EMDB EMD-0744

Single particle reconstruction
2.9Å resolution

Cryo-EM structure of a class A GPCR with G protein complex

Map released:
Overview of EMD-0744
Source organism: Homo sapiens [9606]
Fitted atomic model: 6kpf
Related EM entry by publication: EMD-0745
Primary publication:
Activation and Signaling Mechanism Revealed by Cannabinoid Receptor-GiComplex Structures.
Hua T, Li X, Wu L, Iliopoulos-Tsoutsouvas C, Wang Y, Wu M, Shen L, Johnston CA, Nikas SP, Song F, Song X, Yuan S, Sun Q, Wu Y, Jiang S, Grim TW, Benchama O, Stahl EL, Zvonok N, Zhao S, Bohn LM, Makriyannis A, Liu ZJ
Cell - (2020)
PMID: 32004463

Function and Biology Details

Sample name: GPCR-G protein complex
Ligand: (6~{a}~{R},9~{R},10~{a}~{R})-9-(hydroxymethyl)-3-(8-isothiocyanato-2-methyl-octan-2-yl)-6,6-dimethyl-6~{a},7,8,9,10,10~{a}-hexahydrobenzo[c]chromen-1-ol
Proteins: GPCR-G protein complex, Guanine nucleotide-binding protein G(i) subunit alpha-1, Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-1, Guanine nucleotide-binding protein G(I)/G(S)/G(O) subunit gamma-2, Cannabinoid receptor 2, scFv16

Experimental Information Details

Resolution: 2.9Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)