GET /metagenomics/api/v1/samples/SRS372823?format=api
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{
"data": {
"type": "samples",
"id": "SRS372823",
"attributes": {
"biosample": "SAMN01780144",
"longitude": null,
"latitude": null,
"sample-metadata": [
{
"key": "NCBI sample classification",
"value": "410658",
"unit": null
},
{
"key": "instrument model",
"value": "454 GS FLX Titanium",
"unit": null
}
],
"accession": "SRS372823",
"analysis-completed": "2016-04-14",
"collection-date": null,
"geo-loc-name": null,
"sample-desc": "Anguil sampling site (-36° 35' 31.56\", -63° 57' 31.05\") is located in an area with entic haplustolls soils. Mean annual precipitation and temperature are 760 mm and 15.3 ºC. Sampling was performed on September 2011 and wheat was recently planted in all plots under agricultural management. Three different plots under conventional tillage (CT) for the last 60 years and three different areas within one 12 ha plot under no-till farming (NT) during 4 years and more that 15 years under agricultural treatment were sampled. Rhizospheric soil from wheat plants on each NT and CT sampled spot was prepared. Plants and surrounding soil were taken with a shovel and sent to the lab where soil loosely attached to roots was disaggregated with gloved hands and finally soils attached to roots were obtained by brush scrapping. Soil samples were stored at -80°C until processing. Total DNA was obtained from all rhizospheric samples using PowerSoil DNA isolation kit from MoBio. Total RNA was obtained from NT and CT rhizospheric samples using PowerSoil Total RNA purification kit from MoBio. Single strand cDNA (cDNAss) was prepared from total RNA for further PCR amplification of pyrotags using first-strand cDNA synthesis system kit from Roche. Purification of prokaryotic mRNA was performed using MicrobeExpress kit from Ambion and double-stranded cDNA was prepared from mRNA using cDNA synthesis system kit from Roche. Pyrotags from gDNA and cDNA were generated by 16S rDNA V4 region amplification using F563-R802 primers developed by Ribosomal Database Project.",
"environment-biome": null,
"environment-feature": null,
"environment-material": null,
"sample-name": "Argentina Anguil Rhizospheric Soil: All sample amplicons from cDNA and gDNA",
"sample-alias": "Anguil Rhizosphere 16S Amplicons",
"host-tax-id": null,
"species": null,
"last-update": "2016-04-14T10:00:10"
},
"relationships": {
"studies": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372823/studies?format=api"
},
"data": [
{
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"id": "MGYS00000666",
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}
]
},
"biome": {
"data": {
"type": "biomes",
"id": "root:Environmental:Terrestrial:Soil"
},
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/biomes/root:Environmental:Terrestrial:Soil?format=api"
}
},
"runs": {
"links": {
"related": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372823/runs?format=api"
}
}
},
"links": {
"self": "https://www.ebi.ac.uk/metagenomics/api/v1/samples/SRS372823?format=api"
}
}
}