EMDB EMD-0835

Single particle reconstruction
2.4Å resolution

Structure of the PSI-FCPI supercomplex from diatom

Map released:
Overview of EMD-0835
Source organism: Chaetoceros gracilis [184592]
Fitted atomic model: 6l4u
Related EM entry by publication: EMD-0834
Primary publication:
Structure of photosystem I-light-harvesting supercomplex from a red-lineage diatom
Nagao R, Kato K, Ifuku K, Suzuki T, Kumazawa M, Uchiyama I, Kashino Y, Dohmae N, Akimoto S, Shen JR, Miyazaki N, Akita F
Nat Commun - (2020)

Function and Biology Details

Sample name: PSI-FCPI
Ligands: CHLOROPHYLL A ISOMER, CHLOROPHYLL A, PHYLLOQUINONE, IRON/SULFUR CLUSTER, BETA-CAROTENE, 1,2-DIPALMITOYL-PHOSPHATIDYL-GLYCEROLE, DODECYL-BETA-D-MALTOSIDE, 1,2-DISTEAROYL-MONOGALACTOSYL-DIGLYCERIDE, (3S,3'S,5R,5'R,6S,6'R,8'R)-3,5'-dihydroxy-8-oxo-6',7'-didehydro-5,5',6,6',7,8-hexahydro-5,6-epoxy-beta,beta-caroten-3'-yl acetate, Chlorophyll c1, (3S,3'R,5R,6S,7cis)-7',8'-didehydro-5,6-dihydro-5,6-epoxy-beta,beta-carotene-3,3'-diol, water
Proteins: PSI-FCPI, Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I P700 chlorophyll a apoprotein A2, Photosystem I iron-sulfur center, Photosystem I reaction center subunit II, Photosystem I reaction center subunit IV, Photosystem I reaction center subunit III, Photosystem I reaction center subunit VIII, Photosystem I reaction center subunit IX, Photosystem I reaction center subunit XI, Photosystem I reaction center subunit XII, Unknown protein 1, Photosystem I reaction center subunit Psa28, Fucoxanthin chlorophyll a/c-binding protein Lhcr15, Fucoxanthin chlorophyll a/c-binding protein Lhcr8, Fucoxanthin chlorophyll a/c-binding protein Lhcr2, Fucoxanthin chlorophyll a/c-binding protein Lhcr9, Fucoxanthin chlorophyll a/c-binding protein Lhcr11, Fucoxanthin chlorophyll a/c-binding protein Lhcr12, Fucoxanthin chlorophyll a/c-binding protein Lhcr10, Fucoxanthin chlorophyll a/c-binding protein Lhcr4, Fucoxanthin chlorophyll a/c-binding protein Lhcf6, Fucoxanthin chlorophyll a/c-binding protein Lhcr3, Fucoxanthin chlorophyll a/c-binding protein Lhcq13, Fucoxanthin chlorophyll a/c-binding protein Lhcq3, Fucoxanthin chlorophyll a/c-binding protein Lhcq11, Fucoxanthin chlorophyll a/c-binding protein Lhcq10, Fucoxanthin chlorophyll a/c-binding protein Lhcq8, Fucoxanthin chlorophyll a/c-binding protein Lhcq5

Experimental Information Details

Resolution: 2.4Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)