Human nucleolar pre-60S ribosomal subunit (State H) - Composite map
Resolution: 2.91 Å
EM Method: Single-particle
Fitted PDBs: 8fl0
Q-score: 0.49
Science (2023) 381 pp. eadh3892-eadh3892 [ Pubmed: 37410842 DOI: doi:10.1126/science.adh3892 ]
- 60s ribosomal protein l11 (20 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l32 (15 kDa, Protein from Homo sapiens)
- Nucleolar gtp-binding protein 2 (83 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l4 (47 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l13a (23 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- Coiled-coil domain-containing protein 86 (40 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l21 (18 kDa, Protein from Homo sapiens)
- 5s rrna (38 kDa, RNA from Homo sapiens)
- 60s ribosomal protein l18a (20 kDa, Protein from Homo sapiens)
- Protein llp homolog (15 kDa, Protein from Homo sapiens)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- 60s ribosomal protein l23 (14 kDa, Protein from Homo sapiens)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l14 (23 kDa, Protein from Homo sapiens)
- Notchless protein homolog 1 (53 kDa, Protein from Homo sapiens)
- Human nucleolar pre-60s ribosomal subunit (state h) (Complex from Homo sapiens)
- Probable ribosome biogenesis protein rlp24 (19 kDa, Protein from Homo sapiens)
- Ribosome production factor 2 homolog (35 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l12 (17 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l35a (12 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l7 (29 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l9 (21 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l5 (34 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Guanine nucleotide-binding protein-like 3 (62 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l3 (46 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l28 (15 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l6 (32 kDa, Protein from Homo sapiens)
- Gtp-binding protein 4 (74 kDa, Protein from Homo sapiens)
- Ribosome biogenesis regulatory protein homolog (41 kDa, Protein from Homo sapiens)
- Ribosome biogenesis protein nsa2 homolog (30 kDa, Protein from Homo sapiens)
- 28s rrna (1 MDa, RNA from Homo sapiens)
- Mrna turnover protein 4 homolog (27 kDa, Protein from Homo sapiens)
- 60s ribosomal protein l18 (21 kDa, Protein from Homo sapiens)
- Potassium ion (39 Da, Ligand)
Human nuclear pre-60S ribosomal subunit (State I1) - Composite map
Resolution: 2.67 Å
EM Method: Single-particle
Fitted PDBs: 8fl2
Q-score: 0.544
Science (2023) 381 pp. eadh3892-eadh3892 [ DOI: doi:10.1126/science.adh3892 Pubmed: 37410842 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Gtp-binding protein 4 (74 kDa, Protein from human)
- 60s ribosomal protein l26 (17 kDa, Protein from human)
- 28s rrna (1 MDa, RNA from human)
- Pescadillo homolog (68 kDa, Protein from human)
- 60s ribosomal protein l38 (8 kDa, Protein from human)
- 5s rrna (38 kDa, RNA from human)
- Human nuclear pre-60s ribosomal subunit (state i1) (Complex from Homo sapiens)
- 60s ribosomal protein l15 (24 kDa, Protein from human)
- 60s ribosomal protein l36 (12 kDa, Protein from human)
- Ribosomal l1 domain-containing protein 1 (55 kDa, Protein from human)
- 60s ribosomal protein l27a (16 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l18a (20 kDa, Protein from human)
- Zinc finger protein 593 (15 kDa, Protein from human)
- Protein llp homolog (15 kDa, Protein from human)
- Mki67 fha domain-interacting nucleolar phosphoprotein (34 kDa, Protein from human)
- 60s ribosomal protein l23a (17 kDa, Protein from human)
- 60s ribosomal protein l8 (28 kDa, Protein from human)
- 60s ribosomal protein l7-like 1 (29 kDa, Protein from human)
- 60s ribosomal protein l35a (12 kDa, Protein from human)
- Ribosomal biogenesis protein las1l (83 kDa, Protein from human)
- 60s ribosomal protein l37a (10 kDa, Protein from human)
- 60s ribosomal protein l37 (11 kDa, Protein from human)
- Testis-expressed protein 10 (105 kDa, Protein from human)
- Proline-, glutamic acid- and leucine-rich protein 1 (119 kDa, Protein from human)
- 60s ribosomal protein l30 (12 kDa, Protein from human)
- Potassium ion (39 Da, Ligand)
- 60s ribosomal protein l18 (21 kDa, Protein from human)
- 60s ribosomal protein l13 (24 kDa, Protein from human)
- 60s ribosomal protein l34 (13 kDa, Protein from human)
- Guanine nucleotide-binding protein-like 3 (62 kDa, Protein from human)
- 60s ribosomal protein l35 (14 kDa, Protein from human)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Coiled-coil domain-containing protein 86 (40 kDa, Protein from human)
- 60s ribosomal protein l10a (24 kDa, Protein from human)
- 60s ribosomal protein l23 (14 kDa, Protein from human)
- Nucleolar gtp-binding protein 2 (83 kDa, Protein from human)
- Its2 rrna (376 kDa, RNA from human)
- 60s ribosomal protein l7 (29 kDa, Protein from human)
- 60s ribosomal protein l32 (15 kDa, Protein from human)
- 60s ribosomal protein l22 (14 kDa, Protein from human)
- 60s ribosomal protein l11 (20 kDa, Protein from human)
- 60s ribosomal protein l7a (30 kDa, Protein from human)
- 60s ribosomal protein l9 (21 kDa, Protein from human)
- Wd repeat-containing protein 18 (47 kDa, Protein from human)
- 60s ribosomal protein l21 (18 kDa, Protein from human)
- 60s ribosomal protein l27 (15 kDa, Protein from human)
- 60s ribosomal protein l4 (47 kDa, Protein from human)
- Probable ribosome biogenesis protein rlp24 (19 kDa, Protein from human)
- 60s ribosomal protein l5 (34 kDa, Protein from human)
- 60s ribosomal protein l6 (32 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l13a (23 kDa, Protein from human)
- 60s ribosomal protein l31 (14 kDa, Protein from human)
- 60s ribosomal protein l17 (21 kDa, Protein from human)
- Ribosome biogenesis protein nsa2 homolog (30 kDa, Protein from human)
- Ribosome biogenesis protein nop53 (54 kDa, Protein from human)
- 60s ribosomal protein l28 (15 kDa, Protein from human)
- Notchless protein homolog 1 (53 kDa, Protein from human)
- 60s ribosomal protein l12 (17 kDa, Protein from human)
- Mrna turnover protein 4 homolog (27 kDa, Protein from human)
- 5.8s rrna (50 kDa, RNA from human)
- 60s ribosomal protein l19 (23 kDa, Protein from human)
- 60s ribosomal protein l14 (23 kDa, Protein from human)
- 60s ribosomal protein l3 (46 kDa, Protein from human)
- 60s ribosomal protein l39 (6 kDa, Protein from human)
- Protein sda1 homolog (80 kDa, Protein from human)
Human nuclear pre-60S ribosomal subunit (State I2) - Composite map
Resolution: 2.53 Å
EM Method: Single-particle
Fitted PDBs: 8fl3
Q-score: 0.563
Science (2023) 381 pp. eadh3892-eadh3892 [ DOI: doi:10.1126/science.adh3892 Pubmed: 37410842 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Gtp-binding protein 4 (74 kDa, Protein from human)
- 60s ribosomal protein l26 (17 kDa, Protein from human)
- Human nuclear pre-60s ribosomal subunit (state i2) (Complex from Homo sapiens)
- 28s rrna (1 MDa, RNA from human)
- Pescadillo homolog (68 kDa, Protein from human)
- 60s ribosomal protein l38 (8 kDa, Protein from human)
- 5s rrna (38 kDa, RNA from human)
- 60s ribosomal protein l36 (12 kDa, Protein from human)
- 60s ribosomal protein l15 (24 kDa, Protein from human)
- 60s ribosomal protein l27a (16 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 60s ribosomal protein l18a (20 kDa, Protein from human)
- Zinc finger protein 593 (15 kDa, Protein from human)
- Protein llp homolog (15 kDa, Protein from human)
- Mki67 fha domain-interacting nucleolar phosphoprotein (34 kDa, Protein from human)
- 60s ribosomal protein l23a (17 kDa, Protein from human)
- 60s ribosomal protein l8 (28 kDa, Protein from human)
- 60s ribosomal protein l7-like 1 (29 kDa, Protein from human)
- 60s ribosomal protein l35a (12 kDa, Protein from human)
- Ribosomal biogenesis protein las1l (83 kDa, Protein from human)
- 60s ribosomal protein l37a (10 kDa, Protein from human)
- 60s ribosomal protein l37 (11 kDa, Protein from human)
- Testis-expressed protein 10 (105 kDa, Protein from human)
- Proline-, glutamic acid- and leucine-rich protein 1 (119 kDa, Protein from human)
- 60s ribosomal protein l30 (12 kDa, Protein from human)
- Potassium ion (39 Da, Ligand)
- 60s ribosomal protein l18 (21 kDa, Protein from human)
- 60s ribosomal protein l13 (24 kDa, Protein from human)
- 60s ribosomal protein l34 (13 kDa, Protein from human)
- Guanine nucleotide-binding protein-like 3 (62 kDa, Protein from human)
- 60s ribosomal protein l35 (14 kDa, Protein from human)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Coiled-coil domain-containing protein 86 (40 kDa, Protein from human)
- 60s ribosomal protein l10a (24 kDa, Protein from human)
- 60s ribosomal protein l23 (14 kDa, Protein from human)
- Nucleolar gtp-binding protein 2 (83 kDa, Protein from human)
- 60s ribosomal protein l7 (29 kDa, Protein from human)
- 60s ribosomal protein l32 (15 kDa, Protein from human)
- 60s ribosomal protein l22 (14 kDa, Protein from human)
- 60s ribosomal protein l11 (20 kDa, Protein from human)
- 60s ribosomal protein l7a (30 kDa, Protein from human)
- 60s ribosomal protein l9 (21 kDa, Protein from human)
- Wd repeat-containing protein 18 (47 kDa, Protein from human)
- 60s ribosomal protein l21 (18 kDa, Protein from human)
- 60s ribosomal protein l27 (15 kDa, Protein from human)
- 60s ribosomal protein l4 (47 kDa, Protein from human)
- Probable ribosome biogenesis protein rlp24 (19 kDa, Protein from human)
- 60s ribosomal protein l5 (34 kDa, Protein from human)
- 60s ribosomal protein l6 (32 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l13a (23 kDa, Protein from human)
- 60s ribosomal protein l31 (14 kDa, Protein from human)
- 60s ribosomal protein l17 (21 kDa, Protein from human)
- Ribosome biogenesis protein nsa2 homolog (30 kDa, Protein from human)
- Ribosome biogenesis protein nop53 (54 kDa, Protein from human)
- 60s ribosomal protein l28 (15 kDa, Protein from human)
- Notchless protein homolog 1 (53 kDa, Protein from human)
- 60s ribosomal protein l12 (17 kDa, Protein from human)
- Mrna turnover protein 4 homolog (27 kDa, Protein from human)
- 5.8s rrna (50 kDa, RNA from human)
- 60s ribosomal protein l19 (23 kDa, Protein from human)
- 60s ribosomal protein l14 (23 kDa, Protein from human)
- 60s ribosomal protein l3 (46 kDa, Protein from human)
- 60s ribosomal protein l39 (6 kDa, Protein from human)
- Protein sda1 homolog (80 kDa, Protein from human)
Human nuclear pre-60S ribosomal subunit (State I3) - Composite map
Resolution: 2.89 Å
EM Method: Single-particle
Fitted PDBs: 8fl4
Q-score: 0.494
Science (2023) 381 pp. eadh3892-eadh3892 [ DOI: doi:10.1126/science.adh3892 Pubmed: 37410842 ]
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Gtp-binding protein 4 (74 kDa, Protein from human)
- 60s ribosomal protein l26 (17 kDa, Protein from human)
- 28s rrna (1 MDa, RNA from human)
- Pescadillo homolog (68 kDa, Protein from human)
- 60s ribosomal protein l38 (8 kDa, Protein from human)
- 5s rrna (38 kDa, RNA from human)
- 60s ribosomal protein l36 (12 kDa, Protein from human)
- 60s ribosomal protein l15 (24 kDa, Protein from human)
- 60s ribosomal protein l27a (16 kDa, Protein from human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- Human nuclear pre-60s ribosomal subunit (state i3) (Complex from Homo sapiens)
- 60s ribosomal protein l18a (20 kDa, Protein from human)
- Zinc finger protein 593 (15 kDa, Protein from human)
- Protein llp homolog (15 kDa, Protein from human)
- 60s ribosomal protein l23a (17 kDa, Protein from human)
- 60s ribosomal protein l8 (28 kDa, Protein from human)
- 60s ribosomal protein l7-like 1 (29 kDa, Protein from human)
- 60s ribosomal protein l35a (12 kDa, Protein from human)
- Ribosomal biogenesis protein las1l (83 kDa, Protein from human)
- 60s ribosomal protein l37a (10 kDa, Protein from human)
- 60s ribosomal protein l37 (11 kDa, Protein from human)
- Testis-expressed protein 10 (105 kDa, Protein from human)
- Proline-, glutamic acid- and leucine-rich protein 1 (119 kDa, Protein from human)
- 60s ribosomal protein l30 (12 kDa, Protein from human)
- Potassium ion (39 Da, Ligand)
- 60s ribosomal protein l18 (21 kDa, Protein from human)
- 60s ribosomal protein l13 (24 kDa, Protein from human)
- 60s ribosomal protein l34 (13 kDa, Protein from human)
- Guanine nucleotide-binding protein-like 3 (62 kDa, Protein from human)
- 60s ribosomal protein l35 (14 kDa, Protein from human)
- Eukaryotic translation initiation factor 6 (26 kDa, Protein from human)
- Zinc ion (65 Da, Ligand)
- Coiled-coil domain-containing protein 86 (40 kDa, Protein from human)
- 60s ribosomal protein l10a (24 kDa, Protein from human)
- 60s ribosomal protein l23 (14 kDa, Protein from human)
- Nucleolar gtp-binding protein 2 (83 kDa, Protein from human)
- 60s ribosomal protein l7 (29 kDa, Protein from human)
- 60s ribosomal protein l32 (15 kDa, Protein from human)
- 60s ribosomal protein l22 (14 kDa, Protein from human)
- 60s ribosomal protein l11 (20 kDa, Protein from human)
- 60s ribosomal protein l7a (30 kDa, Protein from human)
- 60s ribosomal protein l9 (21 kDa, Protein from human)
- Wd repeat-containing protein 18 (47 kDa, Protein from human)
- 60s ribosomal protein l21 (18 kDa, Protein from human)
- 60s ribosomal protein l27 (15 kDa, Protein from human)
- 60s ribosomal protein l4 (47 kDa, Protein from human)
- Probable ribosome biogenesis protein rlp24 (19 kDa, Protein from human)
- 60s ribosomal protein l5 (34 kDa, Protein from human)
- 60s ribosomal protein l6 (32 kDa, Protein from human)
- Magnesium ion (24 Da, Ligand)
- 60s ribosomal protein l13a (23 kDa, Protein from human)
- 60s ribosomal protein l31 (14 kDa, Protein from human)
- 60s ribosomal protein l17 (21 kDa, Protein from human)
- Ribosome biogenesis protein nsa2 homolog (30 kDa, Protein from human)
- Ribosome biogenesis protein nop53 (54 kDa, Protein from human)
- 60s ribosomal protein l28 (15 kDa, Protein from human)
- Notchless protein homolog 1 (53 kDa, Protein from human)
- 60s ribosomal protein l12 (17 kDa, Protein from human)
- Mrna turnover protein 4 homolog (27 kDa, Protein from human)
- 5.8s rrna (50 kDa, RNA from human)
- 60s ribosomal protein l19 (23 kDa, Protein from human)
- 60s ribosomal protein l14 (23 kDa, Protein from human)
- 60s ribosomal protein l3 (46 kDa, Protein from human)
- 60s ribosomal protein l39 (6 kDa, Protein from human)
- Protein sda1 homolog (80 kDa, Protein from human)
Cryo-EM structure of the human SSU processome, state pre-A1
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7mq8
Q-score: 0.417
Singh S, Vanden Broeck A, Miller L, Chaker-Margot M, Klinge S
Science (2021) 373 pp. eabj5338-eabj5338 [ Pubmed: 34516797 DOI: doi:10.1126/science.abj5338 ]
- U3 small nucleolar rna-associated protein 18 homolog (62 kDa, Protein from Homo sapiens)
- Small subunit processome component 20 homolog (318 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s3a (30 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s15a (14 kDa, Protein from Homo sapiens)
- Krr1 small subunit processome component homolog (43 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s6 (28 kDa, Protein from Homo sapiens)
- Transducin beta-like protein 3 (89 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s7 (22 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s16 (16 kDa, Protein from Homo sapiens)
- 5'ets rrna (4 kDa, RNA from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 40s ribosomal protein s23 (15 kDa, Protein from Homo sapiens)
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s18 (12 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s28 (7 kDa, Protein from Homo sapiens)
- Nucleolar protein 58 (59 kDa, Protein from Homo sapiens)
- Nucleolar protein 14 (68 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 36 (105 kDa, Protein from Homo sapiens)
- Probable u3 small nucleolar rna-associated protein 11 (30 kDa, Protein from Homo sapiens)
- Something about silencing protein 10 (54 kDa, Protein from Homo sapiens)
- U3 small nucleolar rna-interacting protein 2 (51 kDa, Protein from Homo sapiens)
- Nucleolar protein 56 (66 kDa, Protein from Homo sapiens)
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Homo sapiens)
- Nucleolar protein 11 (73 kDa, Protein from Homo sapiens)
- Ribosomal rna-processing protein 7 homolog a (32 kDa, Protein from Homo sapiens)
- Nucleolar protein 10 (80 kDa, Protein from Homo sapiens)
- Nucleolar protein 6 (127 kDa, Protein from Homo sapiens)
- Human ssu processome (5 MDa, Complex from Homo sapiens)
- Protein aatf (63 kDa, Protein from Homo sapiens)
- Nucleolar complex protein 4 homolog (50 kDa, Protein from Homo sapiens)
- Rna cytidine acetyltransferase (115 kDa, Protein from Homo sapiens)
- U3 small nucleolar rna-associated protein 14 homolog a (88 kDa, Protein from Homo sapiens)
- Heat repeat-containing protein 1 (242 kDa, Protein from Homo sapiens)
- 5'ets rrna (1 MDa, RNA from Homo sapiens)
- 40s ribosomal protein s14 (16 kDa, Protein from Homo sapiens)
- Rna-binding protein pno1 (27 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 75 (94 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 46 (68 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s12 (14 kDa, Protein from Homo sapiens)
- Rrna 2'-o-methyltransferase fibrillarin (33 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s24 (15 kDa, Protein from Homo sapiens)
- Rrp12-like protein (73 kDa, Protein from Homo sapiens)
- Ribosome biogenesis protein bms1 homolog (145 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 40s ribosomal protein s9 (22 kDa, Protein from Homo sapiens)
- Unassigned peptides (15 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s5 (22 kDa, Protein from Homo sapiens)
- Periodic tryptophan protein 2 homolog (102 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 43 (74 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 3 (106 kDa, Protein from Homo sapiens)
- Nucleolar protein 7 (29 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s13 (17 kDa, Protein from Homo sapiens)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- Ddb1- and cul4-associated factor 13 (51 kDa, Protein from Homo sapiens)
- U3 small nucleolar rna-associated protein 15 homolog (58 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s11 (18 kDa, Protein from Homo sapiens)
- Rrna-processing protein fcf1 homolog (23 kDa, Protein from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- Neuroguidin (35 kDa, Protein from Homo sapiens)
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Homo sapiens)
- Ribosomal rna small subunit methyltransferase nep1 (26 kDa, Protein from Homo sapiens)
- Bystin (33 kDa, Protein from Homo sapiens)
- 18s rrna (602 kDa, RNA from Homo sapiens)
- U3 small nucleolar rna-associated protein 4 homolog (76 kDa, Protein from Homo sapiens)
- U3 small nucleolar ribonucleoprotein protein mpp10 (78 kDa, Protein from Homo sapiens)
- Uncharacterized protein c1orf131 (32 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1 (14 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s8 (24 kDa, Protein from Homo sapiens)
- U3 small nucleolar rna-associated protein 6 homolog (70 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s17 (15 kDa, Protein from Homo sapiens)
- Ubiquitin-40s ribosomal protein s27a (18 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s4, x isoform (29 kDa, Protein from Homo sapiens)
- Deoxynucleotidyltransferase terminal-interacting protein 2 (84 kDa, Protein from Homo sapiens)
- U3 snorna (70 kDa, RNA from Homo sapiens)
- 40s ribosomal protein s27 (9 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human SSU processome, state pre-A1*
Resolution: 3.87 Å
EM Method: Single-particle
Fitted PDBs: 7mq9
Q-score: 0.357
Singh S, Vanden Broeck A, Miller L, Chaker-Margot M, Klinge S
Science (2021) 373 pp. eabj5338-eabj5338 [ Pubmed: 34516797 DOI: doi:10.1126/science.abj5338 ]
- U3 small nucleolar rna-associated protein 18 homolog (62 kDa, Protein from Homo sapiens)
- Small subunit processome component 20 homolog (318 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s3a (30 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s15a (14 kDa, Protein from Homo sapiens)
- Krr1 small subunit processome component homolog (43 kDa, Protein from Homo sapiens)
- 18s rrna (603 kDa, RNA from Homo sapiens)
- 40s ribosomal protein s6 (28 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s4 x isoform (29 kDa, Protein from Homo sapiens)
- Transducin beta-like protein 3 (89 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s7 (22 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s16 (16 kDa, Protein from Homo sapiens)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- 40s ribosomal protein s23 (15 kDa, Protein from Homo sapiens)
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s18 (12 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s28 (7 kDa, Protein from Homo sapiens)
- Nucleolar protein 58 (59 kDa, Protein from Homo sapiens)
- Nucleolar protein 14 (68 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 36 (105 kDa, Protein from Homo sapiens)
- Probable u3 small nucleolar rna-associated protein 11 (30 kDa, Protein from Homo sapiens)
- Something about silencing protein 10 (54 kDa, Protein from Homo sapiens)
- U3 small nucleolar rna-interacting protein 2 (51 kDa, Protein from Homo sapiens)
- Nucleolar protein 56 (66 kDa, Protein from Homo sapiens)
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Homo sapiens)
- Ribosome biogenesis protein bms1 homolog (146 kDa, Protein from Homo sapiens)
- Nucleolar protein 10 (80 kDa, Protein from Homo sapiens)
- Ribosomal rna-processing protein 7 homolog a (32 kDa, Protein from Homo sapiens)
- Nucleolar protein 6 (127 kDa, Protein from Homo sapiens)
- Human ssu processome (5 MDa, Complex from Homo sapiens)
- Protein aatf (63 kDa, Protein from Homo sapiens)
- Nucleolar complex protein 4 homolog (50 kDa, Protein from Homo sapiens)
- Rna cytidine acetyltransferase (115 kDa, Protein from Homo sapiens)
- U3 small nucleolar rna-associated protein 14 homolog a (88 kDa, Protein from Homo sapiens)
- Heat repeat-containing protein 1 (242 kDa, Protein from Homo sapiens)
- 5'ets rrna (1 MDa, RNA from Homo sapiens)
- 40s ribosomal protein s14 (16 kDa, Protein from Homo sapiens)
- Rna-binding protein pno1 (27 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 75 (94 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 46 (68 kDa, Protein from Homo sapiens)
- Zinc ion (65 Da, Ligand)
- 40s ribosomal protein s12 (14 kDa, Protein from Homo sapiens)
- Rrna 2'-o-methyltransferase fibrillarin (33 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s24 (15 kDa, Protein from Homo sapiens)
- Rrp12-like protein (73 kDa, Protein from Homo sapiens)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 40s ribosomal protein s9 (22 kDa, Protein from Homo sapiens)
- Unassigned peptides (15 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s5 (22 kDa, Protein from Homo sapiens)
- Periodic tryptophan protein 2 homolog (102 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 43 (74 kDa, Protein from Homo sapiens)
- Wd repeat-containing protein 3 (106 kDa, Protein from Homo sapiens)
- Nucleolar protein 7 (29 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s13 (17 kDa, Protein from Homo sapiens)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- Ddb1- and cul4-associated factor 13 (51 kDa, Protein from Homo sapiens)
- U3 small nucleolar rna-associated protein 15 homolog (58 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s11 (18 kDa, Protein from Homo sapiens)
- Rrna-processing protein fcf1 homolog (23 kDa, Protein from Homo sapiens)
- 5' ets rrna (7 kDa, RNA from Homo sapiens)
- Magnesium ion (24 Da, Ligand)
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Homo sapiens)
- Nucleolar protein 11 (75 kDa, Protein from Homo sapiens)
- Ribosomal rna small subunit methyltransferase nep1 (26 kDa, Protein from Homo sapiens)
- Bystin (33 kDa, Protein from Homo sapiens)
- U3 small nucleolar rna-associated protein 4 homolog (76 kDa, Protein from Homo sapiens)
- U3 small nucleolar ribonucleoprotein protein mpp10 (78 kDa, Protein from Homo sapiens)
- Uncharacterized protein c1orf131 (32 kDa, Protein from Homo sapiens)
- Nhp2-like protein 1 (14 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s8 (24 kDa, Protein from Homo sapiens)
- U3 small nucleolar rna-associated protein 6 homolog (70 kDa, Protein from Homo sapiens)
- 40s ribosomal protein s17 (15 kDa, Protein from Homo sapiens)
- Ubiquitin-40s ribosomal protein s27a (18 kDa, Protein from Homo sapiens)
- Deoxynucleotidyltransferase terminal-interacting protein 2 (84 kDa, Protein from Homo sapiens)
- U3 snorna (70 kDa, RNA from Homo sapiens)
- 40s ribosomal protein s27 (9 kDa, Protein from Homo sapiens)
Cryo-EM structure of the human SSU processome, state post-A1
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7mqa
Q-score: 0.526
Singh S, Vanden Broeck A, Miller L, Chaker-Margot M, Klinge S
Science (2021) 373 pp. eabj5338-eabj5338 [ Pubmed: 34516797 DOI: doi:10.1126/science.abj5338 ]
- 40s ribosomal protein s11 (18 kDa, Protein from Human)
- 40s ribosomal protein s9 (22 kDa, Protein from Human)
- Wd repeat-containing protein 3 (106 kDa, Protein from Human)
- Wd repeat-containing protein 36 (105 kDa, Protein from Human)
- 40s ribosomal protein s27 (9 kDa, Protein from Human)
- 40s ribosomal protein s5 (22 kDa, Protein from Human)
- Heat repeat-containing protein 1 (242 kDa, Protein from Human)
- Nucleolar protein 56 (66 kDa, Protein from Human)
- 5'ets rrna (1 MDa, RNA from Human)
- 40s ribosomal protein s18 (12 kDa, Protein from Human)
- 40s ribosomal protein s7 (22 kDa, Protein from Human)
- 40s ribosomal protein s17 (15 kDa, Protein from Human)
- Rna-binding protein pno1 (27 kDa, Protein from Human)
- 40s ribosomal protein s4, x isoform (29 kDa, Protein from Human)
- Guanosine-5'-triphosphate (523 Da, Ligand)
- U3 small nucleolar rna-associated protein 15 homolog (58 kDa, Protein from Human)
- Probable atp-dependent rna helicase dhx37 (129 kDa, Protein from Human)
- 40s ribosomal protein s16 (16 kDa, Protein from Human)
- Periodic tryptophan protein 2 homolog (102 kDa, Protein from Human)
- Ubiquitin-40s ribosomal protein s27a (18 kDa, Protein from Human)
- 40s ribosomal protein s24 (15 kDa, Protein from Human)
- Nhp2-like protein 1 (14 kDa, Protein from Human)
- Human ssu processome (5 MDa, Complex from Homo sapiens)
- Wd repeat-containing protein 43 (74 kDa, Protein from Human)
- Rrna 2'-o-methyltransferase fibrillarin (33 kDa, Protein from Human)
- Nucleolar protein 14 (68 kDa, Protein from Human)
- 40s ribosomal protein s13 (17 kDa, Protein from Human)
- Exosome component 10 (100 kDa, Protein from Human)
- U3 small nucleolar rna-interacting protein 2 (51 kDa, Protein from Human)
- Zinc ion (65 Da, Ligand)
- Ddb1- and cul4-associated factor 13 (51 kDa, Protein from Human)
- Wd repeat-containing protein 46 (68 kDa, Protein from Human)
- U3 small nucleolar ribonucleoprotein protein imp3 (21 kDa, Protein from Human)
- Adenosine-5'-triphosphate (507 Da, Ligand)
- 40s ribosomal protein s15a (14 kDa, Protein from Human)
- 40s ribosomal protein s12 (14 kDa, Protein from Human)
- 40s ribosomal protein s19 (16 kDa, Protein from Human)
- S-adenosyl-l-homocysteine (384 Da, Ligand)
- 18s rrna (604 kDa, RNA from Human)
- U3 small nucleolar ribonucleoprotein protein mpp10 (78 kDa, Protein from Human)
- 40s ribosomal protein s14 (16 kDa, Protein from Human)
- Nucleolar protein 6 (127 kDa, Protein from Human)
- 40s ribosomal protein s23 (15 kDa, Protein from Human)
- Rrp12-like protein (73 kDa, Protein from Human)
- U3 snorna (70 kDa, RNA from Human)
- Probable u3 small nucleolar rna-associated protein 11 (30 kDa, Protein from Human)
- Magnesium ion (24 Da, Ligand)
- Active regulator of sirt1 (15 kDa, Protein from Human)
- 40s ribosomal protein s28 (7 kDa, Protein from Human)
- Deoxynucleotidyltransferase terminal-interacting protein 2 (84 kDa, Protein from Human)
- Nucleolar protein 11 (73 kDa, Protein from Human)
- Small subunit processome component 20 homolog (318 kDa, Protein from Human)
- Nucleolar protein 7 (29 kDa, Protein from Human)
- U3 small nucleolar ribonucleoprotein protein imp4 (33 kDa, Protein from Human)
- U3 small nucleolar rna-associated protein 4 homolog (76 kDa, Protein from Human)
- Probable dimethyladenosine transferase (35 kDa, Protein from Human)
- Ribosomal rna-processing protein 7 homolog a (32 kDa, Protein from Human)
- Ribosome biogenesis protein bms1 homolog (146 kDa, Protein from Human)
- U3 small nucleolar rna-associated protein 14 homolog a (88 kDa, Protein from Human)
- U3 small nucleolar rna-associated protein 6 homolog (70 kDa, Protein from Human)
- Rrna-processing protein fcf1 homolog (23 kDa, Protein from Human)
- Wd repeat-containing protein 75 (94 kDa, Protein from Human)
- Transducin beta-like protein 3 (89 kDa, Protein from Human)
- U3 small nucleolar rna-associated protein 18 homolog (62 kDa, Protein from Human)
- 40s ribosomal protein s8 (24 kDa, Protein from Human)
- Something about silencing protein 10 (54 kDa, Protein from Human)
- Nucleolar protein 58 (59 kDa, Protein from Human)
- Rna 3'-terminal phosphate cyclase-like protein (40 kDa, Protein from Human)
- Unassigned peptides (19 kDa, Protein from Human)
- 40s ribosomal protein s3a (30 kDa, Protein from Human)
- 40s ribosomal protein s6 (28 kDa, Protein from Human)
- Ribosomal rna small subunit methyltransferase nep1 (26 kDa, Protein from Human)
- Nucleolar complex protein 4 homolog (50 kDa, Protein from Human)
- Bystin (33 kDa, Protein from Human)