EMDB EMD-0742

Single particle reconstruction
2.75Å resolution

277 K cryoEM structure of Sso-KARI in complex with Mg2+, NADH and CPD

Map released:
Overview of EMD-0742
Source organism: Saccharolobus solfataricus [2287]
Fitted atomic model: 6kpa
Primary publication:
Temperature-Resolved Cryo-EM Uncovers Structural Bases of Temperature-Dependent Enzyme Functions.
Chen CY, Chang YC, Lin BL, Huang CH, Tsai MD
J.Am.Chem.Soc. 141 19983-19987 (2019)
PMID: 31829582

Function and Biology Details

Sample name: KARI-Mg2+/NADH/CPD complex
Ligands: MAGNESIUM ION, 1,4-DIHYDRONICOTINAMIDE ADENINE DINUCLEOTIDE, cyclopropane-1,1-dicarboxylic acid
Proteins: KARI-Mg2+/NADH/CPD complex, Ketol-acid reductoisomerase

Experimental Information Details

Resolution: 2.75Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)