EMDB EMD-0370

Single particle reconstruction
3.6Å resolution

Cryo-EM structure of early cytoplasmic-immediate (ECI) pre-60S ribosomal subunit

Map released:
Overview of EMD-0370
Source organism: Baker's yeast [559292]
Fitted atomic model: 6n8k
Related EM entries by publication: EMD-0369, EMD-0371, EMD-0372, EMD-0373, EMD-0374
Primary publication:
Tightly-orchestrated rearrangements govern catalytic center assembly of the ribosome.
Zhou Y, Musalgaonkar S, Johnson AW, Taylor DW
Nat Commun 10 958-958 (2019)
PMID: 30814529

Function and Biology Details

Sample name: Early cytoplasmic-immediate (ECI) pre-60S ribosomal subunit
Ligands: GUANOSINE-5'-TRIPHOSPHATE, MAGNESIUM ION
Nucleic acids: Saccharomyces cerevisiae S288C 35S pre-ribosomal RNA (RDN37-1), miscRNA, 5S rRNA, 5.8S rRNA
Proteins: Early cytoplasmic-immediate (ECI) pre-60S ribosomal subunit, Ribosomal Protein uL1, 60S ribosomal export protein NMD3, 60S ribosomal protein L2-A, 60S ribosomal protein L28, 60S ribosomal protein L3, Nucleolar GTP-binding protein 1, 60S ribosomal protein L4-A, 60S ribosomal protein L30, 60S ribosomal protein L31-A, 60S ribosomal protein L6-A, 60S ribosomal protein L32, 60S ribosomal protein L7-A, 60S ribosomal protein L33-A, 60S ribosomal protein L8-A, 60S ribosomal protein L34-A, Bud site selection protein 20, 60S ribosomal protein L9-A, 60S ribosomal protein L35-A, 60S ribosomal protein L36-A, 60S ribosomal protein L37-A, 60S ribosomal protein L38, 60S ribosomal protein L13-A, 60S ribosomal protein L39, 60S ribosomal protein L14-A, 60S ribosomal protein L43-A, 60S ribosomal protein L15-A, Ribosome biogenesis protein RLP24, 60S ribosomal protein L16-A, 60S ribosomal protein L17-A, 60S ribosomal protein L18-A, 60S ribosomal protein L19-A, 60S ribosomal protein L20-A, 60S ribosomal protein L21-A, 60S ribosomal protein L22-A, 60S ribosomal protein L23-A, Ribosome assembly factor MRT4, 60S ribosomal protein L25, 60S ribosomal protein L26-A, Eukaryotic translation initiation factor 6, 60S ribosomal protein L27-A, UPF0642 protein YBL028C, 60S ribosomal protein L5, 60S ribosomal protein L11-A, 60S ribosomal protein L42-A, 60S ribosomal protein L29

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)