EMDB EMD-0953

Single particle reconstruction
4.9Å resolution

Cryo-EM structure of 90S small subunit preribosomes in transition states (State E)

Map released:
Overview of EMD-0953
Source organism: Baker's yeast [559292]
Fitted atomic model: 6lqt
Primary publication:
Cryo-EM structure of 90S small ribosomal subunit precursors in transition states
Du Y, An W, Zhu X, Sun Q, Qi J, Ye K
Science - (2020)

Function and Biology Details

Sample name: 90S pre-ribosome (Dhr1-depleted, state E)
Ligands: ZINC ION, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Nucleic acids: U3 snoRNA, 5' ETS, 18S pre-rRNA
Proteins: 90S pre-ribosome (Dhr1-depleted, state E), 40S ribosomal protein S1-A, 40S ribosomal protein S4-A, 40S ribosomal protein S5, 40S ribosomal protein S6-A, 40S ribosomal protein S7-A, 40S ribosomal protein S8-A, 40S ribosomal protein S9-A, 40S ribosomal protein S11-A, 40S ribosomal protein S13, 40S ribosomal protein S14-A, 40S ribosomal protein S16-A, 40S ribosomal protein S22-B, 40S ribosomal protein S23-A, 40S ribosomal protein S24-A, 40S ribosomal protein S27-A, 40S ribosomal protein S28-A, rRNA 2'-O-methyltransferase fibrillarin, Nucleolar protein 56, Nucleolar protein 58, Ribosomal RNA-processing protein 9, 13 kDa ribonucleoprotein-associated protein, U3 small nucleolar RNA-associated protein 4, U3 small nucleolar RNA-associated protein 5, U3 small nucleolar RNA-associated protein 9, U3 small nucleolar RNA-associated protein 10, U3 small nucleolar RNA-associated protein 15, NET1-associated nuclear protein 1, Periodic tryptophan protein 2, U3 small nucleolar RNA-associated protein 12, U3 small nucleolar RNA-associated protein 13, U3 small nucleolar RNA-associated protein 18, U3 small nucleolar RNA-associated protein 21, U3 small nucleolar RNA-associated protein 6, U3 small nucleolar RNA-associated protein 7, U3 small nucleolar RNA-associated protein 11, U3 small nucleolar RNA-associated protein MPP10, U3 small nucleolar ribonucleoprotein protein IMP3, U3 small nucleolar ribonucleoprotein protein IMP4, Something about silencing protein 10, Protein SOF1, rRNA-processing protein FCF2, rRNA-processing protein FCF1, rRNA biogenesis protein RRP5, U3 small nucleolar RNA-associated protein 22, Ribosomal RNA-processing protein 7, Ribosomal RNA small subunit methyltransferase NEP1, Ribosome biogenesis protein BMS1, RNA 3'-terminal phosphate cyclase-like protein, Nucleolar complex protein 14, U3 small nucleolar RNA-associated protein 20, U3 small nucleolar RNA-associated protein 14, Pno1, Unassigned helices

Experimental Information Details

Resolution: 4.9Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)