EMDB EMD-0591

Single particle reconstruction
3.37Å resolution

Architectural principles for Hfq/Crc-mediated regulation of gene expression Hfq-Crc-amiE 2:3:2 complex

Map released:
Overview of EMD-0591
Fitted atomic model: 6o1l
Related EM entry by publication: EMD-0590, EMD-0592
Primary publication:
Architectural principles for Hfq/Crc-mediated regulation of gene expression.
Pei XY, Dendooven T, Sonnleitner E, Chen S, Blasi U, Luisi BF
Elife 8 - (2019)
PMID: 30758287

Function and Biology Details

Sample name: Assembly of Hfq-Crc-amiE with a 2:3:2 stoichiometry
Nucleic acid: RNA (5'-R(*AP*AP*AP*AP*AP*UP*AP*AP*CP*AP*AP*CP*AP*AP*GP*AP*GP*G)-3')
Proteins: Assembly of Hfq-Crc-amiE with a 2:3:2 stoichiometry, Catabolite repression control protein, RNA-binding protein Hfq

Experimental Information Details

Resolution: 3.37Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON III (4k x 4k)