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The structure of the large subunit of the mitoribosome from Neurospora crassa
Release date: Nov. 11, 2020
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 6yws
Q-score: 0.601
Resolution: 2.74 Å
EM Method: Single-particle
Fitted PDBs: 6yws
Q-score: 0.601
Itoh Y, Naschberger A, Mortezaei N, Herrmann JM, Amunts A
Nat Commun (2020) 11 pp. 5187-5187 [ Pubmed: 33056988 DOI: doi:10.1038/s41467-020-18830-w ]
Sample:
- Mitochondrial ribosomal protein l43 (15 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein subunit l32 (14 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (49 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein subunit l23 (26 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l6 (28 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (11 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal protein l17 (22 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (23 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l20 (25 kDa, Protein from Neurospora crassa OR74A)
- Rnase iii domain-containing protein (29 kDa, Protein from Neurospora crassa OR74A)
- 54s ribosomal protein l4, mitochondrial (29 kDa, Protein from Neurospora crassa OR74A)
- Ribosomal protein (13 kDa, Protein from Neurospora crassa OR74A)
- Mitoc_ml59 domain-containing protein (18 kDa, Protein from Neurospora crassa OR74A)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- 50s ribosomal protein l30 (18 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (37 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial large ribosomal subunit yml35 (50 kDa, Protein from Neurospora crassa OR74A)
- Spermine (202 Da, Ligand)
- Uncharacterized protein (18 kDa, Protein from Neurospora crassa OR74A)
- Kow domain-containing protein (45 kDa, Protein from Neurospora crassa OR74A)
- Potassium ion (39 Da, Ligand)
- Uncharacterized protein (7 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal protein l24 (31 kDa, Protein from Neurospora crassa OR74A)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l16 (27 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l3 (41 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein (28 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l1 (33 kDa, Protein from Neurospora crassa OR74A)
- Aconitate hydratase (23 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (41 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial large ribosomal subunit (41 kDa, Protein from Neurospora crassa OR74A)
- 54s ribosomal protein l31, mitochondrial (13 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (23 kDa, Protein from Neurospora crassa OR74A)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l5 (39 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l2 (42 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal subunit l30 (41 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal protein l14 (14 kDa, Protein from Neurospora crassa OR74A)
- Ribosomal protein l13 (20 kDa, Protein from Neurospora crassa OR74A)
- Mitoribosome of neurospora crassa (Complex from Neurospora crassa OR74A)
- 60s ribosomal protein l4, variant (36 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l19 (16 kDa, Protein from Neurospora crassa OR74A)
- Ribosomal_l9 domain-containing protein (33 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein l44 (11 kDa, Protein from Neurospora crassa OR74A)
- Ribosomal protein l15 (33 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (32 kDa, Protein from Neurospora crassa OR74A)
- L51_s25_ci-b8 domain-containing protein (22 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (49 kDa, Protein from Neurospora crassa OR74A)
- Related to ribosomal protein l34, mitochondrial (14 kDa, Protein from Neurospora crassa OR74A)
- Rna (2820-mer) (1 MDa, RNA from Neurospora crassa OR74A)
- 60s ribosomal protein l3 (48 kDa, Protein from Neurospora crassa OR74A)
The structure of the Atp25 bound assembly intermediate of the mitoribosome from Neurospora crassa
Release date: Nov. 11, 2020
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 6ywv
Q-score: 0.544
Resolution: 3.03 Å
EM Method: Single-particle
Fitted PDBs: 6ywv
Q-score: 0.544
Itoh Y, Naschberger A, Mortezaei N, Herrmann JM, Amunts A
Nat Commun (2020) 11 pp. 5187-5187 [ Pubmed: 33056988 DOI: doi:10.1038/s41467-020-18830-w ]
Sample:
- Mitochondrial ribosomal protein l43 (15 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein subunit l32 (14 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (49 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein subunit l23 (26 kDa, Protein from Neurospora crassa OR74A)
- Assembly intermediate of the mitoribosome of neurospora crassa (Complex from Neurospora crassa OR74A)
- 60s ribosomal protein l6 (28 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (14 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (11 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal protein l17 (22 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (23 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l20 (25 kDa, Protein from Neurospora crassa OR74A)
- Rnase iii domain-containing protein (29 kDa, Protein from Neurospora crassa OR74A)
- 54s ribosomal protein l4, mitochondrial (29 kDa, Protein from Neurospora crassa OR74A)
- 23 s rrna (1 MDa, RNA from Neurospora crassa OR74A)
- Ribosomal protein (13 kDa, Protein from Neurospora crassa OR74A)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- 50s ribosomal subunit protein l15 (33 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal protein l30 (18 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (37 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial large ribosomal subunit yml35 (50 kDa, Protein from Neurospora crassa OR74A)
- Spermine (202 Da, Ligand)
- Uncharacterized protein (18 kDa, Protein from Neurospora crassa OR74A)
- Kow domain-containing protein (45 kDa, Protein from Neurospora crassa OR74A)
- Potassium ion (39 Da, Ligand)
- Uncharacterized protein (7 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal protein l24 (31 kDa, Protein from Neurospora crassa OR74A)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l16 (27 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l3 (41 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein (28 kDa, Protein from Neurospora crassa OR74A)
- Atpase synthesis protein 25, mitochondrial (77 kDa, Protein from Neurospora crassa OR74A)
- Aconitate hydratase (23 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (41 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial large ribosomal subunit (41 kDa, Protein from Neurospora crassa OR74A)
- 54s ribosomal protein l31, mitochondrial (13 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (23 kDa, Protein from Neurospora crassa OR74A)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l5 (39 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l2 (42 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal subunit l30 (41 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal protein l14 (14 kDa, Protein from Neurospora crassa OR74A)
- Ribosomal protein l13 (20 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l4, variant (36 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l19 (16 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein l44 (11 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (32 kDa, Protein from Neurospora crassa OR74A)
- Uncharacterized protein (49 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l3 (48 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial 60s ribosomal protein l25 (18 kDa, Protein from Neurospora crassa OR74A)
The structure of the mitoribosome from Neurospora crassa with tRNA bound to the E-site
Release date: Nov. 11, 2020
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6ywx
Q-score: 0.546
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 6ywx
Q-score: 0.546
Itoh Y, Naschberger A, Mortezaei N, Herrmann JM, Amunts A
Nat Commun (2020) 11 pp. 5187-5187 [ Pubmed: 33056988 DOI: doi:10.1038/s41467-020-18830-w ]
Sample:
- Mitochondrial ribosomal protein subunit l23 (26 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l6 (28 kDa, Protein from Neurospora crassa OR74A)
- Ml50 (49 kDa, Protein from Neurospora crassa OR74A)
- 54s ribosomal protein l4, mitochondrial (29 kDa, Protein from Neurospora crassa OR74A)
- Ribosomal protein (13 kDa, Protein from Neurospora crassa OR74A)
- Ms45 (39 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal subunit protein l15 (33 kDa, Protein from Neurospora crassa OR74A)
- Ribosomal_s7 domain-containing protein (33 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l16 (27 kDa, Protein from Neurospora crassa OR74A)
- Bl27m (49 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l1 (33 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial large ribosomal subunit (41 kDa, Protein from Neurospora crassa OR74A)
- Ms33 (12 kDa, Protein from Neurospora crassa OR74A)
- Mito ribosomal protein s6 (13 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l4, variant (36 kDa, Protein from Neurospora crassa OR74A)
- 37s ribosomal protein s24, mitochondrial (44 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial 60s ribosomal protein l25 (18 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial 37s ribosomal protein s17 (18 kDa, Protein from Neurospora crassa OR74A)
- Ms26 (35 kDa, Protein from Neurospora crassa OR74A)
- Ml41 (11 kDa, Protein from Neurospora crassa OR74A)
- 37s ribosomal protein s10, mitochondrial (30 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal protein l17 (22 kDa, Protein from Neurospora crassa OR74A)
- Ribosomal protein s15 (35 kDa, Protein from Neurospora crassa OR74A)
- Trna e-site (19 kDa, RNA from Neurospora crassa OR74A)
- Ribosomal protein s16, mitochondrial (12 kDa, Protein from Neurospora crassa OR74A)
- Potassium ion (39 Da, Ligand)
- 37s ribosomal protein s25, mitochondrial (29 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l20 (25 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein dap3 (51 kDa, Protein from Neurospora crassa OR74A)
- Ml49 (18 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein s19 (10 kDa, Protein from Neurospora crassa OR74A)
- Mito ribosomal protein s18 (28 kDa, Protein from Neurospora crassa OR74A)
- 37s ribosomal protein mrp10, mitochondrial (9 kDa, Protein from Neurospora crassa OR74A)
- Ul10m (37 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l19 (16 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal protein l30 (18 kDa, Protein from Neurospora crassa OR74A)
- Aconitate hydratase (23 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial 40s ribosomal protein mrp2 (13 kDa, Protein from Neurospora crassa OR74A)
- 40s ribosomal protein s13 (13 kDa, Protein from Neurospora crassa OR74A)
- Ml67 (32 kDa, Protein from Neurospora crassa OR74A)
- 37s ribosomal protein s9, mitochondrial (35 kDa, Protein from Neurospora crassa OR74A)
- Duf1713 domain-containing protein (38 kDa, Protein from Neurospora crassa OR74A)
- 3-hydroxyisobutyryl-coa hydrolase, mitochondrial (56 kDa, Protein from Neurospora crassa OR74A)
- Igr domain-containing protein (26 kDa, Protein from Neurospora crassa OR74A)
- Mitoribosome of neurospora crassa with trna in the e-site (Complex from Neurospora crassa OR74A)
- Mito ribosomal protein s21 (25 kDa, Protein from Neurospora crassa OR74A)
- 23s rrna (1 MDa, RNA from Neurospora crassa OR74A)
- Mito ribosomal protein s4 (51 kDa, Protein from Neurospora crassa OR74A)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal subunit l30 (41 kDa, Protein from Neurospora crassa OR74A)
- Ml54 (23 kDa, Protein from Neurospora crassa OR74A)
- Bs1m (51 kDa, Protein from Neurospora crassa OR74A)
- If1 (10 kDa, Protein from Neurospora crassa OR74A)
- Ribosomal_l9 domain-containing protein (33 kDa, Protein from Neurospora crassa OR74A)
- Kow domain-containing protein (45 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein l44 (11 kDa, Protein from Neurospora crassa OR74A)
- Mito ribosomal protein s2 (47 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein subunit s18 (42 kDa, Protein from Neurospora crassa OR74A)
- Ml40 (41 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein subunit l32 (14 kDa, Protein from Neurospora crassa OR74A)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- 50s ribosomal protein l14 (14 kDa, Protein from Neurospora crassa OR74A)
- 40s ribosomal protein s8 (17 kDa, Protein from Neurospora crassa OR74A)
- Rnase iii domain-containing protein (29 kDa, Protein from Neurospora crassa OR74A)
- Bl33m (7 kDa, Protein from Neurospora crassa OR74A)
- 37s ribosomal protein s5 (53 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial large ribosomal subunit yml35 (50 kDa, Protein from Neurospora crassa OR74A)
- Bl31m (23 kDa, Protein from Neurospora crassa OR74A)
- Spermine (202 Da, Ligand)
- 50s ribosomal protein l24 (31 kDa, Protein from Neurospora crassa OR74A)
- Zinc ion (65 Da, Ligand)
- 60s ribosomal protein l3 (41 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein (28 kDa, Protein from Neurospora crassa OR74A)
- Ms27 (44 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial ribosomal protein l43 (15 kDa, Protein from Neurospora crassa OR74A)
- Ms46 (43 kDa, Protein from Neurospora crassa OR74A)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Ribosomal protein s5, mitochondrial (58 kDa, Protein from Neurospora crassa OR74A)
- 54s ribosomal protein l31, mitochondrial (13 kDa, Protein from Neurospora crassa OR74A)
- 16s rrna (600 kDa, RNA from Neurospora crassa OR74A)
- Fe superoxide dismutase (34 kDa, Protein from Neurospora crassa OR74A)
- 50s ribosomal protein l5 (39 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l2 (42 kDa, Protein from Neurospora crassa OR74A)
- Ribosomal protein l13 (20 kDa, Protein from Neurospora crassa OR74A)
- L51_s25_ci-b8 domain-containing protein (22 kDa, Protein from Neurospora crassa OR74A)
- Mitochondrial 30s ribosomal protein s12 (18 kDa, Protein from Neurospora crassa OR74A)
- Related to ribosomal protein l34, mitochondrial (14 kDa, Protein from Neurospora crassa OR74A)
- 60s ribosomal protein l3 (48 kDa, Protein from Neurospora crassa OR74A)
Structure of human mitochondrial 28S ribosome in complex with mitochondrial IF3
Release date: June 3, 2020
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 6rw4
Q-score: 0.599
Resolution: 2.97 Å
EM Method: Single-particle
Fitted PDBs: 6rw4
Q-score: 0.599
Khawaja A, Itoh Y, Remes C, Spahr H, Yukhnovets O, Hofig H, Amunts A, Rorbach J
Nat Commun (2020) 11 pp. 2932-2932 [ DOI: doi:10.1038/s41467-020-16503-2 Pubmed: 32522994 ]
Sample:
- 28s ribosomal protein s34, mitochondrial (25 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s27, mitochondrial (47 kDa, Protein from Homo sapiens)
- Ribosome (Complex from Homo sapiens)
- 28s ribosomal protein s9, mitochondrial (45 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s31, mitochondrial (45 kDa, Protein from Homo sapiens)
- If3 bound mitochondrial translation pre-initiation complex (1 MDa, Complex)
- 28s ribosomal protein s11, mitochondrial (20 kDa, Protein from Homo sapiens)
- If3 (Complex from Homo sapiens)
- 28s ribosomal protein s12, mitochondrial (15 kDa, Protein from Homo sapiens)
- Nicotinamide-adenine-dinucleotide (663 Da, Ligand)
- 28s ribosomal protein s6, mitochondrial (14 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s26, mitochondrial (24 kDa, Protein from Homo sapiens)
- Pentatricopeptide repeat domain-containing protein 3, mitochondrial (78 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s29, mitochondrial (45 kDa, Protein from Homo sapiens)
- Spermine (202 Da, Ligand)
- 28s ribosomal protein s23, mitochondrial (21 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
- 28s ribosomal protein s17, mitochondrial (14 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s10, mitochondrial (23 kDa, Protein from Homo sapiens)
- Streptomycin (581 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Aurora kinase a-interacting protein (22 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s33, mitochondrial (12 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s25, mitochondrial (20 kDa, Protein from Homo sapiens)
- Coiled-coil-helix-coiled-coil-helix domain-containing protein 1 (13 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 12s mitochondrial rrna (306 kDa, RNA from Homo sapiens)
- 28s ribosomal protein s5, mitochondrial (48 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s2, mitochondrial (33 kDa, Protein from Homo sapiens)
- Water (18 Da, Ligand)
- Translation initiation factor if-3, mitochondrial (32 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s24, mitochondrial (19 kDa, Protein from Homo sapiens)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- 28s ribosomal protein s35, mitochondrial (36 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 28s ribosomal protein s28, mitochondrial (20 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s7, mitochondrial (28 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s16, mitochondrial (15 kDa, Protein from Homo sapiens)
- Fe2/s2 (inorganic) cluster (175 Da, Ligand)
- 28s ribosomal protein s15, mitochondrial (29 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s22, mitochondrial (41 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s14, mitochondrial (15 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s18b, mitochondrial (29 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s18c, mitochondrial (15 kDa, Protein from Homo sapiens)
- 28s ribosomal protein s21, mitochondrial (10 kDa, Protein from Homo sapiens)