Retapamulin and Capreomycin bound to the 50S subunit
Resolution: 1.83 Å
EM Method: Single-particle
Fitted PDBs: 8ceu
Q-score: 0.756
Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmuller H, Novacek J, Wilson DN
Nat Struct Mol Biol (2023) 30 pp. 1380-1392 [ DOI: doi:10.1038/s41594-023-01047-y Pubmed: 37550453 ]
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl33 (6 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl28 (9 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli BW25113)
- 23s rrna (941 kDa, RNA from Escherichia coli BW25113)
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl27 (9 kDa, Protein from Escherichia coli BW25113)
- Potassium ion (39 Da, Ligand)
- Large ribosomal subunit protein ul23 (11 kDa, Protein from Escherichia coli BW25113)
- Zinc ion (65 Da, Ligand)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli BW25113)
- 70s ribosomes with antibiotic cocktail (Complex from Escherichia coli BW25113)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli BW25113)
- Capreomycin ia (686 Da, Protein from Streptomyces californicus)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli BW25113)
- Water (18 Da, Ligand)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli BW25113)
- Magnesium ion (24 Da, Ligand)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli BW25113)
- Retapamulin (517 Da, Ligand)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli BW25113)
- 5s rrna (38 kDa, RNA from Escherichia coli BW25113)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl17 (14 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli BW25113)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli BW25113)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli BW25113)
Kasugamycin bound to the 30S body
Resolution: 2.04 Å
EM Method: Single-particle
Fitted PDBs: 8cep
Q-score: 0.734
Paternoga H, Crowe-McAuliffe C, Bock LV, Koller TO, Morici M, Beckert B, Myasnikov AG, Grubmuller H, Novacek J, Wilson DN
Nat Struct Mol Biol (2023) 30 pp. 1380-1392 [ DOI: doi:10.1038/s41594-023-01047-y Pubmed: 37550453 ]
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us15 (10 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us12 (13 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us5 (17 kDa, Protein from Escherichia coli BW25113)
- 23s rrna (941 kDa, RNA from Escherichia coli BW25113)
- (1s,2r,3s,4r,5s,6s)-2,3,4,5,6-pentahydroxycyclohexyl 2-amino-4-{[carboxy(imino)methyl]amino}-2,3,4,6-tetradeoxy-alpha-d-arabino-hexopyranoside (379 Da, Ligand)
- Potassium ion (39 Da, Ligand)
- Small ribosomal subunit protein bs18 (9 kDa, Protein from Escherichia coli BW25113)
- 70s ribosomes with antibiotic cocktail (Complex from Escherichia coli BW25113)
- Small ribosomal subunit protein us17 (9 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us11 (13 kDa, Protein from Escherichia coli BW25113)
- Capreomycin ia (686 Da, Protein from Streptomyces californicus)
- Small ribosomal subunit protein us8 (14 kDa, Protein from Escherichia coli BW25113)
- 16s rrna (499 kDa, RNA from Escherichia coli BW25113)
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Small ribosomal subunit protein bs6, non-modified isoform (15 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein bs21 (8 kDa, Protein from Escherichia coli BW25113)
- Small ribosomal subunit protein us4 (23 kDa, Protein from Escherichia coli BW25113)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli BW25113)