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            "id": "SRS372045",
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                "latitude": null,
                "biosample": "SAMN01779589",
                "longitude": null,
                "sample-metadata": [
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                        "key": "NCBI sample classification",
                        "value": "410658",
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                "accession": "SRS372045",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Sampling in \"Criadero Klein\" farm located in Rolling Pampas, Argentina (-35* 7*17,4 \", -60* 15* 42,6\") was performed in June 2010. Rolling Pampas Soils are classified as Typical Argiudolls and mean annual precipitation and temperature are 1002 mm and 16.8 *C. Sampling was performed on bulk soils from non-agricultural plots.  Non-agricultural samples were obtained from grassland areas covered with herbaceous (non-woody) plants common in the region such as Cirsium sp, Trifolium sp, Micropsis sp, Festuca sp, Dichondra sp, Cyperus sp and Taraxacum officinale among others grasses, nearby the farmhouses. Soil cores were taken with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 in each plot. Cores were then pooled together. Soil samples were stored at -80*C until processing. Total DNA was obtained using PowerSoil DNA isolation kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Rolling Pampas Soil: Criadero Klein Non Agriculture",
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                "species": null,
                "last-update": "2016-04-14T10:00:10"
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            "type": "samples",
            "id": "SRS372386",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779590",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
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                        "key": "instrument model",
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                "accession": "SRS372386",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Balcarce sampling location was a 34-year crop rotation experiment located in the Southern Pampas (-37º45´09´´, -58º17´47´´). Soils in Balcarce are classified as a complex of TypicalArgiudolls and Petrocalcic/Paleudols and mean annual precipitation and temperatures are 875 mm and 13.8 ºC. The experiment was carried out as a randomized complete block design with a split-plot treatment arrangement, three replicates, and 10 by 35 m experimental units. Treatments assigned to main plots were referred to as no-till or conventional tillage (undermould board plough). This SRA sample corresponds to No Till samples only. Rotations have included pastures and grain crops during the last 16 years. Last rotation before sampling was corn-soybean. Samples were collected on August 2010 from all treatments and replicates, a month after soybean harvest. Soil cores were taken with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 in each plot. Cores were then pooled together.Composite samples were immediately sent to the lab where they were air dried and sieved through 1 mm mesh to thoroughly homogenize, break aggregates and remove roots and plant detritus. Total DNA was extracted using PowerSoil kit from MoBio.",
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                "sample-name": "Argentina Balcarce Soil: Balcarce No Till Farming",
                "sample-alias": "Balcarce Agriculture (No Till)",
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                "species": null,
                "last-update": "2016-04-14T10:00:10"
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            "type": "samples",
            "id": "SRS372411",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779633",
                "longitude": null,
                "sample-metadata": [
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                        "key": "NCBI sample classification",
                        "value": "410658",
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                        "value": "454 GS FLX Titanium",
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                "accession": "SRS372411",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Balcarce sampling location was a 34-year crop rotation experiment located in the Southern Pampas (-37º45´09´´, -58º17´47´´). Soils in Balcarce are classified as a complex of TypicalArgiudolls and Petrocalcic/Paleudols and mean annual precipitation and temperatures are 875 mm and 13.8 ºC. The experiment was carried out as a randomized complete block design with a split-plot treatment arrangement, three replicates, and 10 by 35 m experimental units. Treatments assigned to main plots were referred to as no-till or conventional tillage (undermould board plough). This SRA sample corresponds to Conventional Tillage samples only. Rotations have included pastures and grain crops during the last 16 years. Last rotation before sampling was corn-soybean. Samples were collected on August 2010 from all treatments and replicates, a month after soybean harvest. Soil cores were taken with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 in each plot. Cores were then pooled together.Composite samples were immediately sent to the lab where they were air dried and sieved through 1 mm mesh to thoroughly homogenize, break aggregates and remove roots and plant detritus. Total DNA was extracted using PowerSoil kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Balcarce Soil: Balcarce Conventional Tillage",
                "sample-alias": "Balcarce Agriculture (Conventional Tillage)",
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                "species": null,
                "last-update": "2016-04-14T10:00:10"
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        {
            "type": "samples",
            "id": "SRS372412",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779634",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
                        "unit": null
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                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
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                ],
                "accession": "SRS372412",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Balcarce sampling location was a 34-year crop rotation experiment located in the Southern Pampas (-37*45*09**, -58*17*47**). Soils in Balcarce are classified as a complex of TypicalArgiudolls and Petrocalcic/Paleudols and mean annual precipitation and temperatures are 875 mm and 13.8 *C. Soil samples were taken in triplicate nearby the farmhouses where no land use has been recorded in the last 30 years except from grass mowing. This area was covered with grass and other herbaceous (non-woody) plants common in the region. Samples were collected on August 2010. Soil cores were taken with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 in each plot. Cores were then pooled together.Composite samples were immediately sent to the lab where they were air dried and sieved through 1 mm mesh to thoroughly homogenize, break aggregates and remove roots and plant detritus. Total DNA was extracted using PowerSoil kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
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                "sample-name": "Argentina Balcarce Soil: Balcarce Non Agriculture",
                "sample-alias": "Balcarce Non Agriculture",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:10"
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        },
        {
            "type": "samples",
            "id": "SRS372415",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779637",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
                        "unit": null
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                    {
                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
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                ],
                "accession": "SRS372415",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Anguil sampling site (-36* 35' 31.56\", -63* 57' 31.05\") is located in the Middle-West Argentinean Pampas, an area with entic haplustolls soils. Mean annual precipitation and temperature are 760 mm and 15.3 *C. Sampling was performed on September 2011 and wheat was recently planted in all plots under agricultural management. Three different distant areas within one 12 ha plot under no-till farming (NT) during 4 years and more that 15 years under agricultural treatment were sampled. Bulk soils were obtained from NT sampling sites with an auger from the upper 20 cm soil layer, from 20 randomly selected spots on each of the three selected areas considered as independent samples. Replicates for each plot were then pooled together and sent to the lab for further processing. Soil samples were stored at -80*C until processing. Total DNA was obtained from all bulk using PowerSoil DNA isolation kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Anguil Bulk Soil: Anguil Bulk No Till Farming",
                "sample-alias": "Anguil Bulk Agriculture (No Till)",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:10"
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            "id": "SRS372416",
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                "latitude": null,
                "biosample": "SAMN01779638",
                "longitude": null,
                "sample-metadata": [
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                        "key": "NCBI sample classification",
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                "accession": "SRS372416",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Anguil sampling site (-36* 35' 31.56\", -63* 57' 31.05\") is located in an area with entic haplustolls soils. Mean annual precipitation and temperature are 760 mm and 15.3 *C. Sampling was performed on September 2011 and wheat was recently planted in all plots under agricultural management. Three different plots under conventional tillage (CT) for the last 60 years were sampled. Bulk soils were obtained from CT sampling sites with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 for each plot. Replicates for each plot were then pooled together and sent to the lab for further processing. Total DNA was obtained from all bulk using PowerSoil DNA isolation kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
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                "sample-name": "Argentina Anguil Bulk Soil: Anguil Bulk Conventional Tillage",
                "sample-alias": "Anguil Bulk Agriculture (Conventional Tillage)",
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                "species": null,
                "last-update": "2016-04-14T10:00:10"
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            "type": "samples",
            "id": "SRS372421",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779643",
                "longitude": null,
                "sample-metadata": [
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                        "key": "NCBI sample classification",
                        "value": "410658",
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                "accession": "SRS372421",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Anguil sampling site (-36* 35' 31.56\", -63* 57' 31.05\") is located in an area with entic haplustolls soils. Mean annual precipitation and temperature are 760 mm and 15.3 *C. Sampling was performed on September 2011. Two plots covered by weeping lovegrass (Eragrostis curvula) for the last 60 years and one other plot covered by herbaceous non-woody plants typical from the area were sampled as No Agriculture (NA) control. Bulk soils were obtained with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 for each plot. Replicates for each plot were then pooled together and sent to the lab for further processing. Soil samples were stored at -80*C until processing. Total DNA was obtained from all bulk samples using PowerSoil DNA isolation kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Anguil Bulk Soil: Anguil Bulk Non Agriculture",
                "sample-alias": "Anguil Bulk Non Agriculture",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:10"
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        },
        {
            "type": "samples",
            "id": "SRS372468",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779690",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
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                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
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                "accession": "SRS372468",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Anguil sampling site (-36* 35' 31.56\", -63* 57' 31.05\") is located in an area with entic haplustolls soils. Mean annual precipitation and temperature are 760 mm and 15.3 *C. Sampling was performed on September 2011 and wheat was recently planted in all plots under agricultural management. Rhizospheric soil from wheat plants from three different plots under conventional tillage (CT) for the last 60 years were sampled. Plants and surrounding soil were taken with a shovel and sent to the lab where soil loosely attached to roots was disaggregated with gloved hands and finally soils attached to roots were obtained by brush scrapping. Rhizospheric soil from wheat plants on each CT sampled spot were prepared separately. Soil samples were stored at -80*C until processing. Total DNA was obtained from rhizospheric samples using PowerSoil DNA isolation kit from MoBio",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Anguil Rhizospheric Soil: Anguil Rhizosphere Conventional Tillage",
                "sample-alias": "Anguil Rhizospheric Soil Agriculture (Conventional Tillage)",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:10"
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        {
            "type": "samples",
            "id": "SRS372472",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779694",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
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                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
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                "accession": "SRS372472",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Anguil sampling site (-36* 35' 31.56\", -63* 57' 31.05\") is located in an area with entic haplustolls soils. Mean annual precipitation and temperature are 760 mm and 15.3 *C. Sampling was performed on September 2011 and wheat was recently planted in all plots under agricultural management. Rhizospheric soil from wheat plants in one plot under no-till farming (NT) during 4 years and more that 15 years under agricultural treatment were sampled. Sampling was performed on three different distant areas within the only plot available for this treatment (12 ha) and were considered as independent plots. Plants and surrounding soil were taken with a shovel and sent to the lab where soil loosely attached to roots was disaggregated with gloved hands and finally soils attached to roots were obtained by brush scrapping. Soil samples were stored at -80*C until processing. Total DNA was obtained from rhizospheric samples using PowerSoil DNA isolation kit from MoBio",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Anguil Rhizospheric Soil: Anguil Rhizosphere No Till",
                "sample-alias": "Anguil Rhizospheric Soil Agriculture (No Till)",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:10"
            },
            "relationships": {
                "runs": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372472/runs?format=api"
                    }
                },
                "biome": {
                    "data": {
                        "type": "biomes",
                        "id": "root:Environmental:Terrestrial:Soil"
                    },
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
                    }
                },
                "studies": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372472/studies?format=api"
                    },
                    "data": [
                        {
                            "type": "studies",
                            "id": "MGYS00000666",
                            "links": {
                                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000666?format=api"
                            }
                        }
                    ]
                }
            },
            "links": {
                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372472?format=api"
            }
        },
        {
            "type": "samples",
            "id": "SRS372823",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01780144",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
                        "unit": null
                    },
                    {
                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
                    }
                ],
                "accession": "SRS372823",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Anguil sampling site (-36° 35' 31.56\", -63° 57' 31.05\") is located in an area with entic haplustolls soils. Mean annual precipitation and temperature are 760 mm and 15.3 ºC. Sampling was performed on September 2011 and wheat was recently planted in all plots under agricultural management. Three different plots under conventional tillage (CT) for the last 60 years and three different areas within one 12 ha plot under no-till farming (NT) during 4 years and more that 15 years under agricultural treatment were sampled. Rhizospheric soil from wheat plants on each NT and CT sampled spot was prepared. Plants and surrounding soil were taken with a shovel and sent to the lab where soil loosely attached to roots was disaggregated with gloved hands and finally soils attached to roots were obtained by brush scrapping. Soil samples were stored at -80°C until processing. Total DNA was obtained from all rhizospheric samples using PowerSoil DNA isolation kit from MoBio. Total RNA was obtained from NT and CT rhizospheric samples using PowerSoil Total RNA purification kit from MoBio. Single strand cDNA (cDNAss) was prepared from total RNA for further PCR amplification of pyrotags using first-strand cDNA synthesis system kit from Roche. Purification of prokaryotic mRNA was performed using MicrobeExpress kit from Ambion and double-stranded cDNA was prepared from mRNA using cDNA synthesis system kit from Roche.   Pyrotags from gDNA and cDNA were generated by 16S rDNA V4 region amplification using F563-R802 primers developed by Ribosomal Database Project.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Anguil Rhizospheric Soil: All sample amplicons from cDNA and gDNA",
                "sample-alias": "Anguil Rhizosphere 16S Amplicons",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:10"
            },
            "relationships": {
                "runs": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372823/runs?format=api"
                    }
                },
                "biome": {
                    "data": {
                        "type": "biomes",
                        "id": "root:Environmental:Terrestrial:Soil"
                    },
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
                    }
                },
                "studies": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372823/studies?format=api"
                    },
                    "data": [
                        {
                            "type": "studies",
                            "id": "MGYS00000666",
                            "links": {
                                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000666?format=api"
                            }
                        }
                    ]
                }
            },
            "links": {
                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372823?format=api"
            }
        },
        {
            "type": "samples",
            "id": "SRS386929",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01893943",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
                        "unit": null
                    },
                    {
                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
                    }
                ],
                "accession": "SRS386929",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Anguil sampling site (-36° 35' 31.56\", -63° 57' 31.05\") is located in an area with entic haplustolls soils. Mean annual precipitation and temperature are 760 mm and 15.3 ºC. Sampling was performed on September 2011 and wheat was recently planted in all plots under agricultural management. Three different plots under conventional tillage (CT) for the last 60 years and three different areas within one 12 ha plot under no-till farming (NT) during 4 years and more that 15 years under agricultural treatment were sampled. In addition, two plots covered by weeping lovegrass (Eragrostis curvula) for the last 60 years and one other plot covered by herbaceous non-woody plants typical from the area were sampled as No Agriculture (NA) control. Bulk soils were obtained with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 for each plot. Replicates for each plot were then pooled together and sent to the lab for further processing. Soil samples were stored at -80*C until processing. Total DNA was obtained from all bulk samples using PowerSoil DNA isolation kit from MoBio. Pyrotags were generated by 16S rDNA V4 region amplification using F563-R802 primers developed by Ribosomal Database Project.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Anguil Bulk Soil: All amplicon samples from 16S rDNA",
                "sample-alias": "Anguil Bulk 16S Amplicons",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:10"
            },
            "relationships": {
                "runs": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS386929/runs?format=api"
                    }
                },
                "biome": {
                    "data": {
                        "type": "biomes",
                        "id": "root:Environmental:Terrestrial:Soil"
                    },
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
                    }
                },
                "studies": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS386929/studies?format=api"
                    },
                    "data": [
                        {
                            "type": "studies",
                            "id": "MGYS00000666",
                            "links": {
                                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000666?format=api"
                            }
                        }
                    ]
                }
            },
            "links": {
                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS386929?format=api"
            }
        },
        {
            "type": "samples",
            "id": "SRS650217",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN02898036",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "geographic location (longitude)",
                        "value": "- 32.94105, -61.844164",
                        "unit": null
                    },
                    {
                        "key": "geographic location (depth)",
                        "value": "0-20cm",
                        "unit": null
                    },
                    {
                        "key": "geographic location (country and/or sea,region)",
                        "value": "Cruz Alta",
                        "unit": null
                    },
                    {
                        "key": "collection date",
                        "value": "may-12",
                        "unit": null
                    },
                    {
                        "key": "environment (biome)",
                        "value": "soil",
                        "unit": null
                    },
                    {
                        "key": "environment (feature)",
                        "value": "soil",
                        "unit": null
                    },
                    {
                        "key": "elevation",
                        "value": "0",
                        "unit": null
                    },
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
                        "unit": null
                    },
                    {
                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
                    }
                ],
                "accession": "SRS650217",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "MIMS Environmental/Metagenome sample from soil metagenome",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "MIMS Environmental/Metagenome sample from soil metagenome",
                "sample-alias": "CruzAlta",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:10"
            },
            "relationships": {
                "runs": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS650217/runs?format=api"
                    }
                },
                "biome": {
                    "data": {
                        "type": "biomes",
                        "id": "root:Environmental:Terrestrial:Soil"
                    },
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
                    }
                },
                "studies": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS650217/studies?format=api"
                    },
                    "data": [
                        {
                            "type": "studies",
                            "id": "MGYS00000666",
                            "links": {
                                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000666?format=api"
                            }
                        }
                    ]
                }
            },
            "links": {
                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS650217?format=api"
            }
        },
        {
            "type": "samples",
            "id": "SRS353016",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01096866",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "2",
                        "unit": null
                    },
                    {
                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
                    }
                ],
                "accession": "SRS353016",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Soil samples were collected between June and August 2010 at two different locations in Argentine Pampas. Sampling was done in production fields and in experimental plots. Production plots were sampled in three different farms located in the Rolling Pampas: La Estrella, La Negrita and Criadero Klein.   Rolling Pampas Soils are classified as Typic Argiudolls (USDA 1975) and mean annual precipitation and temperature are 1002 mm and 16.8 ºC. Production soils were under agricultural management for at least one century, with a mixed rotation of pastures and annual grain crops during the first decades and only grain crops for the last 30 years. Soils were managed under no-till for the last 10-15 years. The last crop rotation was wheat-soybean. Nitrogen fertilization was performed. Samples were collected a month after soybean harvest on June 2010. Soil samples were also collected nearby the farmers’ houses where no land use has been recorded in the last 30 years except from grass mowing. Parks around farmers' houses are usually considered as undisturbed (i.e. no land use) environments in Argentine Pampas (Vazquez et al. 1990). Soils under no land use were covered with grass and other herbaceous (non-woody) plants common in the region such as Cirsium sp, Trifolium sp, Micropsis sp, Festuca sp, Dichondra sp, Cyperus sp and Taraxacum officinale. Soil cores were taken with an auger from the upper 20 cm soil layer, from 20 randomly selected locations within an area of ~500 m2 in each plot. Cores were then pooled together.   The second sampling location was a 34 year- crop rotation experiment located in Balcarce in the Southern Pampas. Soils in Balcarce are classified as a complex of Typic Argiudolls and Petrocalcic Paleudols and mean annual precipitation and temperatures are 875 mm and 13.8 ºC. The experiment was carried out as a randomized complete block design with a split-plot treatment arrangement, three replicates, and 10 by 35 m experimental units. Treatments assigned to main plots were referred to as no-till or conventional tillage (under mouldboard plough). Rotations have included pastures and grain crops during the last 16 years. Nitrogen fertilization was performed (90 kg N ha-1). Last rotation before sampling was corn-soybean. Samples were collected from all treatments and replicates on August 2010, a month after soybean harvest. Soil samples were also taken in triplicate nearby the farm houses where no land use has been recorded in the last 30 years except from grass mowing. This area was covered with grass and other herbaceous (non-woody) plants common in the region. Samples were collected as in Rolling Pampa. Composite samples were immediately sent to the lab where they were air dried for 2/3 days and sieved through 1 mm mesh to thoroughly homogenize, break aggregates and remove roots and plant detritus. Samples were then stored at -80 °C.  DNA was isolated from 10 g of soil using the PowerMaxSoil DNA Isolation Kit following the manufacturer’s instructions (MO BIO Laboratories, Inc, CA, USA). Technical replicates were defined by performing up to 4 independent extractions per sample. A total of 42 DNA samples were used. The V4 hyper variable region of the 16s rRNA gene was amplified in duplicate PCR reactions using 520F and 802R bacterial universal primers. Amplicons were pooled and equimolar concentrations pyrosequenced at INDEAR (Argentina). on a Roche FLX 454 pyrosequencer running the Titanium chemistry",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentinean Pampa soil 16S pyrotags",
                "sample-alias": "All 68 Samples together",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:09"
            },
            "relationships": {
                "runs": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS353016/runs?format=api"
                    }
                },
                "biome": {
                    "data": {
                        "type": "biomes",
                        "id": "root:Environmental:Terrestrial:Soil"
                    },
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
                    }
                },
                "studies": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS353016/studies?format=api"
                    },
                    "data": [
                        {
                            "type": "studies",
                            "id": "MGYS00000666",
                            "links": {
                                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000666?format=api"
                            }
                        }
                    ]
                }
            },
            "links": {
                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS353016?format=api"
            }
        },
        {
            "type": "samples",
            "id": "SRS371983",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779526",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
                        "unit": null
                    },
                    {
                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
                    }
                ],
                "accession": "SRS371983",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Sampling in \"La Estrella\" farm located in Rolling Pampas, Argentina (-34* 43', -60* 4* 45\") was performed in June 2010. Rolling Pampas Soils are classified as Typical Argiudolls and mean annual precipitation and temperature are 1002 mm and 16.8 *C. Sampling was performed on bulk soils from agricultural plots.  Soils were under agricultural management for the last 30 years and managed by No Till farming the last 16 years using a Wheat-Soybean/Corn rotation. The last crop rotation before sampling was wheat-soybean and samples were taken a month after soybean harvest. Soil cores were taken with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 in each plot. Cores were then pooled together. Soil samples were stored at -80*C until processing. Total DNA was obtained using PowerSoil DNA isolation kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Rolling Pampas Soil: La Estrella Agriculture (No Till)",
                "sample-alias": "La Estrella Agriculture",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:09"
            },
            "relationships": {
                "runs": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS371983/runs?format=api"
                    }
                },
                "biome": {
                    "data": {
                        "type": "biomes",
                        "id": "root:Environmental:Terrestrial:Soil"
                    },
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
                    }
                },
                "studies": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS371983/studies?format=api"
                    },
                    "data": [
                        {
                            "type": "studies",
                            "id": "MGYS00000666",
                            "links": {
                                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000666?format=api"
                            }
                        }
                    ]
                }
            },
            "links": {
                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS371983?format=api"
            }
        },
        {
            "type": "samples",
            "id": "SRS371984",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779527",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
                        "unit": null
                    },
                    {
                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
                    }
                ],
                "accession": "SRS371984",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Sampling in \"La Estrella\" farm located in Rolling Pampas, Argentina (-34* 43', -60* 4* 45\") was performed in June 2010. Rolling Pampas Soils are classified as Typical Argiudolls and mean annual precipitation and temperature are 1002 mm and 16.8 *C. Sampling was performed on bulk soils from non-agricultural plots.  Non-agricultural samples were obtained from grassland areas covered with herbaceous (non-woody) plants common in the region such as Cirsium sp, Trifolium sp, Micropsis sp, Festuca sp, Dichondra sp, Cyperus sp and Taraxacum officinale among others grasses, nearby the farmhouses. Soil cores were taken with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 in each plot. Cores were then pooled together. Soil samples were stored at -80*C until processing. Total DNA was obtained using PowerSoil DNA isolation kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Rolling Pampas Soil: La Estrella Non Agriculture",
                "sample-alias": "La Estrella Non Agriculture",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:09"
            },
            "relationships": {
                "runs": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS371984/runs?format=api"
                    }
                },
                "biome": {
                    "data": {
                        "type": "biomes",
                        "id": "root:Environmental:Terrestrial:Soil"
                    },
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
                    }
                },
                "studies": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS371984/studies?format=api"
                    },
                    "data": [
                        {
                            "type": "studies",
                            "id": "MGYS00000666",
                            "links": {
                                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000666?format=api"
                            }
                        }
                    ]
                }
            },
            "links": {
                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS371984?format=api"
            }
        },
        {
            "type": "samples",
            "id": "SRS372042",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779586",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
                        "unit": null
                    },
                    {
                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
                    }
                ],
                "accession": "SRS372042",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Sampling in \"La Negrita\" farm located in Rolling Pampas, Argentina (-34* 34* 46,2\", -59* 55* 8,4\") was performed in June 2010. Rolling Pampas Soils are classified as Typical Argiudolls and mean annual precipitation and temperature are 1002 mm and 16.8 *C. Sampling was performed on bulk soils from agricultural plots.  Soils were under agricultural management for the last 30 years and managed by No Till farming the last 16 years using a Wheat-Soybean/Corn rotation. The last crop rotation before sampling was wheat-soybean and samples were taken a month after soybean harvest. Soil cores were taken with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 in each plot. Cores were then pooled together. Soil samples were stored at -80*C until processing. Total DNA was obtained using PowerSoil DNA isolation kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Rolling Pampas Soil: La Negrita Agriculture (No Till)",
                "sample-alias": "La Negrita Agriculture",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:09"
            },
            "relationships": {
                "runs": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372042/runs?format=api"
                    }
                },
                "biome": {
                    "data": {
                        "type": "biomes",
                        "id": "root:Environmental:Terrestrial:Soil"
                    },
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
                    }
                },
                "studies": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372042/studies?format=api"
                    },
                    "data": [
                        {
                            "type": "studies",
                            "id": "MGYS00000666",
                            "links": {
                                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000666?format=api"
                            }
                        }
                    ]
                }
            },
            "links": {
                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372042?format=api"
            }
        },
        {
            "type": "samples",
            "id": "SRS372043",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779587",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
                        "unit": null
                    },
                    {
                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
                    }
                ],
                "accession": "SRS372043",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Sampling in \"La Negrita\" farm located in Rolling Pampas, Argentina (-34* 34* 46,2\", -59* 55* 8,4\") was performed in June 2010. Rolling Pampas Soils are classified as Typical Argiudolls and mean annual precipitation and temperature are 1002 mm and 16.8 *C. Sampling was performed on bulk soils from non-agricultural plots.  Non-agricultural samples were obtained from grassland areas covered with herbaceous (non-woody) plants common in the region such as Cirsium sp, Trifolium sp, Micropsis sp, Festuca sp, Dichondra sp, Cyperus sp and Taraxacum officinale among others grasses, nearby the farmhouses. Soil cores were taken with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 in each plot. Cores were then pooled together. Soil samples were stored at -80*C until processing. Total DNA was obtained using PowerSoil DNA isolation kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Rolling Pampas Soil: La Negrita Non Agriculture",
                "sample-alias": "La Negrita Non Agriculture",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:09"
            },
            "relationships": {
                "runs": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372043/runs?format=api"
                    }
                },
                "biome": {
                    "data": {
                        "type": "biomes",
                        "id": "root:Environmental:Terrestrial:Soil"
                    },
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
                    }
                },
                "studies": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372043/studies?format=api"
                    },
                    "data": [
                        {
                            "type": "studies",
                            "id": "MGYS00000666",
                            "links": {
                                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000666?format=api"
                            }
                        }
                    ]
                }
            },
            "links": {
                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372043?format=api"
            }
        },
        {
            "type": "samples",
            "id": "SRS372044",
            "attributes": {
                "latitude": null,
                "biosample": "SAMN01779588",
                "longitude": null,
                "sample-metadata": [
                    {
                        "key": "NCBI sample classification",
                        "value": "410658",
                        "unit": null
                    },
                    {
                        "key": "instrument model",
                        "value": "454 GS FLX Titanium",
                        "unit": null
                    }
                ],
                "accession": "SRS372044",
                "analysis-completed": "2016-04-14",
                "collection-date": null,
                "geo-loc-name": null,
                "sample-desc": "Sampling in \"Criadero Klein\" farm located in Rolling Pampas, Argentina (-35* 7*17,4 \", -60* 15* 42,6\") was performed in June 2010. Rolling Pampas Soils are classified as Typical Argiudolls and mean annual precipitation and temperature are 1002 mm and 16.8 *C. Sampling was performed on bulk soils from agricultural plots.  Soils were under agricultural management for the last 30 years and managed by No Till farming the last 16 years using a Wheat-Soybean/Corn rotation. The last crop rotation before sampling was wheat-soybean and samples were taken a month after soybean harvest. Soil cores were taken with an auger from the upper 20 cm soil layer, from 20 randomly selected spots within an area of ~500 m2 in each plot. Cores were then pooled together. Soil samples were stored at -80*C until processing. Total DNA was obtained using PowerSoil DNA isolation kit from MoBio.",
                "environment-biome": null,
                "environment-feature": null,
                "environment-material": null,
                "sample-name": "Argentina Rolling Pampas Soil: Criadero Klein Agriculture (No Till)",
                "sample-alias": "Criadero Klein Agriculture",
                "host-tax-id": null,
                "species": null,
                "last-update": "2016-04-14T10:00:09"
            },
            "relationships": {
                "runs": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372044/runs?format=api"
                    }
                },
                "biome": {
                    "data": {
                        "type": "biomes",
                        "id": "root:Environmental:Terrestrial:Soil"
                    },
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
                    }
                },
                "studies": {
                    "links": {
                        "related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372044/studies?format=api"
                    },
                    "data": [
                        {
                            "type": "studies",
                            "id": "MGYS00000666",
                            "links": {
                                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/studies/MGYS00000666?format=api"
                            }
                        }
                    ]
                }
            },
            "links": {
                "self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372044?format=api"
            }
        }
    ],
    "meta": {
        "pagination": {
            "page": 1,
            "pages": 1,
            "count": 18
        }
    }
}