EMDB EMD-0693

Single particle reconstruction
4.0Å resolution

Cryo-EM structure of human MLL3-ubNCP complex (4.0 angstrom)

Map released:
Last modified:
Overview of EMD-0693
Source organisms:
Fitted atomic model: 6kiw
Related EM entries by publication: EMD-0694, EMD-0695, EMD-9998, EMD-9999
Primary publication:
Structural basis of nucleosome recognition and modification by MLL methyltransferases.
Xue H, Yao T, Cao M, Zhu G, Li Y, Yuan G, Chen Y, Lei M, Huang J
Nature 573 445-449 (2019)
PMID: 31485071

Function and Biology Details

Sample name: Human MLL3 complex associated with an H2B-monoubiquitinated nucleosome (4.0 angstrom)
Ligand: ZINC ION
Nucleic acids: DNA (144-MER), DNA (145-MER)
Proteins: Human MLL3 complex associated with an H2B-monoubiquitinated nucleosome (4.0 angstrom), Human MLL3, H2B-monoubiquitinated nucleosome, Histone H3, Histone H4, Histone H2A, Histone H2B 1.1, Histone-lysine N-methyltransferase 2C, Retinoblastoma-binding protein 5, Ubiquitin, WD repeat-containing protein 5, Set1/Ash2 histone methyltransferase complex subunit ASH2

Experimental Information Details

Resolution: 4.0Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)