EMDB EMD-0649

Single particle reconstruction
3.21Å resolution

Electron cryo-microscopy of the eukaryotic translation initiation factor 2B bound to translation initiation factor 2 from Homo sapiens

Map released:
Overview of EMD-0649
Source organism: Homo sapiens [9606]
Fitted atomic model: 6o81
Related EM entry by publication: EMD-0651, EMD-0664
Primary publication:
eIF2B-catalyzed nucleotide exchange and phosphoregulation by the integrated stress response.
Kenner LR, Anand AA, Nguyen HC, Myasnikov AG, Klose CJ, McGeever LA, Tsai JC, Miller-Vedam LE, Walter P, Frost A
Science 364 491-495 (2019)
PMID: 31048491

Function and Biology Details

Sample name: eIF2B bound to eIF2
Ligand: 2-(4-chloranylphenoxy)-~{N}-[4-[2-(4-chloranylphenoxy)ethanoylamino]cyclohexyl]ethanamide
Proteins: eIF2B bound to eIF2, Translation initiation factor eIF-2B subunit epsilon, Translation initiation factor eIF-2B subunit beta, Translation initiation factor eIF-2B subunit delta, Translation initiation factor eIF-2B subunit alpha, Translation initiation factor eIF-2B subunit gamma, Eukaryotic translation initiation factor 2 subunit 1, Eukaryotic translation initiation factor 2 subunit 3, Translation initiation factor eiF2 beta-subunit

Experimental Information Details

Resolution: 3.21Å
Resolution method: FSC 0.143 CUT-OFF
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)