EMDB EMD-10054

Single particle reconstruction
7.1Å resolution

Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state C

Map released:
Overview of EMD-10054
Source organism: Chaetomium thermophilum [209285]
Fitted atomic model: 6rxx
Related EM entries by publication: EMD-10051, EMD-10052, EMD-10053, EMD-10055, EMD-10056
Primary publication:
Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.
Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E
Mol.Cell - (2019)
PMID: 31378463

Function and Biology Details

Sample name: 90S pre-ribosome, state C
Ligand: ZINC ION
Nucleic acids: 35S ribosomal RNA, U3 snoRNA
Proteins: 90S pre-ribosome, state C, Periodic tryptophan protein 2-like protein, Utp3, Utp6, Utp7, U3 small nucleolar RNA-associated protein 11, Utp12, Utp13, Utp14, Utp18, Putative U3 snoRNP protein, Utp24, Imp3, Putative U3 small nucleolar ribonucleoprotein, Putative U3 small nucleolar ribonucleoprotein protein, Sof1, Emg1, KRR1 small subunit processome component, Pre-rRNA-processing protein PNO1, 40S ribosomal protein S1, 40S ribosomal protein s5-like protein, 40S ribosomal protein S7, 40S ribosomal protein s9-like protein, 40S ribosomal protein S13-like protein, 40S ribosomal protein S14-like protein, 40S ribosomal protein S16-like protein, 40S ribosomal protein S22-like protein, 40S ribosomal protein s23-like protein, 40S ribosomal protein S28-like protein, Ribosomal protein s27-like protein, Faf1, U3 small nucleolar RNA-associated protein 22, Rrp7, Putative ribosomal protein, RNA 3'-terminal phosphate cyclase-like protein, Bms1, Kre33, 40S ribosomal protein S4, 40S ribosomal protein S6, 40S ribosomal protein S8, 40S ribosomal protein S11-like protein, 40S ribosomal protein S24, Ribosome biogenesis protein ENP2, Utp20, Bfr2, Utp2, Utp4, UTP10, Utp15, Utp17, Enp1, Utp5, Utp16, Utp8, Noc4, Nop1, Putative nucleolar protein, Nop58, Snu13, Rrp9, Fcf2, 40S ribosomal protein S19-like protein

Experimental Information Details

Resolution: 7.1Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Detector: FEI FALCON II (4k x 4k)