EMDB EMD-0322

Single particle reconstruction
3.2Å resolution

TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel

Map released:
Overview of EMD-0322
Source organisms:
Fitted atomic model: 6i0y
Primary publication:
Folding pathway of an Ig domain is conserved on and off the ribosome.
Tian P, Steward A, Kudva R, Su T, Shilling PJ, Nickson AA, Hollins JJ, Beckmann R, von Heijne G, Clarke J, Best RB
Proc. Natl. Acad. Sci. U.S.A. 115 E11284-E11293 (2018)
PMID: 30413621

Function and Biology Details

Sample name: TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel
Ligands: MAGNESIUM ION, ZINC ION, TRYPTOPHAN, water
Nucleic acids: 23S ribosomal RNA, 5S ribosomal RNA, Proline tRNA
Proteins: TnaC-stalled ribosome complex with the titin I27 domain folding close to the ribosomal exit tunnel, ribosome, Tryptophanase operon leader peptide, Titin nascent chain, 50S ribosomal protein L24, 50S ribosomal protein L32, 50S ribosomal protein L33, 50S ribosomal protein L34, 50S ribosomal protein L35, 50S ribosomal protein L36, 50S ribosomal protein L10, 50S ribosomal protein L7/L12, Tryptophanase operon leader peptide, 50S ribosomal protein L25, 50S ribosomal protein L2, 50S ribosomal protein L3, 50S ribosomal protein L4, 50S ribosomal protein L5, 50S ribosomal protein L6, 50S ribosomal protein L9, 50S ribosomal protein L11, 50S ribosomal protein L13, 50S ribosomal protein L14, 50S ribosomal protein L15, 50S ribosomal protein L16, 50S ribosomal protein L17, 50S ribosomal protein L18, 50S ribosomal protein L19, 50S ribosomal protein L20, 50S ribosomal protein L21, 50S ribosomal protein L22, 50S ribosomal protein L23, 50S ribosomal protein L27, 50S ribosomal protein L28, 50S ribosomal protein L29, 50S ribosomal protein L30, Titin

Experimental Information Details

Resolution: 3.2Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 QUANTUM (4k x 4k)