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Composite map of ciliary C2 central pair apparatus isolated from Chlamydomonas reinhardtii
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7som
Q-score: 0.397
Gui M, Wang X, Dutcher SK, Brown A, Zhang R
Nat Struct Mol Biol (2022) 29 pp. 483-492 [ DOI: doi:10.1038/s41594-022-00770-2 Pubmed: 35578023 ]
- Fap196 (111 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar wd repeat-containing protein pf20 (10 kDa, Protein from Chlamydomonas reinhardtii)
- Fap388 (6 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin alpha (49 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin beta (49 kDa, Protein from Chlamydomonas reinhardtii)
- Unknown protein (81 kDa, Protein from Chlamydomonas reinhardtii)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- C2 central pair apparatus complex (Complex from Chlamydomonas reinhardtii)
- Fap178 (65 kDa, Protein from Chlamydomonas reinhardtii)
- Unknown protein (22 kDa, Protein from Chlamydomonas reinhardtii)
- Fap65 (46 kDa, Protein from Chlamydomonas reinhardtii)
- Fap225 (23 kDa, Protein from Chlamydomonas reinhardtii)
- Fap70 (10 kDa, Protein from Chlamydomonas reinhardtii)
- Fap213 (102 kDa, Protein from Chlamydomonas reinhardtii)
- Fap239 (4 kDa, Protein from Chlamydomonas reinhardtii)
- Fap424 (4 kDa, Protein from Chlamydomonas reinhardtii)
- Fap147 (22 kDa, Protein from Chlamydomonas reinhardtii)
- Magnesium ion (24 Da, Ligand)
- Cilia- and flagella-associated protein 20 (64 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar associated protein (233 kDa, Protein from Chlamydomonas reinhardtii)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Unknown protein (57 kDa, Protein from Chlamydomonas reinhardtii)
Composite map of ciliary C1 central pair apparatus isolated from Chlamydomonas reinhardtii
Gui M, Wang X, Dutcher SK, Brown A, Zhang R
Nat Struct Mol Biol (2022) 29 pp. 483-492 [ DOI: doi:10.1038/s41594-022-00770-2 Pubmed: 35578023 ]
- Fap194 (57 kDa, Protein from Chlamydomonas reinhardtii)
- Fap275 (18 kDa, Protein from Chlamydomonas reinhardtii)
- Pf6 (237 kDa, Protein from Chlamydomonas reinhardtii)
- Fap221 (103 kDa, Protein from Chlamydomonas reinhardtii)
- Unknown protein (5 kDa, Protein from Chlamydomonas reinhardtii)
- Fap246 (120 kDa, Protein from Chlamydomonas reinhardtii)
- Fap42 (269 kDa, Protein from Chlamydomonas reinhardtii)
- Fap119 (33 kDa, Protein from Chlamydomonas reinhardtii)
- Fap47 (319 kDa, Protein from Chlamydomonas reinhardtii)
- Fap15 (34 kDa, Protein from Chlamydomonas reinhardtii)
- Fap99 (90 kDa, Protein from Chlamydomonas reinhardtii)
- Fap101 (85 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin alpha (49 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin beta (49 kDa, Protein from Chlamydomonas reinhardtii)
- C1 central pair apparatus complex (Complex from Chlamydomonas reinhardtii)
- Fap227 (19 kDa, Protein from Chlamydomonas reinhardtii)
- Hydin (528 kDa, Protein from Chlamydomonas reinhardtii)
- Fap74 (204 kDa, Protein from Chlamydomonas reinhardtii)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Fap54 (332 kDa, Protein from Chlamydomonas reinhardtii)
- Fap105 (31 kDa, Protein from Chlamydomonas reinhardtii)
- Fap7 (54 kDa, Protein from Chlamydomonas reinhardtii)
- Fap76 (172 kDa, Protein from Chlamydomonas reinhardtii)
- Pf16 (54 kDa, Protein from Chlamydomonas reinhardtii)
- Fap114 (31 kDa, Protein from Chlamydomonas reinhardtii)
- Fap81 (234 kDa, Protein from Chlamydomonas reinhardtii)
- Fap216 (79 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar associated protein (10 kDa, Protein from Chlamydomonas reinhardtii)
- Fap219 (29 kDa, Protein from Chlamydomonas smithii)
- Cpc1 (205 kDa, Protein from Chlamydomonas reinhardtii)
- Fap266 (81 kDa, Protein from Chlamydomonas reinhardtii)
- Calmodulin (18 kDa, Protein from Chlamydomonas reinhardtii)
- Unknown protein (3 kDa, Protein from Chlamydomonas reinhardtii)
- Unknown protein (4 kDa, Protein from Chlamydomonas reinhardtii)
- Phosphopyruvate hydratase (51 kDa, Protein from Chlamydomonas reinhardtii)
- Heat shock protein 70a (71 kDa, Protein from Chlamydomonas reinhardtii)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Fap305 (47 kDa, Protein from Chlamydomonas reinhardtii)
- Magnesium ion (24 Da, Ligand)
- Unknown protein (12 kDa, Protein from Chlamydomonas reinhardtii)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Fap69 (114 kDa, Protein from Chlamydomonas reinhardtii)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Fap297 (99 kDa, Protein from Chlamydomonas reinhardtii)
- Fap279 (43 kDa, Protein from Chlamydomonas reinhardtii)
- Fap108 (47 kDa, Protein from Chlamydomonas reinhardtii)
- Fap46 (289 kDa, Protein from Chlamydomonas reinhardtii)
Composite cryo-EM density map of the radial spoke 1 isolated from Chlamydomonas reinhardtii
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7jtk
Q-score: 0.501
Gui M, Ma M, Sze-Tu E, Wang X, Koh F, Zhong ED, Berger B, Davis JH, Dutcher SK, Zhang R, Brown A
Nat Struct Mol Biol (2021) 28 pp. 29-37 [ DOI: doi:10.1038/s41594-020-00530-0 Pubmed: 33318703 ]
- Phosphate ion (94 Da, Ligand)
- Flagellar radial spoke protein 4 (49 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 16 (39 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 3 (56 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 14 (40 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 2 (77 kDa, Protein from Chlamydomonas reinhardtii)
- Cytochrome b5 heme-binding domain-containing protein (26 kDa, Protein from Chlamydomonas reinhardtii)
- Uncharacterized protein (75 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 9 (29 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 11 (21 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 1 (87 kDa, Protein from Chlamydomonas reinhardtii)
- Predicted protein (8 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 10 (23 kDa, Protein from Chlamydomonas reinhardtii)
- Unknown protein (2 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 6 (48 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 12 (19 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 5 (55 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 7 (54 kDa, Protein from Chlamydomonas reinhardtii)
- Radial spoke 1 (Complex from Chlamydomonas reinhardtii)
- Dynein 8 kda light chain, flagellar outer arm (10 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar radial spoke protein 23 (61 kDa, Protein from Chlamydomonas reinhardtii)
Resolution: 6.1 Å
EM Method: Single-particle
Fitted PDBs: 7jts
Q-score: 0.268
Gui M, Ma M, Sze-Tu E, Wang X, Koh F, Zhong ED, Berger B, Davis JH, Dutcher SK, Zhang R, Brown A
Nat Struct Mol Biol (2021) 28 pp. 29-37 [ DOI: doi:10.1038/s41594-020-00530-0 Pubmed: 33318703 ]
- Fap207 (28 kDa, Protein from Chlamydomonas reinhardtii)
- Calmodulin (18 kDa, Protein from Chlamydomonas reinhardtii)
- Fap253 (75 kDa, Protein from Chlamydomonas reinhardtii)
- Stalk of radial spoke 1 (Complex from Chlamydomonas reinhardtii)
- Radial spoke protein 3 (56 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein 8 kda light chain, flagellar outer arm (10 kDa, Protein from Chlamydomonas reinhardtii)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ju4
Q-score: 0.431
Gui M, Ma M, Sze-Tu E, Wang X, Koh F, Zhong ED, Berger B, Davis JH, Dutcher SK, Zhang R, Brown A
Nat Struct Mol Biol (2021) 28 pp. 29-37 [ DOI: doi:10.1038/s41594-020-00530-0 Pubmed: 33318703 ]
- Complex of radial spoke 2 stalk, idac, and n-drc attached with doublet microtubule (Complex from Chlamydomonas reinhardtii)
- Dynein regulatory complex protein 1 (79 kDa, Protein from Chlamydomonas reinhardtii)
- Radial spoke protein 15 (35 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein regulatory complex subunit 4 (55 kDa, Protein from Chlamydomonas reinhardtii)
- Fap253 (75 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin alpha (49 kDa, Protein from Chlamydomonas reinhardtii)
- Radial spoke protein 7 (54 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin beta (49 kDa, Protein from Chlamydomonas reinhardtii)
- Radial spoke protein 3 (56 kDa, Protein from Chlamydomonas reinhardtii)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Radial spoke protein 11 (21 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein regulatory complex subunit 2 (64 kDa, Protein from Chlamydomonas reinhardtii)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Cfap91 domain-containing protein (134 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar-associated protein 59 (105 kDa, Protein from Chlamydomonas reinhardtii)
- Unknown protein (6 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar-associated protein 172 (101 kDa, Protein from Chlamydomonas reinhardtii)
- Radial spike protein 8 (40 kDa, Protein from Chlamydomonas reinhardtii)
- Fap207 (28 kDa, Protein from Chlamydomonas reinhardtii)
- Actin (41 kDa, Protein from Chlamydomonas reinhardtii)
- Magnesium ion (24 Da, Ligand)
- 28 kda inner dynein arm light chain, axonemal (28 kDa, Protein from Chlamydomonas reinhardtii)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Dynein 8 kda light chain, flagellar outer arm (10 kDa, Protein from Chlamydomonas reinhardtii)