EMDB EMD-0519

Single particle reconstruction
3.3Å resolution

Small conformation of apo CRISPR_Csm complex

Map released:
Overview of EMD-0519
Source organism: Streptococcus thermophilus [1308]
Fitted atomic model: 6nue
Related EM entries by publication: EMD-0516, EMD-0517, EMD-0518
Primary publication:
Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry.
Guo M, Zhang K, Zhu Y, Pintilie GD, Guan X, Li S, Schmid MF, Ma Z, Chiu W, Huang Z
Cell Res. - (2019)
PMID: 30814678

Function and Biology Details

Sample name: Small conformation of apo CRISPR_Csm complex
Ligand: ADENOSINE-5'-TRIPHOSPHATE
Nucleic acid: crRNA
Proteins: Small conformation of apo CRISPR_Csm complex, CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A), CRISPR system Cms protein Csm2, CRISPR type III-associated RAMP protein Csm3, CRISPR type III-associated RAMP protein Csm4

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)