EMDB EMD-10053

Single particle reconstruction
4.0Å resolution

Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state B2

Map released:
Last modified:
Overview of EMD-10053
Fitted atomic model: 6rxv
Related EM entries by publication: EMD-10051, EMD-10052, EMD-10054, EMD-10055, EMD-10056
Primary publication:
Thermophile 90S Pre-ribosome Structures Reveal the Reverse Order of Co-transcriptional 18S rRNA Subdomain Integration.
Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E
Mol.Cell 75 1256-1269.e7 (2019)
PMID: 31378463

Function and Biology Details

Sample name: 90S pre-ribosome, state B2
Ligands: ZINC ION, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Nucleic acids: 35S ribosomal RNA, U3 snoRNA
Proteins: 90S pre-ribosome, state B2, Periodic tryptophan protein 2-like protein, Utp2, Utp3, Utp4, Utp6, Utp7, UTP10, U3 small nucleolar RNA-associated protein 11, Utp12, Utp13, Utp14, Utp15, Utp17, Utp18, Utp21, Utp24, Utp30, Nop1, Nop56, Nop58, Snu13, Rrp9, RNA 3'-terminal phosphate cyclase-like protein, Bms1, Imp3, Imp4, Mpp10, Sof1, Emg1, KRR1 small subunit processome component, Pre-rRNA-processing protein PNO1, Kre33, Fcf2, 40S ribosomal protein S1, 40S ribosomal protein S4, 40S ribosomal protein s5-like protein, 40S ribosomal protein S6, 40S ribosomal protein S7, 40S ribosomal protein S8, 40S ribosomal protein s9-like protein, 40S ribosomal protein S13-like protein, 40S ribosomal protein S14-like protein, 40S ribosomal protein S16-like protein, 40S ribosomal protein S11-like protein, 40S ribosomal protein S22-like protein, 40S ribosomal protein s23-like protein, 40S ribosomal protein S24, 40S ribosomal protein S28-like protein, Faf1, U3 small nucleolar RNA-associated protein 22, Rrp7, Ribosome biogenesis protein ENP2, Utp20, Utp8, Utp5, Noc4, Rps18, Enp1, U3 small nucleolar ribonucleoprotein protein lcp5-like protein, Bfr2, Utp16

Experimental Information Details

Resolution: 4.0Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)