RNA polymerase at U-rich pause bound to non-regulatory RNA - inactive, open clamp state
Resolution: 4.06 Å
EM Method: Single-particle
Fitted PDBs: 8ac1
Q-score: 0.334
Dey S, Batisse C, Shukla J, Webster MW, Takacs M, Saint-Andre C, Weixlbaumer A
Mol Cell (2022) 82 pp. 3885- [ DOI: doi:10.1016/j.molcel.2022.09.020 Pubmed: 36220101 ]
- Dna non-template strand (91 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Non-regulatory rna (39 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Escherichia coli rna polymerase non-regulatory rna complex - inactive, open clamp state (435 kDa, Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna template strand (91 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8aby
Q-score: 0.422
Dey S, Batisse C, Shukla J, Webster MW, Takacs M, Saint-Andre C, Weixlbaumer A
Mol Cell (2022) 82 pp. 3885- [ DOI: doi:10.1016/j.molcel.2022.09.020 Pubmed: 36220101 ]
- Template dna (13 kDa, DNA from Escherichia coli K-12)
- Rna putl (30 kDa, RNA from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Escherichia coli rna polymerase and in vitro transcribed and purified putl rna complex - pause prone closed clamp state (435 kDa, Complex from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Non-template dna (13 kDa, DNA from Escherichia coli K-12)
RNA polymerase at U-rich pause bound to non-regulatory RNA - pause prone, closed clamp state
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 8abz
Q-score: 0.438
Dey S, Batisse C, Shukla J, Webster MW, Takacs M, Saint-Andre C, Weixlbaumer A
Mol Cell (2022) 82 pp. 3885- [ DOI: doi:10.1016/j.molcel.2022.09.020 Pubmed: 36220101 ]
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Non-regulatory rna (39 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Zinc ion (65 Da, Ligand)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Non-template dna (91 kDa, DNA from Escherichia coli K-12)
- Escherichia coli rna polymerase non-regulatory rna complex - pause prone, closed clamp state (435 kDa, Complex from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Template dna (91 kDa, DNA from Escherichia coli K-12)
RNA polymerase at U-rich pause bound to regulatory RNA putL - active, closed clamp state
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8ac0
Q-score: 0.35
Dey S, Batisse C, Shukla J, Webster MW, Takacs M, Saint-Andre C, Weixlbaumer A
Mol Cell (2022) 82 pp. 3885- [ DOI: doi:10.1016/j.molcel.2022.09.020 Pubmed: 36220101 ]
- Escherichia coli rna polymerase putl complex - active closed clamp state (435 kDa, Complex from Escherichia coli K-12)
- Dna non-template strand (82 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Dna template strand (82 kDa, DNA from Escherichia coli)
- Putl rna (30 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli BL21)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
RNA polymerase- post-terminated, open clamp state
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 8ac2
Q-score: 0.361
Dey S, Batisse C, Shukla J, Webster MW, Takacs M, Saint-Andre C, Weixlbaumer A
Mol Cell (2022) 82 pp. 3885- [ DOI: doi:10.1016/j.molcel.2022.09.020 Pubmed: 36220101 ]
- Dna non-template strand (91 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Dna template strand (91 kDa, DNA from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Escherichia coli rna polymerase post-terminated complex - open clamp state (435 kDa, Complex from Escherichia coli K-12)
RNA polymerase at U-rich pause bound to regulatory RNA putL - inactive, open clamp state
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 8acp
Q-score: 0.27
Dey S, Batisse C, Shukla J, Webster MW, Takacs M, Saint-Andre C, Weixlbaumer A
Mol Cell (2022) 82 pp. 3885- [ DOI: doi:10.1016/j.molcel.2022.09.020 Pubmed: 36220101 ]
- Non-template dna (82 kDa, DNA from Escherichia coli)
- Template dna (82 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Putl rna (30 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Escherichia coli rna polymerase putl complex - inactive open clamp state (435 kDa, Complex from Escherichia coli K-12)
RNA polymerase at U-rich pause bound to RNA putL triple mutant - pause prone, closed clamp state
Resolution: 4.1 Å
EM Method: Single-particle
Fitted PDBs: 8ad1
Q-score: 0.337
Dey S, Batisse C, Shukla J, Webster MW, Takacs M, Saint-Andre C, Weixlbaumer A
Mol Cell (2022) 82 pp. 3885- [ DOI: doi:10.1016/j.molcel.2022.09.020 Pubmed: 36220101 ]
- Rna polymerase sigma factor rpod (70 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli K-12)
- Non-template dna (82 kDa, DNA from Escherichia coli K-12)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli K-12)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli K-12)
- Escherichia coli rna polymerase putl triple mutant complex - pause prone, closed clamp state (435 MDa, Complex from Escherichia coli K-12)
- Template dna (82 kDa, DNA from Escherichia coli K-12)
- Magnesium ion (24 Da, Ligand)
- Zinc ion (65 Da, Ligand)
- Rna putl triple mutant (29 kDa, RNA from Escherichia coli K-12)
Resolution: 4.5 Å
EM Method: Single-particle
Fitted PDBs: 7py0
Q-score: 0.185
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Ntdna (12 kDa, DNA from Escherichia coli)
- Nusg elongation complex in more-swiveled conformation (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Tdna (11 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
CryoEM structure of E.coli RNA polymerase elongation complex bound to NusG (the consensus NusG-EC)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7py1
Q-score: 0.343
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Ntdna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Tdna (11 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Transcription termination/antitermination protein nusg (20 kDa, Protein from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Nusg elongation complex (consensus) (Complex from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
CryoEM structure of E.coli RNA polymerase elongation complex bound to NusA (the consensus NusA-EC)
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 7py3
Q-score: 0.365
Zhu C, Guo X, Dumas P, Takacs M, Abdelkareem M, Vanden Broeck A, Saint-Andre C, Papai G, Crucifix C, Ortiz J, Weixlbaumer A
Nat Commun (2022) 13 pp. 1546-1546 [ Pubmed: 35318334 DOI: doi:10.1038/s41467-022-29148-0 ]
- Ntdna (12 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta (150 kDa, Protein from Escherichia coli)
- Tdna (11 kDa, DNA from Escherichia coli)
- Dna-directed rna polymerase subunit alpha (36 kDa, Protein from Escherichia coli)
- Cryoem structure of e.coli rna polymerase elongation complex bound to nusa (the consensus nusa-ec) (Complex from Escherichia coli)
- Rna (4 kDa, RNA from Escherichia coli)
- Dna-directed rna polymerase subunit beta' (155 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- Dna-directed rna polymerase subunit omega (10 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- Transcription termination/antitermination protein nusa (54 kDa, Protein from Escherichia coli MS 115-1)