EMDB EMD-0516

Single particle reconstruction
3.5Å resolution

Small conformation of ssRNA-bound CRISPR_Csm complex

Map released:
Overview of EMD-0516
Source organism: Streptococcus thermophilus [1308]
Fitted atomic model: 6nud
Related EM entries by publication: EMD-0517, EMD-0518, EMD-0519
Primary publication:
Coupling of ssRNA cleavage with DNase activity in type III-A CRISPR-Csm revealed by cryo-EM and biochemistry.
Guo M, Zhang K, Zhu Y, Pintilie GD, Guan X, Li S, Schmid MF, Ma Z, Chiu W, Huang Z
Cell Res. - (2019)
PMID: 30814678

Function and Biology Details

Sample name: Small conformation of ssRNA-bound CRISPR_Csm complex
Ligand: ADENOSINE-5'-TRIPHOSPHATE
Nucleic acids: crRNA, target ssRNA
Proteins: Small conformation of ssRNA-bound CRISPR_Csm complex, CRISPR system Cms protein Csm2, CRISPR type III-associated RAMP protein Csm4, CRISPR system single-strand-specific deoxyribonuclease Cas10/Csm1 (subtype III-A), CRISPR type III-associated RAMP protein Csm3

Experimental Information Details

Resolution: 3.5Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: cryoSPARC
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)