EMDB EMD-0136

Single particle reconstruction
3.25Å resolution

Electron cryo-microscopy of VgrG1 in the Type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lipid nanodiscs

Map released:
Last modified:
Overview of EMD-0136
Source organism: Pseudomonas aeruginosa PAO1 [208964]
Fitted atomic model: 6h3n
Related EM entry by publication: EMD-0135
Primary publication:
Mechanism of loading and translocation of type VI secretion system effector Tse6.
Quentin D, Ahmad S, Shanthamoorthy P, Mougous JD, Whitney JC, Raunser S
Nat Microbiol 3 1142-1152 (2018)
PMID: 30177742

Function and Biology Details

Sample name: The type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lipid nanodiscs
Proteins: The type VI secretion VgrG1-Tse6-EF-Tu complex embedded in lipid nanodiscs, VgrG1

Experimental Information Details

Resolution: 3.25Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C3
Reconstruction software: SPHIRE
Microscope: FEI TITAN KRIOS
Detector: GATAN K2 SUMMIT (4k x 4k)