EMDB EMD-0360

Single particle reconstruction
3.6Å resolution

S. cerevisiae spliceosomal E complex (UBC4)

Map released:
Last modified:
Overview of EMD-0360
Source organisms:
Fitted atomic model: 6n7p
Related EM entry by publication: EMD-0361
Primary publication:
A unified mechanism for intron and exon definition and back-splicing.
Li X, Liu S, Zhang L, Issaian A, Hill RC, Espinosa S, Shi S, Cui Y, Kappel K, Das R, Hansen KC, Zhou ZH, Zhao R
Nature 573 375-380 (2019)
PMID: 31485080

Function and Biology Details

Sample name: Saccharomyces cerevisiae spliceosomal E complex (EBC4)
Ligand: ZINC ION
Nucleic acids: U1 snRNA, UBC4 pre-mRNA
Proteins: Saccharomyces cerevisiae spliceosomal E complex (EBC4), U1 small nuclear ribonucleoprotein 70 kDa homolog, U1 small nuclear ribonucleoprotein C, U1 small nuclear ribonucleoprotein A, U1 small nuclear ribonucleoprotein component PRP42, Pre-mRNA-processing factor 39, Protein NAM8, 56 kDa U1 small nuclear ribonucleoprotein component, U1 small nuclear ribonucleoprotein component SNU71,U1 small nuclear ribonucleoprotein component SNU71,Snu71, Protein LUC7, Pre-mRNA-processing protein PRP40, Small nuclear ribonucleoprotein-associated protein B, Small nuclear ribonucleoprotein Sm D1, Small nuclear ribonucleoprotein Sm D2, Small nuclear ribonucleoprotein Sm D3, Small nuclear ribonucleoprotein E, Small nuclear ribonucleoprotein F, Small nuclear ribonucleoprotein G, Nuclear cap-binding protein complex subunit 1, Nuclear cap-binding protein subunit 2

Experimental Information Details

Resolution: 3.6Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)