EMDB EMD-10073

Single particle reconstruction
3.1Å resolution

Ribosome nascent chain in complex with SecA

Map released:
Overview of EMD-10073
Source organism: Escherichia coli [562]
Fitted atomic model: 6s0k
Related EM entry by publication: EMD-10074
Primary publication:
The molecular mechanism of cotranslational membrane protein recognition and targeting by SecA.
Wang S, Jomaa A, Jaskolowski M, Yang CI, Ban N, Shan SO
Nat.Struct.Mol.Biol. - (2019)
PMID: 31570874

Function and Biology Details

Sample name: ribosome nascent chain in complex with SecA
Ligands: MAGNESIUM ION, ZINC ION
Nucleic acids: tRNA-CCA, 23S ribosomal RNA, 5S ribosomal RNA
Proteins: ribosome nascent chain in complex with SecA, ribosome, SecA, nascent chain, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L10, 50S ribosomal protein L11, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L24, 50S ribosomal protein L25, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, Protein translocase subunit SecA, Cytoskeleton protein RodZ

Experimental Information Details

Resolution: 3.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)