EMDB EMD-0517

Single particle reconstruction
3.4Å resolution

Big conformation of ssRNA-bound CRISPR_Csm complex

Map released:
Overview of EMD-0517
Source organism: Streptococcus thermophilus [1308]
Related EM entries by publication: EMD-0516, EMD-0518, EMD-0519
Primary publication:
Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry.
Guo M, Zhang K, Zhu Y, Pintilie GD, Guan X, Li S, Schmid MF, Ma Z, Chiu W, Huang Z
Cell Res. - (2019)
PMID: 30814678

Function and Biology Details

Sample name: Big conformation of ssRNA-bound CRISPR_Csm complex
Protein: Big conformation of ssRNA-bound CRISPR_Csm complex

Experimental Information Details

Resolution: 3.4Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: CryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)