Cryo-EM structure of H. thermophilus GroEL-GroES2 asymmetric football complex
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8wuw
Q-score: 0.444
Liao Z, Gopalasingam CC, Kameya M, Gerle C, Shigematsu H, Ishii M, Arakawa T, Fushinobu S
Structure (2024) 32 pp. 679-689.e4 [ Pubmed: 38492570 DOI: doi:10.1016/j.str.2024.02.012 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Chaperonin groel (56 kDa, Protein from Hydrogenobacter thermophilus TK-6)
- Co-chaperonin groes (Complex)
- Chaperonin groel (Complex)
- H. thermophilus groel-groes2 asymmetric football complex (Complex from Hydrogenobacter thermophilus TK-6)
- Potassium ion (39 Da, Ligand)
- Co-chaperonin groes (10 kDa, Protein from Hydrogenobacter thermophilus TK-6)
Cryo-EM structure of H. thermophilus GroEL-GroES bullet complex
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 8wux
Q-score: 0.453
Liao Z, Gopalasingam CC, Kameya M, Gerle C, Shigematsu H, Ishii M, Arakawa T, Fushinobu S
Structure (2024) 32 pp. 679-689.e4 [ Pubmed: 38492570 DOI: doi:10.1016/j.str.2024.02.012 ]
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Chaperonin groel (56 kDa, Protein from Hydrogenobacter thermophilus TK-6)
- Co-chaperonin groes (10 kDa, Protein from Hydrogenobacter thermophilus TK-6)
- Chaperonin groel (Complex)
- H. thermophilus groel-groes bullet complex (Complex from Hydrogenobacter thermophilus TK-6)
- Potassium ion (39 Da, Ligand)
- Co-chaperonin groes (Complex)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 8g7p
Q-score: 0.557
Nat Struct Mol Biol (2024) 31 pp. 810-816 [ Pubmed: 38538914 DOI: doi:10.1038/s41594-024-01236-3 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- M-i mrna (8 kDa, RNA from Escherichia phage T4)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- Isoleucine (131 Da, Ligand)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- Elongation factor tu (43 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- Structure of the escherichia coli 70s ribosome in complex with ef-tu and ile-trnaile(lau) bound to the cognate aua codon (structure i) (2 MDa, Complex from Escherichia coli)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- Isoleucine trna (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
- P-site initiator trna (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- Phosphomethylphosphonic acid guanylate ester (521 Da, Ligand)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8g7r
Q-score: 0.534
Nat Struct Mol Biol (2024) 31 pp. 810-816 [ Pubmed: 38538914 DOI: doi:10.1038/s41594-024-01236-3 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- M-i mrna (8 kDa, RNA from Escherichia phage T4)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- Ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- Isoleucine (131 Da, Ligand)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 16s ribosomal rna (499 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- Isoleucine trna (24 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
- Structure of the escherichia coli 70s ribosome in complex with a-site trnaile(lau) bound to the cognate aua codon (structure iii) (2 MDa, Complex from Escherichia coli)
- 30s ribosomal protein s7 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- P-site initiator trna (24 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- Paromomycin (615 Da, Ligand)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
GroEL:GroES-ATP complex under continuous turnover condirions
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8bkz
Q-score: 0.632
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Hetero 14mer assembled from 2 heptameric rings (950 kDa, Complex from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Water (18 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Co-chaperonin groes (10 kDa, Protein from Escherichia coli)
- Chaperonin groel (57 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
Structure of GroEL-ATP complex plunge frozen 200 ms after reaction initiation
Resolution: 2.3 Å
EM Method: Single-particle
Fitted PDBs: 8bl2
Q-score: 0.465
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Groel in complex with atp (800 kDa, Complex from Escherichia coli)
- Water (18 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Chaperonin groel (57 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
Structure of the GroEL-ATP complex plunge-frozen 50 ms after mixing with ATP
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8blc
Q-score: 0.462
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Groel in complex with atp (800 kDa, Complex from Escherichia coli)
- Water (18 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Chaperonin groel (57 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
Structure of GroEL:GroES-ATP complex plunge frozen 200 ms after reaction initiation
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8bm0
Q-score: 0.401
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Co-chaperonin groes (10 kDa, Protein from Escherichia coli)
- Groel:groes-atp complex (950 kDa, Complex from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- Chaperonin groel (57 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
Structure of GroEL:GroES-ATP complex under continuous turnover conditions
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 8bm1
Q-score: 0.518
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Hetero 14mer assembled from 2 heptameric rings (950 kDa, Complex from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Co-chaperonin groes (10 kDa, Protein from Escherichia coli)
- Water (18 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Chaperonin groel (57 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
Structure of GroEL-ATP complex under continuous turnover conditions
Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8bmd
Q-score: 0.434
Torino S, Dhurandhar M, Stroobants A, Claessens R, Efremov RG
Nat Methods (2023) 20 pp. 1400-1408 [ Pubmed: 37592181 DOI: doi:10.1038/s41592-023-01967-z ]
- Groel in complex with atp under continuous turnover of atp (800 kDa, Complex from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Water (18 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Chaperonin groel (57 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)