Takada H, Paternoga H, Fujiwara K, Nakamoto JA, Park EN, Dimitrova-Paternoga L, Beckert B, Saarma M, Tenson T, Buskirk AR, Atkinson GC, Chiba S, Wilson DN, Hauryliuk V
Nucleic Acids Res (2024) [ Pubmed: 38811035 DOI: doi:10.1093/nar/gkae399 ]
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l21 (11 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Large ribosomal subunit protein ul6 (19 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l2 (30 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l34 (5 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l22 (12 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l16 (16 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l4 (22 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal subunit with stalled p-trna-nascent chain, bound by ylmh (Complex from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l29 (7 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Potassium ion (39 Da, Ligand)
- Chloramphenicol (323 Da, Ligand)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Zinc ion (65 Da, Ligand)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Large ribosomal subunit protein bl27 (10 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Putative rna-binding protein ylmh (30 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l17 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l35 (7 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Water (18 Da, Ligand)
- 50s ribosomal protein l3 (22 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- P-trna (24 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- Magnesium ion (24 Da, Ligand)
- Nascent chain (358 Da, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l5 (20 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l31 (7 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l33 1 (5 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 23s rrna (949 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 5s rrna (36 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l28 (6 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l13 (16 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l24 (11 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
YlmH bound to stalled 50S subunits with RqcH and PtRNA
Takada H, Paternoga H, Fujiwara K, Nakamoto JA, Park EN, Dimitrova-Paternoga L, Beckert B, Saarma M, Tenson T, Buskirk AR, Atkinson GC, Chiba S, Wilson DN, Hauryliuk V
Nucleic Acids Res (2024) [ Pubmed: 38811035 DOI: doi:10.1093/nar/gkae399 ]
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l21 (11 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Large ribosomal subunit protein ul6 (19 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Rqc2 homolog rqch (65 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l2 (30 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l34 (5 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l22 (12 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l16 (16 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l4 (22 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal subunit with stalled p-trna-nascent chain, bound by ylmh (Complex from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l29 (7 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Potassium ion (39 Da, Ligand)
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Large ribosomal subunit protein bl27 (10 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Putative rna-binding protein ylmh (30 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l17 (13 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l35 (7 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l3 (22 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- P-trna (24 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l5 (20 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l31 (7 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l33 1 (5 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 23s rrna (949 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 5s rrna (36 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l28 (6 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l13 (16 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l24 (11 kDa, Protein from Bacillus subtilis subsp. subtilis str. 168)
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7as9
Q-score: 0.3
Crowe-McAuliffe C, Takada H, Murina V, Polte C, Kasvandik S, Tenson T, Ignatova Z, Atkinson GC, Wilson DN, Hauryliuk V
Mol Cell (2021) 81 pp. 115- [ DOI: doi:10.1016/j.molcel.2020.11.002 Pubmed: 33259810 ]
- Trna-ala-1-1 (24 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l2 (30 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l20 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l10 (18 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l17 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l22 (12 kDa, Protein from Bacillus subtilis (strain 168))
- 5s rrna (38 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l4 (22 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l21 (11 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l27 (10 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l34 (5 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l29 (7 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l33 1 (5 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal subunit in complex with p-trna, and yabo/rqcp (Complex from Bacillus subtilis (strain 168))
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l16 (16 kDa, Protein from Bacillus subtilis (strain 168))
- Rqc2 homolog rqch (68 kDa, Protein from Bacillus subtilis (strain 168))
- Rqch (Complex from Bacillus subtilis (strain 168))
- 50s ribosomal protein l28 (6 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l35 (7 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l11 (14 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l24 (11 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l13 (16 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l23 (10 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis (strain 168))
- 23s rrna (949 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l30 (6 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l6 (19 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l3 (22 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l5 (20 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal subunit in complex with rqch, p-trna, and yabo/rqcp (1 MDa, Complex)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7as8
Q-score: 0.497
Crowe-McAuliffe C, Takada H, Murina V, Polte C, Kasvandik S, Tenson T, Ignatova Z, Atkinson GC, Wilson DN, Hauryliuk V
Mol Cell (2021) 81 pp. 115- [ DOI: doi:10.1016/j.molcel.2020.11.002 Pubmed: 33259810 ]
- Trna-ala-1-1 (24 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l2 (30 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l20 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l10 (18 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l17 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 5s rrna (38 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l22 (12 kDa, Protein from Bacillus subtilis (strain 168))
- Uncharacterized protein yabo (9 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l4 (22 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l21 (11 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l27 (10 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l36 (4 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l34 (5 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l15 (15 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l29 (7 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l33 1 (5 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal subunit in complex with p-trna, and yabo/rqcp (Complex from Bacillus subtilis (strain 168))
- 50s ribosomal protein l18 (12 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l16 (16 kDa, Protein from Bacillus subtilis (strain 168))
- Rqc2 homolog rqch (68 kDa, Protein from Bacillus subtilis (strain 168))
- Rqch (Complex from Bacillus subtilis (strain 168))
- 50s ribosomal protein l28 (6 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l35 (7 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l11 (14 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l14 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l19 (13 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l24 (11 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l13 (16 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l23 (10 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l32 (6 kDa, Protein from Bacillus subtilis (strain 168))
- 23s rrna (949 kDa, RNA from Bacillus subtilis subsp. subtilis str. 168)
- 50s ribosomal protein l30 (6 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l6 (19 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l3 (22 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal protein l5 (20 kDa, Protein from Bacillus subtilis (strain 168))
- 50s ribosomal subunit in complex with rqch, p-trna, and yabo/rqcp (1 MDa, Complex)