EMDB EMD-0643

Single particle reconstruction
4.2Å resolution

30S initiation complex

Map released:
Last modified:
Overview of EMD-0643
Source organism: Escherichia coli [562]
Fitted atomic model: 6o7k
Related EM entry by publication: EMD-0661, EMD-0662
Primary publication:
Late steps in bacterial translation initiation visualized using time-resolved cryo-EM.
Kaledhonkar S, Fu Z, Caban K, Li W, Chen B, Sun M, Gonzalez Jr RL, Frank J
Nature 570 400-404 (2019)
PMID: 31108498

Function and Biology Details

Sample name: 70S elongation competent ribosome
Nucleic acids: 16S, tRNA, mRNA
Proteins: 70S elongation competent ribosome, Translation initiation factor IF-1, Translation initiation factor IF-2, 30S ribosomal protein S17, 30S ribosomal protein S10, 30S ribosomal protein S11, 30S ribosomal protein S12, 30S ribosomal protein S13, 30S ribosomal protein S14, 30S ribosomal protein S15, 30S ribosomal protein S16, 30S ribosomal protein S18, 30S ribosomal protein S19, 30S ribosomal protein S2, 30S ribosomal protein S20, 30S ribosomal protein S21, 30S ribosomal protein S3, 30S ribosomal protein S4, 30S ribosomal protein S5, 30S ribosomal protein S6, 30S ribosomal protein S7, 30S ribosomal protein S8, 30S ribosomal protein S9

Experimental Information Details

Resolution: 4.2Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TECNAI F30
Detector: GATAN K2 SUMMIT (4k x 4k)