EMDB EMD-0446

Single particle reconstruction
4.24Å resolution

CryoEM structure of the LbCas12a-crRNA-2xAcrVA1 complex

Map released:
Overview of EMD-0446
Source organisms:
Fitted atomic model: 6nmc
Related EM entries by publication: EMD-0445, EMD-0447, EMD-0449, EMD-20132, EMD-20182, EMD-9398
Primary publication:
Structural Basis for the Inhibition of CRISPR-Cas12a by Anti-CRISPR Proteins.
Zhang H, Li Z, Daczkowski CM, Gabel C, Mesecar AD, Chang L
Cell Host Microbe - (2019)
PMID: 31155345

Function and Biology Details

Sample name: protein complex 3
Ligands: MAGNESIUM ION, water
Nucleic acid: RNA
Proteins: protein complex 3, Cpf1, AcrVA1

Experimental Information Details

Resolution: 4.24Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)