EMDB EMD-10175

Single particle reconstruction
3.1Å resolution

Head domain of the mt-SSU assemblosome from Trypanosoma brucei

Map released:
Last modified:
Overview of EMD-10175
Source organism: Trypanosoma brucei brucei [5702]
Fitted atomic model: 6sg9
Related EM entries by publication: EMD-10176, EMD-10177, EMD-10178, EMD-10179, EMD-10180
Primary publication:
Mitoribosomal small subunit biogenesis in trypanosomes involves an extensive assembly machinery.
Saurer M, Ramrath DJF, Niemann M, Calderaro S, Prange C, Mattei S, Scaiola A, Leitner A, Bieri P, Horn EK, Leibundgut M, Boehringer D, Schneider A, Ban N
Science 365 1144-1149 (2019)
PMID: 31515389

Function and Biology Details

Sample name: Cryo-EM structure of the head domain of the mt-SSU assemblosome from Trypanosoma brucei
Ligands: GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION, ZINC ION, S-ADENOSYL-L-HOMOCYSTEINE, water
Nucleic acid: 9S rRNA
Proteins: Cryo-EM structure of the head domain of the mt-SSU assemblosome from Trypanosoma brucei, uS5m, uS11m, mS38, mt-SAF18, mt-SAF28, mt-SAF30, uS3m, uS9m, uS10m, uS14m, uS19m, mS29, mS33, mS35, mS49, mS50, mS52, mS53, mS54, mS55 (KRIPP8), mS57, mS58, mS67, mS69, mS70, mS71, mS72, mt-SAF1 (RSM22), mt-SAF4, mt-SAF12 (KRIPP18), mt-SAF14, mt-SAF15, mt-SAF16, mt-SAF17, mt-SAF19, mt-SAF20, mt-SAF25, mt-SAF34, UNK-a, UNK-b, UNK-c, UNK-d, UNK-e, UNK-f, UNK-g, UNK-h, UNK-i, UNK-j, UNK-k, UNK-l, UNK-m, UNK-x

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)