Resolution: 2.8 Å
EM Method: Single-particle
Fitted PDBs: 8re4
Q-score: 0.47
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(p*gp*cp*cp*gp*c)-3') (1 kDa, RNA from Klebsiella oxytoca)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna (47-mer) (14 kDa, DNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Rna polymerase sigma-54 factor (42 kDa, Protein from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Dna (50-mer) (15 kDa, DNA from Klebsiella oxytoca)
- Rna polymerase-sigma54 initial transcribing complex - 5nt pre-translocated complex (Complex from Escherichia coli K-12)
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8rea
Q-score: 0.427
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Dna (51-mer) (15 kDa, DNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(p*gp*cp*cp*gp*c)-3') (1 kDa, RNA from Klebsiella oxytoca)
- Rna polymerase-sigma54 initial transcribing complex - 5nt post-translocated complex (Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna (44-mer) (13 kDa, DNA from Klebsiella oxytoca)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Rna polymerase sigma-54 factor (42 kDa, Protein from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 6nt complex
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8reb
Q-score: 0.108
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(p*gp*cp*cp*gp*cp*g)-3') (1 kDa, RNA from Klebsiella oxytoca)
- Dna (43-mer) (13 kDa, DNA from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Rna polymerase-sigma54 initial transcribing complex - 5nt post-translocated complex (Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Rna polymerase sigma-54 factor (39 kDa, Protein from Klebsiella oxytoca)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Dna (52-mer) (16 kDa, DNA from Klebsiella oxytoca)
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 7nt complex
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 8rec
Q-score: 0.406
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
- Dna (46-mer) (14 kDa, DNA from Klebsiella oxytoca)
- Rna polymerase-sigma54 initial transcribing complex - 7nt complex (Complex from Escherichia coli K-12)
- Dna (51-mer) (15 kDa, DNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Rna polymerase sigma-54 factor (39 kDa, Protein from Klebsiella oxytoca)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Rna (5'-r(p*gp*cp*cp*gp*cp*gp*a)-3') (2 kDa, RNA from Klebsiella oxytoca)
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 8nt complex
Resolution: 3.9 Å
EM Method: Single-particle
Fitted PDBs: 8red
Q-score: 0.387
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
- Dna (46-mer) (14 kDa, DNA from Klebsiella oxytoca)
- Rna polymerase sigma-54 factor (36 kDa, Protein from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Rna polymerase-sigma54 initial transcribing complex - 8nt complex (Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Rna (5'-r(p*gp*cp*cp*gp*cp*gp*ap*u)-3') (2 kDa, RNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Dna (51-mer) (15 kDa, DNA from Klebsiella oxytoca)
- Zinc ion (65 Da, Ligand)
Cryo-EM structure of bacterial RNA polymerase-sigma54 initial transcribing complex - 9nt complex
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 8ree
Q-score: 0.136
Gao F, Ye F, Zhang B, Cronin N, Buck M, Zhang X
PNAS (2024) 121 pp. e2309670120-e2309670120 [ Pubmed: 38170755 DOI: doi:10.1073/pnas.2309670120 ]
- Rna polymerase-sigma54 initial transcribing complex - 9nt complex (Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (35 kDa, Protein from Escherichia coli K-12)
- Rna (5'-r(p*gp*cp*cp*gp*cp*gp*ap*up*c)-3') (2 kDa, RNA from Klebsiella oxytoca)
- Dna (45-mer) (13 kDa, DNA from Klebsiella oxytoca)
- Dna (49-mer) (15 kDa, DNA from Klebsiella oxytoca)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit beta' (152 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (8 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Rna polymerase sigma-54 factor (36 kDa, Protein from Klebsiella oxytoca)