Electron Microscopy Data Bank

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Structure of yeast RNA Polymerase III-Ty1 integrase complex at 2.9 A (focus subunit C11 terminal Zn-ribbon in the funnel pore).

Release date: April 5, 2023
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7z30
Q-score: 0.464

Nguyen PQ, Huecas S, Asif-Laidin A, Plaza-Pegueroles A, Capuzzi B, Palmic N, Conesa C, Acker J, Reguera J, Lesage P, Fernandez-Tornero C

Nat Commun (2023) 14 pp. 1729-1729 [ Pubmed: 36977686 DOI: doi:10.1038/s41467-023-37109-4 ]

Sample:

Dna-directed rna polymerase iii subunit rpc7 (27 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc5 (8 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc3 (74 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc6 (36 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc5 (32 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc2 (17 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i and iii subunit rpac1 (37 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc4 (7 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc1 (25 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc2 (129 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc8 (24 kDa, Protein from Saccharomyces cerevisiae S288C)
Zinc ion (65 Da, Ligand)
Dna-directed rna polymerase iii subunit rpc1 (162 kDa, Protein from Saccharomyces cerevisiae S288C)
Unknown rna polymerase iii chain (1 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i, ii, and iii subunit rpabc3 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Water (18 Da, Ligand)
Cryo-em map of the crosslinked rna polymerase iii-ty1 integrase complex at 2.9 a (focussing on c11 subunit) (Complex from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerases i and iii subunit rpac2 (16 kDa, Protein from Saccharomyces cerevisiae S288C)
Magnesium ion (24 Da, Ligand)
Dna-directed rna polymerase iii subunit rpc9 (18 kDa, Protein from Saccharomyces cerevisiae S288C)
Integrase (71 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc4 (42 kDa, Protein from Saccharomyces cerevisiae S288C)
Dna-directed rna polymerase iii subunit rpc10 (12 kDa, Protein from Saccharomyces cerevisiae S288C)

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