-4-{[HYDROXY(OXIDO)PHOSPHINO]OXY}-3,3-DIMETHYLBUTANOYL)-BETA-ALANYL]AMINO}ETHYL) DECANETHIOATE, ZINC ION
Nucleic acid: 9S rRNA
Proteins: Body domain of the mt-SSU assemblosome, uS5m, bS6m, uS8m, uS11m, uS15m, bS16m, uS17m, bS18m, mS22, mS23, mS26, mS34, mS38, mS41, mS51 (KRIPP1), mS56, mS59, mS62 (KRIPP14), mS63 (KRIPP16), mS65, mS68, mS73, mt-SAF2 (KRIPP2), mt-SAF3, mt-SAF5, mt-SAF6, mt-SAF7 (KRIPP10), mt-SAF8, mt-SAF9, mt-SAF10, mt-SAF11, mt-SAF13, mt-SAF18, mt-SAF21, mt-SAF22 (KRIPP17), mt-SAF23, mt-SAF24, mt-SAF26, mt-SAF27, mt-SAF28, mt-SAF29, mt-SAF30, mt-SAF31, mt-SAF32, mt-SAF33, UNK-A, UNK-B, UNK-C, UNK-D, UNK-M, UNK-Q, UNK-E, UNK-P, UNK-F, UNK-G, UNK-H, UNK-I, UNK-M, UNK-J, UNK-L, UNK-O, UNK-U, UNK-I, mt-SAF1 (RSM22), mt-SAF14

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)