Single particle reconstruction
3.6Å resolution

Cryo-EM Structure of the Lysosomal Folliculin Complex (FLCN-FNIP2-RagA-RagC-Ragulator)

Map released:
Last modified:
Overview of EMD-0554
Source organism: Homo sapiens [9606]
Fitted atomic model: 6nzd
Related EM entry by publication: EMD-0556
Primary publication:
Structural mechanism of a Rag GTPase activation checkpoint by the lysosomal folliculin complex.
Lawrence RE, Fromm SA, Fu Y, Yokom AL, Kim DJ, Thelen AM, Young LN, Lim CY, Samelson AJ, Hurley JH, Zoncu R
Science - (2019)
PMID: 31672913

Function and Biology Details

Sample name: FLCN-FNIP2-RagA-RagC-Ragulator Complex
Ligands: GUANOSINE-5'-DIPHOSPHATE, 9-{5-O-[(S)-hydroxy{[(R)-hydroxy(thiophosphonooxy)phosphoryl]oxy}phosphoryl]-alpha-L-arabinofuranosyl}-3,9-dihydro-1H-purine-2,6-dione
Proteins: FLCN-FNIP2-RagA-RagC-Ragulator Complex, Ragulator complex protein LAMTOR1, Ragulator complex protein LAMTOR2, Ragulator complex protein LAMTOR3, Ragulator complex protein LAMTOR4, Hepatitis B virus x interacting protein, Ras-related GTP-binding protein A, Ras-related GTP-binding protein C, Folliculin, Folliculin-interacting protein 2

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cryoSPARC
Detector: GATAN K2 SUMMIT (4k x 4k)