Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8q7h
Q-score: 0.39
Horn-Ghetko D, Hopf LVM, Tripathi-Giesgen I, Du J, Kostrhon S, Vu DT, Beier V, Steigenberger B, Prabu JR, Stier L, Bruss EM, Mann M, Xiong Y, Schulman BA
Nat Struct Mol Biol (2024) [ DOI: doi:10.1038/s41594-024-01257-y Pubmed: 38605244 ]
- Cullin-9 (281 kDa, Protein from Homo sapiens)
- Unknown (12 kDa, Protein from Homo sapiens)
- Nedd8 (9 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Structure of cul9-rbx1 ubiquitin e3 ligase complex in unneddylated and neddylated conformation - focused cullin dimer (Complex from Homo sapiens)
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
Liwocha J, Li J, Purser N, Rattanasopa C, Maiwald S, Krist DT, Scott DC, Steigenberger B, Prabu JR, Schulman BA, Kleiger G
Nat Struct Mol Biol (2024) 31 pp. 378-389 [ DOI: doi:10.1038/s41594-023-01206-1 Pubmed: 38326650 ]
- Elongin-c (12 kDa, Protein from Homo sapiens)
- Elongin-b (13 kDa, Protein from Homo sapiens)
- Elongin-b (elob), elongin-c (eloc), protein fem-1 homolog c (fem1c) and trapped ube2r2-donor ub-acceptor ub-sil1 peptide (Complex from Homo sapiens)
- Ring e3 ligase (rbx1) and cullin-2 (cul2) (Complex from Homo sapiens)
- K48-linked ubiquitin chain formation with a cullin-ring e3 ligase and cdc34:nedd8-cul2-rbx1-elob/c-fem1c with trapped ube2r2-donor ub-acceptor ub-sil1 peptide (190 kDa, Complex)
- Protein fem-1 homolog c (68 kDa, Protein from Homo sapiens)
- Polyubiquitin-c,nucleotide exchange factor sil1 (79 kDa, Protein from Homo sapiens)
- Cullin-2 (87 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
Liwocha J, Li J, Purser N, Rattanasopa C, Maiwald S, Krist DT, Scott DC, Steigenberger B, Prabu JR, Schulman BA, Kleiger G
Nat Struct Mol Biol (2024) 31 pp. 378-389 [ DOI: doi:10.1038/s41594-023-01206-1 Pubmed: 38326650 ]
- Cdc34, nedd8, fem1c with ube2r2-donor ub-acceptor ub-sil1 peptide and k48-linked ubiquitin chain (Complex from Homo sapiens)
- Ubiquitin (77 kDa, Protein from Homo sapiens)
- K48-linked ubiquitin chain formation with a cullin-ring e3 ligase and cdc34:nedd8-cul2-rbx1-elob/c-fem1c with trapped ube2r2-donor ub-acceptor ub-sil1 peptide (190 kDa, Complex)
- Protein fem-1 homolog c (68 kDa, Protein from Homo sapiens)
- Polyubiquitin-c,nucleotide exchange factor sil1 (79 kDa, Protein from Homo sapiens)
- Ring e3 ligase (rbx1) (Complex from Homo sapiens)
- Ubiquitin-conjugating enzyme e2 r2 (27 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
Liwocha J, Li J, Purser N, Rattanasopa C, Maiwald S, Krist DT, Scott DC, Steigenberger B, Prabu JR, Schulman BA, Kleiger G
Nat Struct Mol Biol (2024) 31 pp. 378-389 [ DOI: doi:10.1038/s41594-023-01206-1 Pubmed: 38326650 ]
- Ubiquitin (77 kDa, Protein from Homo sapiens)
- K48-linked ubiquitin chain formation with a cullin-ring e3 ligase and cdc34:nedd8-cul2-rbx1-elob/c-fem1c with trapped ube2r2-donor ub-acceptor ub-sil1 peptide (190 kDa, Complex)
- Protein fem-1 homolog c (68 kDa, Protein from Homo sapiens)
- Polyubiquitin-c,nucleotide exchange factor sil1 (79 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
- Ring e3 ligase (rbx1) (Complex from Homo sapiens)
- Cdc34, nedd8, fem1c with ube2r2-donor ub-acceptor ub-sil1 peptide and k48-linked ubiquitin chain (Complex from 9606)
- Ubiquitin-conjugating enzyme e2 r2 (27 kDa, Protein from Homo sapiens)
Liwocha J, Li J, Purser N, Rattanasopa C, Maiwald S, Krist DT, Scott DC, Steigenberger B, Prabu JR, Schulman BA, Kleiger G
Nat Struct Mol Biol (2024) 31 pp. 378-389 [ DOI: doi:10.1038/s41594-023-01206-1 Pubmed: 38326650 ]
- Cdc34, nedd8, fem1c with ube2r2-donor ub-acceptor ub-sil1 peptide and k48-linked ubiquitin chain (Complex from Homo sapiens)
- Ubiquitin (77 kDa, Protein from Homo sapiens)
- Ring e3 ligase (rbx1) and cullin-2 (cul2) (Complex from Homo sapiens)
- K48-linked ubiquitin chain formation with a cullin-ring e3 ligase and cdc34:nedd8-cul2-rbx1-elob/c-fem1c with trapped ube2r2-donor ub-acceptor ub-sil1 peptide (190 kDa, Complex)
- Protein fem-1 homolog c (68 kDa, Protein from Homo sapiens)
- Polyubiquitin-c,nucleotide exchange factor sil1 (79 kDa, Protein from Homo sapiens)
- Cullin-2 (87 kDa, Protein from Homo sapiens)
- Ubiquitin-conjugating enzyme e2 r2 (27 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
Liwocha J, Li J, Purser N, Rattanasopa C, Maiwald S, Krist DT, Scott DC, Steigenberger B, Prabu JR, Schulman BA, Kleiger G
Nat Struct Mol Biol (2024) 31 pp. 378-389 [ DOI: doi:10.1038/s41594-023-01206-1 Pubmed: 38326650 ]
- Cdc34, nedd8, fem1c with ube2r2-donor ub-acceptor ub-sil1 peptide and k48-linked ubiquitin chain (Complex from Homo sapiens)
- Ubiquitin (77 kDa, Protein from Homo sapiens)
- Ring e3 ligase (rbx1) and cullin-2 (cul2) (Complex from Homo sapiens)
- K48-linked ubiquitin chain formation with a cullin-ring e3 ligase and cdc34:nedd8-cul2-rbx1-elob/c-fem1c with trapped ube2r2-donor ub-acceptor ub-sil1 peptide (190 kDa, Complex)
- Protein fem-1 homolog c (68 kDa, Protein from Homo sapiens)
- Polyubiquitin-c,nucleotide exchange factor sil1 (79 kDa, Protein from Homo sapiens)
- Cullin-2 (87 kDa, Protein from Homo sapiens)
- Ubiquitin-conjugating enzyme e2 r2 (27 kDa, Protein from Homo sapiens)
- E3 ubiquitin-protein ligase rbx1 (12 kDa, Protein from Homo sapiens)
cryo-EM structure of neddylated CUL2-RBX1-ELOB-ELOC-FEM1B bound with the C-degron of CDK5R1
Resolution: 3.54 Å
EM Method: Single-particle
Fitted PDBs: 8wqc
Q-score: 0.352
Chen X, Raiff A, Li S, Guo Q, Zhang J, Zhou H, Timms RT, Yao X, Elledge SJ, Koren I, Zhang K, Xu C
Nat Commun (2024) 15 pp. 3558-3558 [ DOI: doi:10.1038/s41467-024-47890-5 Pubmed: 38670995 ]
- E3 ubiquitin-protein ligase rbx1, n-terminally processed (11 kDa, Protein from Homo sapiens)
- Cryo-em structure of neddylated cul2-rbx1-elob-eloc-fem1b bound with the c-degron of cdk5r1 (Complex from Homo sapiens)
- Elongin-b (13 kDa, Protein from Homo sapiens)
- Cullin-2 (87 kDa, Protein from Homo sapiens)
- Elongin-c (10 kDa, Protein from Homo sapiens)
- Nedd8 (8 kDa, Protein from Homo sapiens)
- Protein fem-1 homolog b (70 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
Resolution: 4.09 Å
EM Method: Single-particle
Fitted PDBs: 8wqg
Q-score: 0.265
Chen X, Raiff A, Li S, Guo Q, Zhang J, Zhou H, Timms RT, Yao X, Elledge SJ, Koren I, Zhang K, Xu C
Nat Commun (2024) 15 pp. 3558-3558 [ DOI: doi:10.1038/s41467-024-47890-5 Pubmed: 38670995 ]
- E3 ubiquitin-protein ligase rbx1, n-terminally processed (11 kDa, Protein from Homo sapiens)
- Elongin-b (13 kDa, Protein from Homo sapiens)
- Cullin-2 (87 kDa, Protein from Homo sapiens)
- Elongin-c (10 kDa, Protein from Homo sapiens)
- Nedd8 (8 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Protein fem-1 homolog b (70 kDa, Protein from Homo sapiens)
- Cryo-em structure of neddylated cul2-rbx1-elob-eloc-fem1b bound with the c-degron of ccdc89 (conformation 1) (Complex from Homo sapiens)
Crowe C, Nakasone MA, Chandler S, Tatham MH, Makukhin N, Hay RT, Ciulli A
bioRxiv (2024) [ DOI: doi:10.1101/2024.02.05.578957 ]
Li J, Purser N, Liwocha J, Scott DC, Byers HA, Steigenberger B, Hill S, Tripathi-Giesgen I, Hinkle T, Hansen FM, Prabu JR, Radhakrishnan SK, Kirkpatrick DS, Reichermeier KM, Schulman BA, Kleiger G
Mol Cell (2024) 84 pp. 1304-1320.e16 [ DOI: doi:10.1016/j.molcel.2024.01.022 Pubmed: 38382526 ]