KimA from B. subtilis with nucleotide second-messenger c-di-AMP bound
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 8b70
Q-score: 0.536
Fuss MF, Wieferig JP, Corey RA, Hellmich Y, Tascon I, Sousa JS, Stansfeld PJ, Vonck J, Hanelt I
Nat Commun (2023) 14 pp. 3683-3683 [ Pubmed: 37344476 DOI: doi:10.1038/s41467-023-38944-1 ]
- Kima with bound c-di-amp (134 kDa, Complex from Bacillus subtilis)
- Potassium transporter kima (66 kDa, Protein from Bacillus subtilis)
- Potassium ion (39 Da, Ligand)
- (2r,3r,3as,5r,7ar,9r,10r,10as,12r,14ar)-2,9-bis(6-amino-9h-purin-9-yl)octahydro-2h,7h-difuro[3,2-d:3',2'-j][1,3,7,9,2,8 ]tetraoxadiphosphacyclododecine-3,5,10,12-tetrol 5,12-dioxide (658 Da, Ligand)
- Dodecyl-beta-d-maltoside (510 Da, Ligand)
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7zrd
Q-score: 0.504
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase kdpf subunit (3 kDa, Protein from Escherichia coli)
- Vanadate ion (114 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
Cryo-EM map of the WT KdpFABC complex in the E1-P tight conformation, under turnover conditions
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7zre
Q-score: 0.487
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
Cryo-EM map of the WT KdpFABC complex in the E1_ATPearly conformation, under turnover conditions
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7zrg
Q-score: 0.465
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
Cryo-EM structure of the KdpFABC complex in a nucleotide-free E1 conformation loaded with K+
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7zrh
Q-score: 0.485
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
Cryo-EM structure of the KdpFABC complex in a nucleotide-free E1 conformation loaded with K+
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7zri
Q-score: 0.457
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
Cryo-EM structure of the KdpFABC complex in a nucleotide-free E1 conformation loaded with K+
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7zrj
Q-score: 0.429
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
Cryo-EM map of the WT KdpFABC complex in the E1-P_ADP conformation, under turnover conditions
Resolution: 3.1 Å
EM Method: Single-particle
Fitted PDBs: 7zrk
Q-score: 0.529
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Cardiolipin (1 kDa, Ligand)
- Potassium ion (39 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7zrl
Q-score: 0.42
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli K-12)
- Kdpfabc (157 kDa, Complex from Escherichia coli K-12)
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli K-12)
- Potassium ion (39 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli K-12)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli K-12)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7zrm
Q-score: 0.446
Silberberg JM, Stock C, Hielkema L, Corey RA, Rheinberger J, Wunnicke D, Dubach VRA, Stansfeld PJ, Hanelt I, Paulino C
eLife (2022) 11 [ Pubmed: 36255052 DOI: doi:10.7554/eLife.80988 ]
- Potassium-transporting atpase kdpf subunit (2 kDa, Protein from Escherichia coli)
- Potassium-transporting atpase atp-binding subunit (72 kDa, Protein from Escherichia coli)
- Kdpfabc (157 kDa, Complex from Escherichia coli)
- Potassium-transporting atpase kdpc subunit (20 kDa, Protein from Escherichia coli)
- Potassium ion (39 Da, Ligand)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- Potassium-transporting atpase potassium-binding subunit (59 kDa, Protein from Escherichia coli)