EMDB EMD-0447

Single particle reconstruction
3.49Å resolution

cryo-EM Structure of the LbCas12a-crRNA-AcrVA1 complex

Map released:
Overview of EMD-0447
Source organisms:
Fitted atomic model: 6nmd
Related EM entries by publication: EMD-0445, EMD-0446, EMD-0449, EMD-20132, EMD-20182, EMD-9398
Primary publication:
Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins.
Zhang H, Li Z, Daczkowski CM, Gabel C, Mesecar AD, Chang L
Cell Host Microbe - (2019)
PMID: 31155345

Function and Biology Details

Sample name: protein complex 2
Ligands: MAGNESIUM ION, water
Nucleic acid: crRNA
Proteins: protein complex 2, Cpf1, AcrVA1

Experimental Information Details

Resolution: 3.49Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: cisTEM
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)