EMDB EMD-0952

Single particle reconstruction
3.8Å resolution

Cryo-EM structure of 90S small subunit preribosomes in transition states (State D)

Map released:
Overview of EMD-0952
Source organism: Baker's yeast [559292]
Fitted atomic model: 6lqs
Primary publication:
Cryo-EM structure of 90S small ribosomal subunit precursors in transition states
Du Y, An W, Zhu X, Sun Q, Qi J, Ye K
Science - (2020)

Function and Biology Details

Sample name: 90S pre-ribosome (Dhr1-depleted, state D)
Ligands: ZINC ION, GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Nucleic acids: U3 snoRNA, 5' ETS, 18S pre-rRNA
Proteins: 90S pre-ribosome (Dhr1-depleted, state D), 40S ribosomal protein S1-A, 40S ribosomal protein S4-A, 40S ribosomal protein S5, 40S ribosomal protein S6-A, 40S ribosomal protein S7-A, 40S ribosomal protein S8-A, 40S ribosomal protein S9-A, 40S ribosomal protein S11-A, 40S ribosomal protein S13, 40S ribosomal protein S14-A, 40S ribosomal protein S16-A, 40S ribosomal protein S22-B, 40S ribosomal protein S23-A, 40S ribosomal protein S24-A, 40S ribosomal protein S27-A, 40S ribosomal protein S28-A, rRNA 2'-O-methyltransferase fibrillarin, Nucleolar protein 56, Nucleolar protein 58, Ribosomal RNA-processing protein 9, 13 kDa ribonucleoprotein-associated protein, U3 small nucleolar RNA-associated protein 4, U3 small nucleolar RNA-associated protein 5, U3 small nucleolar RNA-associated protein 8, U3 small nucleolar RNA-associated protein 9, U3 small nucleolar RNA-associated protein 10, U3 small nucleolar RNA-associated protein 15, NET1-associated nuclear protein 1, Periodic tryptophan protein 2, U3 small nucleolar RNA-associated protein 12, U3 small nucleolar RNA-associated protein 13, U3 small nucleolar RNA-associated protein 18, U3 small nucleolar RNA-associated protein 21, U3 small nucleolar RNA-associated protein 6, Bud site selection protein 21, U3 small nucleolar RNA-associated protein 7, U3 small nucleolar RNA-associated protein 11, U3 small nucleolar RNA-associated protein MPP10, U3 small nucleolar ribonucleoprotein protein IMP3, U3 small nucleolar ribonucleoprotein protein IMP4, Something about silencing protein 10, Protein SOF1, rRNA-processing protein FCF2, rRNA-processing protein FCF1, rRNA biogenesis protein RRP5, U3 small nucleolar RNA-associated protein 22, Ribosomal RNA-processing protein 7, Ribosomal RNA small subunit methyltransferase NEP1, Ribosome biogenesis protein BMS1, RNA 3'-terminal phosphate cyclase-like protein, Nucleolar complex protein 14, Nucleolar complex protein 4, U3 small nucleolar RNA-associated protein 20, U3 small nucleolar RNA-associated protein 14, Essential nuclear protein 1, Pno1, Regulator of rDNA transcription protein 14, Unassigned helices 1, Unassigned helices 2, Exosome complex component RRP45, Exosome complex component SKI6, Exosome complex component RRP43, Exosome complex component RRP46, Exosome complex component RRP42, Exosome complex component MTR3, Exosome complex component RRP40, Exosome complex component RRP4, Exosome complex component CSL4, Exosome complex exonuclease DIS3, Exosome complex exonuclease RRP6, Exosome complex protein LRP1, ATP-dependent RNA helicase DOB1, M-phase phosphoprotein 6 homolog

Experimental Information Details

Resolution: 3.8Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)