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KIF14[391-772] dimer two-heads-bound state - AMP-PNP in complex with a microtubule
Resolution: 3.8 Å
EM Method: Helical reconstruction
Fitted PDBs: 6wwh
Q-score: 0.353
Benoit MPMH, Asenjo AB, Paydar M, Dhakal S, Kwok BH, Sosa H
Nat Commun (2021) 12 pp. 3637-3637 [ Pubmed: 34131133 DOI: doi:10.1038/s41467-021-23581-3 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Kif14[391-772] - amp-pnp (Complex from Mus musculus)
- Kif14[391-772] - amp-pnp in complex with a microtubule (Complex)
- Kinesin-like protein kif14 (43 kDa, Protein from Mus musculus)
- Tubulin beta-2b chain (49 kDa, Protein from Sus scrofa)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Phosphoaminophosphonic acid-adenylate ester (506 Da, Ligand)
- Taxol (853 Da, Ligand)
- Tubulin alpha-1b chain (50 kDa, Protein from Sus scrofa)
- Magnesium ion (24 Da, Ligand)
- Microtubule (Cellular component from Sus scrofa)
Resolution: 2.5 Å
EM Method: Single-particle
Fitted PDBs: 7dco
Q-score: 0.408
Bai R, Wan R, Yan C, Jia Q, Lei J, Shi Y
Science (2021) 371 [ DOI: doi:10.1126/science.abe8863 Pubmed: 33243853 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna (44 kDa, RNA from Saccharomyces cerevisiae)
- Calcium ion (40 Da, Ligand)
- Snu114 isoform 1 (114 kDa, Protein from Saccharomyces cerevisiae)
- Smd1 isoform 1 (16 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae)
- Bj4_g0056610.mrna.1.cds.1 (12 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Saccharomyces cerevisiae)
- Zinc ion (65 Da, Ligand)
- Bj4_g0014900.mrna.1.cds.1 (22 kDa, Protein from Saccharomyces cerevisiae)
- Inositol hexakisphosphate (660 Da, Ligand)
- Bud31 isoform 1 (18 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Saccharomyces cerevisiae)
- Hsh155 isoform 1 (110 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna leakage protein 1 (23 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Sx2_g0027210.mrna.1.cds.1 (14 kDa, Protein from Saccharomyces cerevisiae)
- The activated spliceosome bact complex (Complex from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (8 kDa, Protein from Saccharomyces cerevisiae)
- Cwc27 isoform 1 (35 kDa, Protein from Saccharomyces cerevisiae)
- Magnesium ion (24 Da, Ligand)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Saccharomyces cerevisiae)
- Hlj1_g0043010.mrna.1.cds.1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Clf1 isoform 1 (82 kDa, Protein from Saccharomyces cerevisiae)
- Snt309 isoform 1 (20 kDa, Protein from Saccharomyces cerevisiae)
- Bj4_g0037700.mrna.1.cds.1 (12 kDa, Protein from Saccharomyces cerevisiae)
- Cdc40 isoform 1 (52 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Saccharomyces cerevisiae)
- U6 snrna (35 kDa, RNA from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor atp-dependent rna helicase-like protein prp2 (99 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc2 (29 kDa, Protein from Saccharomyces cerevisiae)
- Cwc22 isoform 1 (67 kDa, Protein from Saccharomyces cerevisiae)
- Sm protein f (9 kDa, Protein from Saccharomyces cerevisiae)
- Hlj1_g0053790.mrna.1.cds.1 (27 kDa, Protein from Saccharomyces cerevisiae)
- Rds3 complex subunit 10 (9 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Prp11 isoform 1 (29 kDa, Protein from Saccharomyces cerevisiae)
- Hsh49 isoform 1 (24 kDa, Protein from Saccharomyces cerevisiae)
- Prp9 isoform 1 (63 kDa, Protein from Saccharomyces cerevisiae)
- Bj4_g0027490.mrna.1.cds.1 (12 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor prp21 (33 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- Water (18 Da, Ligand)
- Rse1 isoform 1 (153 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor spp2 (20 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae)
- Prp8 isoform 1 (279 kDa, Protein from Saccharomyces cerevisiae)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc26 (14 kDa, Protein from Saccharomyces cerevisiae)
- Hlj1_g0054350.mrna.1.cds.1 (37 kDa, Protein from Saccharomyces cerevisiae)
- Syf1 isoform 1 (100 kDa, Protein from Saccharomyces cerevisiae)
- Brr2 isoform 1 (246 kDa, Protein from Saccharomyces cerevisiae)
- Pre-mrna-splicing factor cwc24 (29 kDa, Protein from Saccharomyces cerevisiae)
Outer Dynein Arm-Shulin complex - overall structure (Tetrahymena thermophila)
Resolution: 8.78 Å
EM Method: Single-particle
Fitted PDBs: 6zyw
Q-score: 0.103
Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP
Science (2021) 371 pp. 910-916 [ DOI: doi:10.1126/science.abe0526 Pubmed: 33632841 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila SB210)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Dynein light chain roadblock-type 2 protein (11 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila SB210)
- Tetrahymena thermophila oda dyh4 motor (1 MDa, Complex)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila SB210)
- Shulin (Complex from Tetrahymena thermophila (strain SB210))
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain roadblock-type 2 protein (14 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila SB210)
- Thioredoxin (12 kDa, Protein from Tetrahymena thermophila SB210)
- Shulin (139 kDa, Protein from Tetrahymena thermophila SB210)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila SB210)
- Adenosine-5'-diphosphate (427 Da, Ligand)
- Tetrahymena thermophila oda dyh4 motor (Complex from Tetrahymena thermophila (strain SB210))
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila SB210)
- Outer arm dynein beta heavy chain (530 kDa, Protein from Tetrahymena thermophila SB210)
- Dynein-1-alpha heavy chain, flagellar inner arm i1 complex protein, putative (475 kDa, Protein from Tetrahymena thermophila SB210)
Outer Dynein Arm-Shulin complex - Shulin region from Tetrahymena thermophila
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6zyx
Q-score: 0.398
Mali GR, Ali FA, Lau CK, Begum F, Boulanger J, Howe JD, Chen ZA, Rappsilber J, Skehel M, Carter AP
Science (2021) 371 pp. 910-916 [ DOI: doi:10.1126/science.abe0526 Pubmed: 33632841 ]
- Shulin region from outer dynein arm-shulin complex (tetrahymena thermophila). contains shulin (excluding c3 finger), lc8e, lc8f, lc8d, lc8, lc2a, lc9, dic n-termini and portion of dyh3 tail. (Complex)
- Dynein light chain (13 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dynein (Complex from Tetrahymena thermophila CU428)
- Shulin (Complex from Tetrahymena thermophila CU428)
- Dynein heavy chain, outer arm protein (534 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (12 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain 2a (15 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain (10 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein light chain tctex-type 1 protein (13 kDa, Protein from Tetrahymena thermophila CU428)
- Flagellar outer dynein arm intermediate protein, putative (77 kDa, Protein from Tetrahymena thermophila CU428)
- Shulin (139 kDa, Protein from Tetrahymena thermophila CU428)
- Dynein intermediate chain 2 (77 kDa, Protein from Tetrahymena thermophila CU428)
Outer dynein arm bound to doublet microtubules from C. reinhardtii
Resolution: 7.5 Å
EM Method: Helical reconstruction
Fitted PDBs: 7kzm
Q-score: 0.315
Nat Commun (2021) 12 pp. 477-477 [ DOI: doi:10.1038/s41467-020-20735-7 Pubmed: 33473120 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dynein light chain roadblock lc7a (11 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein 8 kda light chain, flagellar outer arm (10 kDa, Protein from Chlamydomonas reinhardtii)
- Dc1 (13 kDa, Protein from Chlamydomonas reinhardtii)
- Oda complex (Complex from Chlamydomonas reinhardtii)
- Dynein light chain 9 (12 kDa, Protein from Chlamydomonas reinhardtii)
- Outer dynein arm protein 1 (62 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar outer dynein arm heavy chain beta (520 kDa, Protein from Chlamydomonas reinhardtii)
- Heavy chain alpha (504 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein, 78 kda intermediate chain, flagellar outer arm (76 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin beta (49 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar outer dynein arm light chain 2 (15 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein 18 kda light chain, flagellar outer arm (17 kDa, Protein from Chlamydomonas reinhardtii)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Dynein 11 kda light chain, flagellar outer arm (13 kDa, Protein from Chlamydomonas reinhardtii)
- Outer dynein arm-docking complex subunit 1 (83 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain roadblock lc7b (11 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin alpha (49 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein, 70 kda intermediate chain, flagellar outer arm (63 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein light chain 10 (12 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein gamma chain, flagellar outer arm (513 kDa, Protein from Chlamydomonas reinhardtii)
- Outer dynein arm-docking complex protein dc3 (21 kDa, Protein from Chlamydomonas reinhardtii)
- Dc2 (14 kDa, Protein from Chlamydomonas reinhardtii)
- Magnesium ion (24 Da, Ligand)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 7ju4
Q-score: 0.431
Gui M, Ma M, Sze-Tu E, Wang X, Koh F, Zhong ED, Berger B, Davis JH, Dutcher SK, Zhang R, Brown A
Nat Struct Mol Biol (2021) 28 pp. 29-37 [ DOI: doi:10.1038/s41594-020-00530-0 Pubmed: 33318703 ]
- Dynein regulatory complex subunit 4 (55 kDa, Protein from Chlamydomonas reinhardtii)
- Cfap91 domain-containing protein (134 kDa, Protein from Chlamydomonas reinhardtii)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Dynein 8 kda light chain, flagellar outer arm (10 kDa, Protein from Chlamydomonas reinhardtii)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- Complex of radial spoke 2 stalk, idac, and n-drc attached with doublet microtubule (Complex from Chlamydomonas reinhardtii)
- Radial spoke protein 3 (56 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein regulatory complex protein 1 (79 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin beta (49 kDa, Protein from Chlamydomonas reinhardtii)
- Unknown protein (6 kDa, Protein from Chlamydomonas reinhardtii)
- Radial spoke protein 11 (21 kDa, Protein from Chlamydomonas reinhardtii)
- Actin (41 kDa, Protein from Chlamydomonas reinhardtii)
- Fap253 (75 kDa, Protein from Chlamydomonas reinhardtii)
- Dynein regulatory complex subunit 2 (64 kDa, Protein from Chlamydomonas reinhardtii)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 28 kda inner dynein arm light chain, axonemal (28 kDa, Protein from Chlamydomonas reinhardtii)
- Radial spoke protein 15 (35 kDa, Protein from Chlamydomonas reinhardtii)
- Tubulin alpha (49 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar-associated protein 172 (101 kDa, Protein from Chlamydomonas reinhardtii)
- Fap207 (28 kDa, Protein from Chlamydomonas reinhardtii)
- Flagellar-associated protein 59 (105 kDa, Protein from Chlamydomonas reinhardtii)
- Radial spoke protein 7 (54 kDa, Protein from Chlamydomonas reinhardtii)
- Radial spike protein 8 (40 kDa, Protein from Chlamydomonas reinhardtii)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state B1
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 6rxu
Q-score: 0.44
Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E
Mol Cell (2019) 75 pp. 1256-1269.e7 [ DOI: doi:10.1016/j.molcel.2019.06.032 Pubmed: 31378463 ]
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Utp3 (73 kDa, Protein from Chaetomium thermophilum)
- Utp4 (97 kDa, Protein from Chaetomium thermophilum)
- Utp6 (46 kDa, Protein from Chaetomium thermophilum)
- Putative ribosomal protein (17 kDa, Protein from Chaetomium thermophilum)
- Krr1 small subunit processome component (37 kDa, Protein from Chaetomium thermophilum)
- Putative u3 small nucleolar ribonucleoprotein (34 kDa, Protein from Chaetomium thermophilum)
- Snu13 (13 kDa, Protein from Chaetomium thermophilum)
- Faf1 (34 kDa, Protein from Chaetomium thermophilum)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum)
- Utp8 (102 kDa, Protein from Chaetomium thermophilum)
- Utp10 (198 kDa, Protein from Chaetomium thermophilum)
- U3 snorna (73 kDa, RNA from Chaetomium thermophilum)
- Utp30 (43 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s4 (29 kDa, Protein from Chaetomium thermophilum)
- Nop58 (64 kDa, Protein from Chaetomium thermophilum)
- Utp7 (63 kDa, Protein from Chaetomium thermophilum)
- Utp18 (69 kDa, Protein from Chaetomium thermophilum)
- Putative u3 small nucleolar ribonucleoprotein protein (86 kDa, Protein from Chaetomium thermophilum)
- Ribosome biogenesis protein enp2 (75 kDa, Protein from Chaetomium thermophilum)
- 90s pre-ribosome, state b1 (Complex from Chaetomium thermophilum)
- Imp3 (21 kDa, Protein from Chaetomium thermophilum)
- Bms1 (134 kDa, Protein from Chaetomium thermophilum)
- Putative u3 snornp protein (115 kDa, Protein from Chaetomium thermophilum)
- Pre-rrna-processing protein pno1 (28 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s7 (23 kDa, Protein from Chaetomium thermophilum)
- Utp16 (40 kDa, Protein from Chaetomium thermophilum)
- 35s rrna (758 kDa, RNA from Chaetomium thermophilum)
- Noc4 (63 kDa, Protein from Chaetomium thermophilum)
- Utp17 (105 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s24 (15 kDa, Protein from Chaetomium thermophilum)
- Magnesium ion (24 Da, Ligand)
- Bfr2 (69 kDa, Protein from Chaetomium thermophilum)
- Enp1 (54 kDa, Protein from Chaetomium thermophilum)
- Kre33 (119 kDa, Protein from Chaetomium thermophilum)
- Emg1 (27 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s8 (23 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum)
- Utp14 (104 kDa, Protein from Chaetomium thermophilum)
- Utp5 (44 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar rna-associated protein 11 (31 kDa, Protein from Chaetomium thermophilum)
- Periodic tryptophan protein 2-like protein (100 kDa, Protein from Chaetomium thermophilum)
- U3 small nucleolar ribonucleoprotein protein lcp5-like protein (42 kDa, Protein from Chaetomium thermophilum)
- Sof1 (50 kDa, Protein from Chaetomium thermophilum)
- Rrp9 (69 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum)
- Utp24 (22 kDa, Protein from Chaetomium thermophilum)
- Nop1 (32 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s6 (27 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum)
- Fcf2 (22 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s11-like protein (18 kDa, Protein from Chaetomium thermophilum)
- Utp15 (60 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s1 (29 kDa, Protein from Chaetomium thermophilum)
- Nop56 (57 kDa, Protein from Chaetomium thermophilum)
- Utp12 (107 kDa, Protein from Chaetomium thermophilum)
- Utp2 (103 kDa, Protein from Chaetomium thermophilum)
- Utp20 (295 kDa, Protein from Chaetomium thermophilum)
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum)
- Rna 3'-terminal phosphate cyclase-like protein (43 kDa, Protein from Chaetomium thermophilum)
- Utp13 (100 kDa, Protein from Chaetomium thermophilum)
Cryo-EM structure of the 90S pre-ribosome (Kre33-Noc4) from Chaetomium thermophilum, state B2
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 6rxv
Q-score: 0.371
Cheng J, Bassler J, Fischer P, Lau B, Kellner N, Kunze R, Griesel S, Kallas M, Berninghausen O, Strauss D, Beckmann R, Hurt E
Mol Cell (2019) 75 pp. 1256-1269.e7 [ DOI: doi:10.1016/j.molcel.2019.06.032 Pubmed: 31378463 ]
- U3 snorna (73 kDa, RNA from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nop58 (64 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp17 (105 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Sof1 (50 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Noc4 (63 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Snu13 (13 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s14-like protein (16 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Bms1 (134 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Zinc ion (65 Da, Ligand)
- Utp24 (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Krr1 small subunit processome component (37 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp13 (100 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nop1 (32 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Bfr2 (69 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Faf1 (34 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s1 (29 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s13-like protein (16 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp8 (102 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp10 (198 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s22-like protein (14 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s23-like protein (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp4 (97 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Pre-rrna-processing protein pno1 (28 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s24 (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Nop56 (57 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp18 (69 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rps18 (17 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 small nucleolar rna-associated protein 22 (131 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp21 (115 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 35s ribosomal rna (758 kDa, RNA from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s4 (29 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 small nucleolar rna-associated protein 11 (31 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp2 (103 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp16 (40 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 90s pre-ribosome, state b2 (Complex from Chaetomium thermophilum)
- Magnesium ion (24 Da, Ligand)
- 40s ribosomal protein s28-like protein (7 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s8 (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s9-like protein (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rrp9 (69 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s11-like protein (18 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s7 (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Ribosome biogenesis protein enp2 (75 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Imp3 (21 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Emg1 (27 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s16-like protein (15 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s6 (27 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Enp1 (54 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp7 (63 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp15 (60 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- 40s ribosomal protein s5-like protein (23 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Imp4 (34 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp14 (104 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp6 (46 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Kre33 (119 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Fcf2 (22 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rrp7 (36 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp20 (295 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp3 (73 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- U3 small nucleolar ribonucleoprotein protein lcp5-like protein (42 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Periodic tryptophan protein 2-like protein (100 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp12 (107 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Mpp10 (86 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Rna 3'-terminal phosphate cyclase-like protein (43 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp5 (44 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
- Utp30 (43 kDa, Protein from Chaetomium thermophilum (strain DSM 1495 / CBS 144.50 / IMI 039719))
Cryo-EM structure of the yeast B*-a1 complex at an average resolution of 3.6 angstrom
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 6j6h
Q-score: 0.283
Wan R, Bai R, Yan C, Lei J, Shi Y
Cell (2019) 177 pp. 339-351.e13 [ DOI: doi:10.1016/j.cell.2019.02.006 Pubmed: 30879786 ]
- U6 snrna (35 kDa, RNA from Baker's yeast)
- Pre-mrna-processing factor 17 (52 kDa, Protein from Baker's yeast)
- Zinc ion (65 Da, Ligand)
- Pre-mrna-splicing factor prp46 (50 kDa, Protein from Baker's yeast)
- Inositol hexakisphosphate (660 Da, Ligand)
- Pre-mrna-processing factor 19 (56 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor snu114 (114 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d3 (11 kDa, Protein from Baker's yeast)
- Act1 pre-mrna (217 kDa, RNA from Saccharomyces cerevisiae S288c)
- Pre-mrna-splicing factor cef1 (67 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor bud31 (18 kDa, Protein from Baker's yeast)
- Catalytically activated spliceosome b*-a1 complex (Complex from Saccharomyces cerevisiae S288C)
- Pre-mrna-splicing factor snt309 (20 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc21 (15 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf2 (24 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Baker's yeast)
- U5 snrna (68 kDa, RNA from Saccharomyces cerevisiae S288c)
- Small nuclear ribonucleoprotein sm d2 (12 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein-associated protein b (22 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc2 (38 kDa, Protein from Baker's yeast)
- U2 small nuclear ribonucleoprotein a' (27 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor slt11 (40 kDa, Protein from Baker's yeast)
- Pre-mrna-processing protein 45 (42 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor syf1 (100 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein sm d1 (16 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor isy1 (28 kDa, Protein from Baker's yeast)
- U2 snrna (376 kDa, RNA from Saccharomyces cerevisiae S288c)
- U2 small nuclear ribonucleoprotein b'' (12 kDa, Protein from Baker's yeast)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Pre-mrna-splicing factor clf1 (82 kDa, Protein from Baker's yeast)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor 8 (279 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc15 (19 kDa, Protein from Baker's yeast)
- Pre-mrna-splicing factor cwc22 (67 kDa, Protein from Baker's yeast)
- Magnesium ion (24 Da, Ligand)
Cryo-EM structure of a human activated spliceosome (early Bact) at 4.9 angstrom.
Resolution: 4.9 Å
EM Method: Single-particle
Fitted PDBs: 5z58
Q-score: 0.131
Zhang X, Yan C, Zhan X, Li L, Lei J, Shi Y
Cell Res (2018) 28 pp. 307-322 [ DOI: doi:10.1038/cr.2018.14 Pubmed: 29360106 ]
- Pre-mrna (87 kDa, RNA from unidentified adenovirus)
- Early bact spliceosome (Complex from Homo sapiens)
- U2 small nuclear ribonucleoprotein a' (28 kDa, Protein from Human)
- Small nuclear ribonucleoprotein-associated proteins b and b' (23 kDa, Protein from Human)
- Splicing factor 3a subunit 2 (49 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Phd finger-like domain-containing protein 5a (12 kDa, Protein from Human)
- Pre-mrna-processing-splicing factor 8 (273 kDa, Protein from Human)
- Small nuclear ribonucleoprotein sm d1 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 6 (14 kDa, Protein from Human)
- Inositol hexakisphosphate (660 Da, Ligand)
- Splicing factor 3b subunit 4 (44 kDa, Protein from Human)
- U5 snrna (37 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d2 (13 kDa, Protein from Human)
- Small nuclear ribonucleoprotein g (8 kDa, Protein from Human)
- 116 kda u5 small nuclear ribonucleoprotein component (109 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 200 kda helicase (244 kDa, Protein from Human)
- Peptidyl-prolyl cis-trans isomerase cwc27 homolog (53 kDa, Protein from Human)
- U2 small nuclear ribonucleoprotein b'' (25 kDa, Protein from Human)
- Bud13 homolog (70 kDa, Protein from Human)
- Pre-mrna-splicing factor cwc22 homolog (105 kDa, Protein from Human)
- U6 snrna (34 kDa, RNA from Human)
- Small nuclear ribonucleoprotein sm d3 (13 kDa, Protein from Human)
- Splicing factor 3b subunit 1 (146 kDa, Protein from Human)
- Splicing factor 3b subunit 3 (135 kDa, Protein from Human)
- Smad nuclear-interacting protein 1 (45 kDa, Protein from Human)
- Small nuclear ribonucleoprotein f (9 kDa, Protein from Human)
- Small nuclear ribonucleoprotein e (10 kDa, Protein from Human)
- Splicing factor 3a subunit 3 (58 kDa, Protein from Human)
- Pleiotropic regulator 1 (57 kDa, Protein from Human)
- Splicing factor 3a subunit 1 (88 kDa, Protein from Human)
- U2 snrna (60 kDa, RNA from Human)
- Splicing factor 3b subunit 5 (10 kDa, Protein from Human)
- Rna-binding motif protein, x-linked 2 (37 kDa, Protein from Human)
- U5 small nuclear ribonucleoprotein 40 kda protein (39 kDa, Protein from Human)
- Cell division cycle 5-like protein (92 kDa, Protein from Human)
- Skip (62 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Spliceosome-associated protein cwc15 homolog (26 kDa, Protein from Human)
- Ring finger protein 113a (38 kDa, Protein from Human)
- Pre-mrna-splicing factor atp-dependent rna helicase dhx16 (119 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Splicing factor 3b subunit 2 (100 kDa, Protein from Human)
- Crooked neck-like protein 1 (100 kDa, Protein from Human)