Electron Microscopy Data Bank

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sample_name:"50S ribosomal protein L33" AND author_name:"Paleskava A" AND author_name:"Stark H"

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EMD-4121

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Structure of the 70S ribosome with SECIS-mRNA and P-site tRNA (Initial complex, IC)

Release date: Nov. 23, 2016
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 5lza
Q-score: 0.407

Fischer N, Neumann P, Bock LV, Maracci C, Wang Z, Paleskava A, Konevega AL, Schroder GF, Grubmuller H, Ficner R, Rodnina MV, Stark H

Nature (2016) 540 pp. 80-85 [ DOI: doi:10.1038/nature20560 Pubmed: 27842381 ]

Sample:

30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli)
Fmet-trnafmet (24 kDa, RNA from Escherichia coli)
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
16s ribosomal rna (498 kDa, RNA from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
Secis mrna (15 kDa, RNA from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli)
Initial complex, e. coli ribosome 70s-fmet-trnafmet-secis mrna complex (ic) (2 MDa, Complex from Escherichia coli)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)

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EMD-4122

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Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the initial binding state (IB)

Release date: Nov. 23, 2016
Resolution: 5.3 Å
EM Method: Single-particle
Fitted PDBs: 5lzb
Q-score: 0.22

Fischer N, Neumann P, Bock LV, Maracci C, Wang Z, Paleskava A, Konevega AL, Schroder GF, Grubmuller H, Ficner R, Rodnina MV, Stark H

Nature (2016) 540 pp. 80-85 [ DOI: doi:10.1038/nature20560 Pubmed: 27842381 ]

Sample:

Water (18 Da, Ligand)
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli)
Initial binding state of e. coli ribosome 70s-selb-gdpnp-sec-trnasec-fmet-trnafmet-secis mrna complex (ib) (2 MDa, Complex from Escherichia coli)
Fmet-trnafmet (24 kDa, RNA from Escherichia coli)
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli)
Cca 3' end of e-site trnasec (low occupancy) (894 Da, RNA from Escherichia coli)
N-formylmethionine (177 Da, Ligand)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli)
Selenocysteine (168 Da, Ligand)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
16s ribosomal rna (498 kDa, RNA from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
Secis mrna (15 kDa, RNA from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
Selenocysteine-specific elongation factor (68 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
Sec-trnasec (30 kDa, RNA from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)

[Show more]

EMD-4123

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Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the codon reading state (CR)

Release date: Nov. 23, 2016
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 5lzc
Q-score: 0.251

Fischer N, Neumann P, Bock LV, Maracci C, Wang Z, Paleskava A, Konevega AL, Schroder GF, Grubmuller H, Ficner R, Rodnina MV, Stark H

Nature (2016) 540 pp. 80-85 [ DOI: doi:10.1038/nature20560 Pubmed: 27842381 ]

Sample:

Water (18 Da, Ligand)
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
Codon reading state of e. coli ribosome 70s-selb-gdpnp-sec-trnasec-fmet-trnafmet-secis mrna complex (cr) (2 MDa, Complex from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli)
Fmet-trnafmet (24 kDa, RNA from Escherichia coli)
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli)
Cca 3' end of e-site trnasec (low occupancy) (894 Da, RNA from Escherichia coli)
N-formylmethionine (177 Da, Ligand)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli)
Selenocysteine (168 Da, Ligand)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
16s ribosomal rna (498 kDa, RNA from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
Secis mrna (15 kDa, RNA from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
Selenocysteine-specific elongation factor (68 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
Sec-trnasec (30 kDa, RNA from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)

[Show more]

EMD-4124

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Structure of SelB-Sec-tRNASec bound to the 70S ribosome in the GTPase activated state (GA)

Release date: Nov. 23, 2016
Resolution: 3.4 Å
EM Method: Single-particle
Fitted PDBs: 5lzd
Q-score: 0.452

Fischer N, Neumann P, Bock LV, Maracci C, Wang Z, Paleskava A, Konevega AL, Schroder GF, Grubmuller H, Ficner R, Rodnina MV, Stark H

Nature (2016) 540 pp. 80-85 [ DOI: doi:10.1038/nature20560 Pubmed: 27842381 ]

Sample:

Water (18 Da, Ligand)
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
Magnesium ion (24 Da, Ligand)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
Chloride ion (35 Da, Ligand)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli)
Fmet-trnafmet (24 kDa, RNA from Escherichia coli)
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli)
Cca 3' end of e-site trnasec (low occupancy) (894 Da, RNA from Escherichia coli)
N-formylmethionine (177 Da, Ligand)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli)
Selenocysteine (168 Da, Ligand)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
16s ribosomal rna (498 kDa, RNA from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
Secis mrna (15 kDa, RNA from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
Selenocysteine-specific elongation factor (68 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
Gtpase activated state of e. coli ribosome 70s-selb-gdpnp-sec-trnasec-fmet-trnafmet-secis mrna complex (ga) (2 MDa, Complex from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
Sec-trnasec (30 kDa, RNA from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)

[Show more]

EMD-4125

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Structure of the 70S ribosome with Sec-tRNASec in the classical pre-translocation state (C)

Release date: Nov. 23, 2016
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 5lze
Q-score: 0.431

Fischer N, Neumann P, Bock LV, Maracci C, Wang Z, Paleskava A, Konevega AL, Schroder GF, Grubmuller H, Ficner R, Rodnina MV, Stark H

Nature (2016) 540 pp. 80-85 [ DOI: doi:10.1038/nature20560 Pubmed: 27842381 ]

Sample:

30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
Classical pre-translocation state of e. coli ribosome 70s-sec-trnasec-fmet-trnafmet-secis mrna complex (c) (2 MDa, Complex from Escherichia coli)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli)
Fmetsec-trnasec (30 kDa, RNA from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli)
Cca 3' end of e-site trnasec (low occupancy) (894 Da, RNA from Escherichia coli)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
16s ribosomal rna (498 kDa, RNA from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
Secis mrna (15 kDa, RNA from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
Trnafmet (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)

[Show more]

EMD-4126

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Structure of the 70S ribosome with fMetSec-tRNASec in the hybrid pre-translocation state (H)

Release date: Nov. 23, 2016
Resolution: 4.6 Å
EM Method: Single-particle
Fitted PDBs: 5lzf
Q-score: 0.279

Fischer N, Neumann P, Bock LV, Maracci C, Wang Z, Paleskava A, Konevega AL, Schroder GF, Grubmuller H, Ficner R, Rodnina MV, Stark H

Nature (2016) 540 pp. 80-85 [ DOI: doi:10.1038/nature20560 Pubmed: 27842381 ]

Sample:

Hybrid pre-translocation state of e. coli ribosome 70s-fmetsec-trnasec-trnafmet-secis mrna complex (h) (2 MDa, Complex from Escherichia coli)
30s ribosomal protein s13 (12 kDa, Protein from Escherichia coli)
50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli)
50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
30s ribosomal protein s3 (23 kDa, Protein from Escherichia coli)
50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s6 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l33 (5 kDa, Protein from Escherichia coli)
30s ribosomal protein s18 (7 kDa, Protein from Escherichia coli)
Fmetsec-trnasec (30 kDa, RNA from Escherichia coli)
50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s2 (24 kDa, Protein from Escherichia coli)
50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli)
50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
30s ribosomal protein s5 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s7 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
16s ribosomal rna (498 kDa, RNA from Escherichia coli)
50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli)
50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
Secis mrna (15 kDa, RNA from Escherichia coli)
23s ribosomal rna (941 kDa, RNA from Escherichia coli)
50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
Zinc ion (65 Da, Ligand)
50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli)
30s ribosomal protein s11 (12 kDa, Protein from Escherichia coli)
30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
30s ribosomal protein s19 (9 kDa, Protein from Escherichia coli)
Trnafmet (24 kDa, RNA from Escherichia coli)
50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
5s ribosomal rna (38 kDa, RNA from Escherichia coli)
50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
30s ribosomal protein s21 (7 kDa, Protein from Escherichia coli)
50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)

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