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EMBL

was set up in 1974 as Europe’s flagship laboratory for the life sciences – an intergovernmental organisation with more than 80 independent research groups covering the spectrum of molecular biology:

Research:

perform basic research in molecular biology

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offer vital services to scientists in the member states

Training

scientists, students and visitors at all levels

Transfer

and development of technology

Develop

new instruments and methods

Integrating

life science research in Europe

More about EMBL

Six sites

represent EMBL in Europe.

Barcelona

Tissue biology and disease modelling

Grenoble

Structural biology

Hamburg

Structural biology

Heidelberg

Main laboratory

Hinxton

EMBL-EBI: European Bioinformatics Institute

Rome

Epigenetics and neurobiology

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sample_name:"DNA-directed RNA polymerase III subunit RPC6" AND author_name:"Hagen WJH"

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EMD-10595

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Cryo-EM structure of the RNA Polymerase III-Maf1 complex

Release date: Feb. 19, 2020
Resolution: 3.25 Å
EM Method: Single-particle
Fitted PDBs: 6tut
Q-score: 0.49

Vorlander MK, Baudin F, Moir RD, Wetzel R, Hagen WJH, Willis IM, Muller CW

Nat Struct Mol Biol (2020) 27 pp. 229-232 [ DOI: doi:10.1038/s41594-020-0383-y Pubmed: 32066962 ]

Sample:

Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii (Complex from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Repressor of rna polymerase iii transcription maf1,repressor of rna polymerase iii transcription maf1 (30 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae S288C)
Repressor of rna polymerase iii transcription maf1 (Complex from Saccharomyces cerevisiae S288C)
Rna polymerase iii - maf1 complex (720 kDa, Complex)
Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae S288C)

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EMD-4180

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RNA Polymerase III open complex

Release date: Jan. 17, 2018
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 6f40
Q-score: 0.412

Vorlander MK, Khatter H, Wetzel R, Hagen WJH, Muller CW

Nature (2018) 553 pp. 295-300 [ DOI: doi:10.1038/nature25440 Pubmed: 29345638 ]

Sample:

Rna polymerase iii (Complex from Saccharomyces cerevisiae)
Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor iiib (Complex from Saccharomyces cerevisiae)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor tfiiib component b'' (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor iiib 70 kda subunit (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna polymerase iii open complex (893 kDa, Complex)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Tata-box-binding protein (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleic acids (Complex from Saccharomyces cerevisiae)
Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Non-template dna (24 kDa, DNA from Saccharomyces cerevisiae)
Template dna (25 kDa, DNA from Saccharomyces cerevisiae)
Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMD-4181

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RNA Polymerase III initially transcribing complex

Release date: Jan. 17, 2018
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6f41
Q-score: 0.342

Vorlander MK, Khatter H, Wetzel R, Hagen WJH, Muller CW

Nature (2018) 553 pp. 295-300 [ DOI: doi:10.1038/nature25440 Pubmed: 29345638 ]

Sample:

Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factors (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Nucleic acids (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Non-template dna (24 kDa, DNA from Saccharomyces cerevisiae S288c)
Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor tfiiib component b'' (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor iiib 70 kda subunit (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Template-dna (25 kDa, DNA from Saccharomyces cerevisiae S288c)
Rna polymerase iii (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Tata-box-binding protein (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna polymerase iii initially transcribing complex (893 kDa, Complex)
Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna (1 kDa, RNA from Saccharomyces cerevisiae S288c)
Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMD-4182

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RNA Polymerase III closed complex CC1

Release date: Jan. 17, 2018
Resolution: 5.5 Å
EM Method: Single-particle
Fitted PDBs: 6f42
Q-score: 0.194

Vorlander MK, Khatter H, Wetzel R, Hagen WJH, Muller CW

Nature (2018) 553 pp. 295-300 [ DOI: doi:10.1038/nature25440 Pubmed: 29345638 ]

Sample:

Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Non-template dna (24 kDa, DNA from Saccharomyces cerevisiae)
Nucleic acids (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor tfiiib component b'' (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Template dna (25 kDa, DNA from Saccharomyces cerevisiae)
Transcription factor iiib 70 kda subunit (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna polymerase (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna polymerase iii closed complex cc1 (893 kDa, Complex)
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Tata-box-binding protein (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Cc1 (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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EMD-4183

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RNA Polymerase III closed complex CC2

Release date: Jan. 17, 2018
Resolution: 4.2 Å
EM Method: Single-particle
Fitted PDBs: 6f44
Q-score: 0.303

Vorlander MK, Khatter H, Wetzel R, Hagen WJH, Muller CW

Nature (2018) 553 pp. 295-300 [ DOI: doi:10.1038/nature25440 Pubmed: 29345638 ]

Sample:

Rna polymerase iii closed complex cc2 (893 kDa, Complex)
Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Non-template dna (24 kDa, DNA from Saccharomyces cerevisiae)
Nucleic acids (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor tfiiib component b'' (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Template dna (25 kDa, DNA from Saccharomyces cerevisiae)
Transcription factor iiib 70 kda subunit (67 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Rna polymerase iii (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Transcription factor iiib (Complex from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Tata-box-binding protein (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc4 (46 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))
Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae (strain ATCC 204508 / S288c))

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