EMDB EMD-0141

Helical reconstruction
3.3Å resolution

Cryo-EM structure of in vitro assembled Measles virus N into nucleocapsid-like particles (NCLPs) bound to polyA RNA hexamers.

Map released:
Overview of EMD-0141
Source organisms:
Fitted atomic model: 6h5q
Primary publication:
Assembly and cryo-EM structures of RNA-specific measles virus nucleocapsids provide mechanistic insight into paramyxoviral replication.
Desfosses A, Milles S, Jensen MR, Guseva S, Colletier JP, Maurin D, Schoehn G, Gutsche I, Ruigrok RWH, Blackledge M
Proc. Natl. Acad. Sci. U.S.A. - (2019)
PMID: 30787192

Function and Biology Details

Sample name: Measles virus Nucleoprotein assembled into nucleocapsid-like particles (NCLPs) bound to polyA RNA hexamers.
Nucleic acid: RNA (5'-R(*AP*AP*AP*AP*AP*A)-3')
Proteins: Measles virus Nucleoprotein assembled into nucleocapsid-like particles (NCLPs) bound to polyA RNA hexamers., Measles virus Nucleoprotein, polyA RNA, Nucleocapsid

Experimental Information Details

Resolution: 3.3Å
Resolution method: FSC 0.143 CUT-OFF
Reconstruction software: RELION
Microscope: FEI POLARA 300
Detector: GATAN K2 SUMMIT (4k x 4k)