EMDB EMD-0055

Single particle reconstruction
4.3Å resolution

Cryo-EM reconstruction of yeast 80S ribosome in complex with mRNA, tRNA and eEF2 (GDP+AlF4/sordarin): SSU-focused map

Map released:
Last modified:
Overview of EMD-0055
Source organism: Saccharomyces cerevisiae [4932]
Related EM entries by publication: EMD-0047, EMD-0048, EMD-0049
Primary publication:
Structural Insights into the Role of Diphthamide on Elongation Factor 2 in mRNA Reading-Frame Maintenance.
Pellegrino S, Demeshkina N, Mancera-Martinez E, Melnikov S, Simonetti A, Myasnikov A, Yusupov M, Yusupova G, Hashem Y
J. Mol. Biol. 430 2677-2687 (2018)
PMID: 29886014

Function and Biology Details

Sample name: 80S ribosome in complex with mRNA, tRNA and eEF2 in presence of GDP, AlF4 and anti-fungal drug (sordarin)
Proteins: 80S ribosome in complex with mRNA, tRNA and eEF2 in presence of GDP, AlF4 and anti-fungal drug (sordarin), 80S ribosome, mRNA

Experimental Information Details

Resolution: 4.3Å
Resolution method: FSC 0.143 CUT-OFF
Applied symmetry: C1
Microscope: FEI TITAN KRIOS
Detector: FEI FALCON II (4k x 4k)