Yan L, Yang Y, Li M, Zhang Y, Zheng L, Ge J, Huang YC, Liu Z, Wang T, Gao S, Zhang R, Huang YY, Guddat LW, Gao Y, Rao Z, Lou Z
Cell (2021) 184 pp. 3474-3485.e11 [ Pubmed: 34143953 DOI: doi:10.1016/j.cell.2021.05.033 ]
- Primer rna, template rna (Complex)
- Non-structural protein 9 (12 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 8 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Nsp 7, 8, 9, 10, 12, 13, 14 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Helicase (66 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Mcap(0)-rtc (Complex from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Non-structural protein 10 (14 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Proofreading exoribonuclease (59 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Primer (8 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Template (8 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Cryo-EM structure of SARS-CoV-2 nsp10-nsp14 (WT)-RNA complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 7n0b
Q-score: 0.38
Liu C, Shi W, Becker ST, Schatz DG, Liu B, Yang Y
Science (2021) 373 pp. 1142-1146 [ Pubmed: 34315827 DOI: doi:10.1126/science.abi9310 ]
- Rna (5'-r(*ap*gp*ap*ap*gp*cp*up*ap*up*up*ap*ap*ap*ap*up*cp*ap*cp*c)-3') (10 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Water (18 Da, Ligand)
- Non-structural protein 10 (14 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Sars-cov-2 nsp10-nsp14 (wt)-rna complex (Complex from Severe acute respiratory syndrome coronavirus 2)
- Calcium ion (40 Da, Ligand)
- Proofreading exoribonuclease (59 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(*ap*up*gp*up*gp*ap*up*up*up*up*ap*ap*up*ap*gp*cp*up*up*cp*u)-3') (12 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Cryo-EM structure of the monomeric form of SARS-CoV-2 nsp10-nsp14 (E191A)-RNA complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7n0c
Q-score: 0.471
Liu C, Shi W, Becker ST, Schatz DG, Liu B, Yang Y
Science (2021) 373 pp. 1142-1146 [ Pubmed: 34315827 DOI: doi:10.1126/science.abi9310 ]
- Monomeric form of sars-cov-2 nsp10-nsp14 (e191a)-rna complex (Complex from Severe acute respiratory syndrome coronavirus 2)
- Proofreading exoribonuclease (59 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (25-mer) (11 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Rna (24-mer) (10 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Water (18 Da, Ligand)
- Non-structural protein 10 (14 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of the tetrameric form of SARS-CoV-2 nsp10-nsp14 (E191A)-RNA complex
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7n0d
Q-score: 0.542
Liu C, Shi W, Becker ST, Schatz DG, Liu B, Yang Y
Science (2021) 373 pp. 1142-1146 [ Pubmed: 34315827 DOI: doi:10.1126/science.abi9310 ]
- Proofreading exoribonuclease (59 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(*gp*gp*gp*gp*ap*up*gp*up*gp*ap*up*up*up*up*ap*ap*up*ap*g)-3') (8 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Rna (5'-r(*cp*cp*cp*cp*c)-3') (1 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Chapso (631 Da, Ligand)
- Water (18 Da, Ligand)
- Non-structural protein 10 (14 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Tetrameric form of sars-cov-2 nsp10-nsp14 (e191a)-rna complex (Complex from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(*cp*up*ap*up*up*ap*ap*ap*ap*up*cp*ap*cp*c)-3') (7 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
Resolution: 3.35 Å
EM Method: Single-particle
Fitted PDBs: 7egq
Q-score: 0.384
Yan L, Yang Y, Li M, Zhang Y, Zheng L, Ge J, Huang YC, Liu Z, Wang T, Gao S, Zhang R, Huang YY, Guddat LW, Gao Y, Rao Z, Lou Z
Cell (2021) 184 pp. 3474-3485.e11 [ Pubmed: 34143953 DOI: doi:10.1016/j.cell.2021.05.033 ]
- Primer rna, template rna (Complex)
- Proofreading exoribonuclease (59 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 8 (21 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Nsp 7, 8, 9, 10, 12, 13, 14 (Complex from Severe acute respiratory syndrome coronavirus 2)
- Helicase (66 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Primer rna (8 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Rna-directed rna polymerase (106 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 10 (14 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Cap(0)-rtc (Complex from Severe acute respiratory syndrome coronavirus 2)
- Non-structural protein 7 (9 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Template rna (10 kDa, RNA from Severe acute respiratory syndrome coronavirus 2)
- Magnesium ion (24 Da, Ligand)
- Non-structural protein 9 (12 kDa, Protein from Severe acute respiratory syndrome coronavirus 2)
- Zinc ion (65 Da, Ligand)