Cryo-EM Structure of Pre-B+5'ss+ATPgammaS Complex (core part)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8qoz
Q-score: 0.519
Zhang Z, Kumar V, Dybkov O, Will CL, Zhong J, Ludwig SEJ, Urlaub H, Kastner B, Stark H, Luhrmann R
Nature (2024) [ Pubmed: 38778104 DOI: doi:10.1038/s41586-024-07458-1 ]
- Human spliceosomal pre-b+5'ss+atpys complex (Complex from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (18 kDa, Protein from Homo sapiens)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
- U4 snrna (46 kDa, RNA from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1, n-terminally processed (14 kDa, Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 39 (65 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- 5'ss rna oligo (3 kDa, RNA from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
Cryo-EM Structure of Pre-B Complex (core part)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8qp8
Q-score: 0.441
Zhang Z, Kumar V, Dybkov O, Will CL, Zhong J, Ludwig SEJ, Urlaub H, Kastner B, Stark H, Luhrmann R
Nature (2024) [ Pubmed: 38778104 DOI: doi:10.1038/s41586-024-07458-1 ]
- U4 snrna (46 kDa, RNA from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 39 (65 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Human spliceosomal cross-exon pre-b complex (Complex from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- U4/u6.u5 small nuclear ribonucleoprotein 27 kda protein (18 kDa, Protein from Homo sapiens)
- Rna-binding protein 42 (50 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
Cryo-EM Structure of Pre-B+AMPPNP Complex (core part)
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8qp9
Q-score: 0.408
Zhang Z, Kumar V, Dybkov O, Will CL, Zhong J, Ludwig SEJ, Urlaub H, Kastner B, Stark H, Luhrmann R
Nature (2024) [ Pubmed: 38778104 DOI: doi:10.1038/s41586-024-07458-1 ]
- U4 snrna (46 kDa, RNA from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Human spliceosomal pre-b+amppnp complex (Complex from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 39 (65 kDa, Protein from Homo sapiens)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- U4/u6.u5 small nuclear ribonucleoprotein 27 kda protein (18 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Rna-binding protein 42 (50 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
Cryo-EM Structure of Pre-B+5'ssLNG Complex (core part)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8qpa
Q-score: 0.439
Zhang Z, Kumar V, Dybkov O, Will CL, Zhong J, Ludwig SEJ, Urlaub H, Kastner B, Stark H, Luhrmann R
Nature (2024) [ Pubmed: 38778104 DOI: doi:10.1038/s41586-024-07458-1 ]
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
- U4 snrna (46 kDa, RNA from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- 5'ss oligo (5 kDa, RNA from Homo sapiens)
- Human spliceosomal pre-b+5'sslng+atpys complex (Complex from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1, n-terminally processed (14 kDa, Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 39 (65 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
Cryo-EM Structure of Pre-B+ATP Complex (core part)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8qpb
Q-score: 0.423
Zhang Z, Kumar V, Dybkov O, Will CL, Zhong J, Ludwig SEJ, Urlaub H, Kastner B, Stark H, Luhrmann R
Nature (2024) [ Pubmed: 38778104 DOI: doi:10.1038/s41586-024-07458-1 ]
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
- U4 snrna (46 kDa, RNA from Homo sapiens)
- Human spliceosomal pre-b+atp complex (Complex from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1, n-terminally processed (14 kDa, Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 39 (65 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- 5'ss oligo (4 kDa, RNA from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
Cryo-EM Structure of Pre-B-like Complex (core part)
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 8qpe
Q-score: 0.495
Zhang Z, Kumar V, Dybkov O, Will CL, Zhong J, Ludwig SEJ, Urlaub H, Kastner B, Stark H, Luhrmann R
Nature (2024) [ Pubmed: 38778104 DOI: doi:10.1038/s41586-024-07458-1 ]
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Homo sapiens)
- Oligo2 (1 kDa, RNA from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
- U4 snrna (46 kDa, RNA from Homo sapiens)
- Human spliceosomal cross-exon b-like complex (Complex from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Ww domain-binding protein 4 (42 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1, n-terminally processed (14 kDa, Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 39 (65 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
- Oligo1 (3 kDa, RNA from Homo sapiens)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
Cryo-EM Structure of Pre-B+5'ss Complex (core part)
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 8qpk
Q-score: 0.365
Zhang Z, Kumar V, Dybkov O, Will CL, Zhong J, Ludwig SEJ, Urlaub H, Kastner B, Stark H, Luhrmann R
Nature (2024) [ Pubmed: 38778104 DOI: doi:10.1038/s41586-024-07458-1 ]
- U4 snrna (46 kDa, RNA from Homo sapiens)
- Inositol hexakisphosphate (660 Da, Ligand)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 39 (65 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- U4/u6.u5 small nuclear ribonucleoprotein 27 kda protein (18 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Human spliceosomal pre-b+5'ss complex (Complex from Homo sapiens)
- 5'ss oligo (3 kDa, RNA from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Rna-binding protein 42 (50 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
Cryo-EM structure of the cross-exon pre-B complex
Resolution: 9.6 Å
EM Method: Single-particle
Fitted PDBs: 8qxd
Q-score: 0.096
Zhang Z, Kumar V, Dybkov O, Will CL, Zhong J, Ludwig SEJ, Urlaub H, Kastner B, Stark H, Luhrmann R
Nature (2024) [ Pubmed: 38778104 DOI: doi:10.1038/s41586-024-07458-1 ]
- U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- Rna-binding protein 42 (50 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- U6 snrna (24 kDa, RNA from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
- U4 snrna (46 kDa, RNA from Homo sapiens)
- U5 snrna (36 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (9 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- U2 snrna (58 kDa, RNA from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1, n-terminally processed (14 kDa, Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 39 (65 kDa, Protein from Homo sapiens)
- U4/u6.u5 small nuclear ribonucleoprotein 27 kda protein (18 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (9 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (9 kDa, Protein from Homo sapiens)
- Pre-mrna (4 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- Cross-exon pre-b complex (Complex from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- Serine/threonine-protein kinase prp4 homolog (117 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Homo sapiens)
Cryo-EM structure of the cross-exon B-like complex
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8qzs
Q-score: 0.168
Zhang Z, Kumar V, Dybkov O, Will CL, Zhong J, Ludwig SEJ, Urlaub H, Kastner B, Stark H, Luhrmann R
Nature (2024) [ Pubmed: 38778104 DOI: doi:10.1038/s41586-024-07458-1 ]
- U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- Oligo 2 (1 kDa, RNA from Homo sapiens)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- Pre-mrna-splicing factor 38a (37 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
- Pre-mrna (4 kDa, RNA from Homo sapiens)
- U4 snrna (46 kDa, RNA from Homo sapiens)
- U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Homo sapiens)
- Peptidyl-prolyl cis-trans isomerase h (19 kDa, Protein from Homo sapiens)
- Human spliceosomal cross-exon b-like complex (Complex from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Oligo 1 (3 kDa, RNA from Homo sapiens)
- Ww domain-binding protein 4 (42 kDa, Protein from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1, n-terminally processed (14 kDa, Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 39 (65 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- Protein red (65 kDa, Protein from Homo sapiens)
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Zinc finger matrin-type protein 2 (23 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Microfibrillar-associated protein 1 (52 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- Wd40 repeat-containing protein smu1 (57 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Homo sapiens)
Cryo-EM structure of the cross-exon pre-B+AMPPNP complex
Resolution: 6.1 Å
EM Method: Single-particle
Fitted PDBs: 8r08
Q-score: 0.133
Zhang Z, Kumar V, Dybkov O, Will CL, Zhong J, Ludwig SEJ, Urlaub H, Kastner B, Stark H, Luhrmann R
Nature (2024) [ Pubmed: 38778104 DOI: doi:10.1038/s41586-024-07458-1 ]
- U2 snrna (60 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm5 (9 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Homo sapiens)
- U4/u6.u5 tri-snrnp-associated protein 1 (90 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm4 (15 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein-associated proteins b and b' (24 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Homo sapiens)
- Rna-binding protein 42 (50 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Homo sapiens)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm2 (10 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Homo sapiens)
- Thioredoxin-like protein 4a (16 kDa, Protein from Homo sapiens)
- U4 snrna (46 kDa, RNA from Homo sapiens)
- U6 snrna-associated sm-like protein lsm3 (11 kDa, Protein from Homo sapiens)
- U1 snrna (52 kDa, RNA from Homo sapiens)
- U6 snrna (34 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Homo sapiens)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm6 (9 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp31 (55 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1, n-terminally processed (14 kDa, Protein from Homo sapiens)
- Ubiquitin carboxyl-terminal hydrolase 39 (65 kDa, Protein from Homo sapiens)
- U4/u6.u5 small nuclear ribonucleoprotein 27 kda protein (18 kDa, Protein from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp4 (58 kDa, Protein from Homo sapiens)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Homo sapiens)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Homo sapiens)
- U1 small nuclear ribonucleoprotein 70 kda (51 kDa, Protein from Homo sapiens)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Homo sapiens)
- Oligo 1 (1 kDa, RNA from Homo sapiens)
- Pre-mrna (4 kDa, RNA from Homo sapiens)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Homo sapiens)
- Human spliceosomal pre-b+amppnp complex (Complex from Homo sapiens)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Homo sapiens)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Homo sapiens)
- Probable atp-dependent rna helicase ddx23 (95 kDa, Protein from Homo sapiens)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Homo sapiens)
- Pre-mrna-processing factor 6 (107 kDa, Protein from Homo sapiens)
- Serine/threonine-protein kinase prp4 homolog (117 kDa, Protein from Homo sapiens)
- U5 snrna (37 kDa, RNA from Homo sapiens)
- U4/u6 small nuclear ribonucleoprotein prp3 (77 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm8 (10 kDa, Protein from Homo sapiens)
- U6 snrna-associated sm-like protein lsm7 (11 kDa, Protein from Homo sapiens)