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Elongation factor G-ribosome complex captures in the absence of inhibitors.
Resolution: 3.8 Å
EM Method: Single-particle
Fitted PDBs: 5ot7
Q-score: 0.344
Mace K, Giudice E, Chat S, Gillet R
Nucleic Acids Res (2018) 46 pp. 3211-3217 [ Pubmed: 29408956 DOI: doi:10.1093/nar/gky081 ]
- Trna phe (Complex from Escherichia coli)
- 23s ribosomal rna (943 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l3 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l5 (20 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s5 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l28 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Ribosomal protein bl12 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l25 (20 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l16 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l9 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s19 (9 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l24 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s15 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l35 (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Elongation factor g (Complex from Thermus thermophilus)
- Ef-g bound to 70s ribosome (Complex)
- 30s ribosomal protein s17 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l29 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l33 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 70s ribosome (Complex from Thermus thermophilus HB8)
- Mrna (4 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s9 (14 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l27 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l2 (30 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s14 type z (7 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Mrna (Complex from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s4 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l13 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein thx (3 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l6 (19 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l22 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l4 (23 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Elongation factor g (76 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s8 (15 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l18 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l15 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Ribosomal protein ul11 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l19 (16 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Trna-phe (24 kDa, RNA from Escherichia coli)
- Ribosomal protein ul10 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l14 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l30 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s20 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s13 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s18 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l17 (13 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s2 (27 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 16s ribosomal rna (489 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s16 (9 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l23 (10 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l36 (4 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s11 (12 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l31 (8 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l1 (24 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l32 (6 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s7 (17 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s6 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 30s ribosomal protein s3 (22 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l21 (11 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 5s ribosomal rna (38 kDa, RNA from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- 50s ribosomal protein l34 (5 kDa, Protein from Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579))
- Guanosine-5'-diphosphate (443 Da, Ligand)