Staphylococcus aureus 30S ribosomal subunit in presence of spermidine (body only)
Resolution: 3.2 Å
EM Method: Single-particle
Fitted PDBs: 7bgd
Q-score: 0.507
Belinite M, Khusainov I, Soufari H, Marzi S, Romby P, Yusupov M, Hashem Y
Front Mol Biosci (2021) 8 pp. 1022- [ ]
- 30s ribosomal protein s5 (17 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s18 (9 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s20 (9 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s16 (10 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- Staphylococcus aureus 30s ribosomal subunit in the presence of spermidine (body only) (Complex)
- Mrna (10 kDa, RNA from Staphylococcus aureus)
- 30s ribosomal protein s4 (23 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s15 (10 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s17 (10 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 16s ribosomal rna (503 kDa, RNA from Staphylococcus aureus)
- 30s ribosomal protein s6 (11 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal subunit (Complex from Staphylococcus aureus subsp. aureus NCTC 8325)
- Mrna (Complex from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s8 (14 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s12 (15 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s11 (13 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s2 (29 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
Staphylococcus aureus 30S ribosomal subunit in presence of spermidine (head only)
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7bge
Q-score: 0.432
Belinite M, Khusainov I, Soufari H, Marzi S, Romby P, Yusupov M, Hashem Y
Front Mol Biosci (2021) 8 pp. 1022- [ ]
- 16s ribosomal rna (503 kDa, RNA from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s3 (24 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s10 (11 kDa, Protein from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s19 (10 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s7 (17 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s9 (14 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s13 (13 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- Staphylococcus aureus 30s ribosomal subunit in presence of spermidine (head only) (Complex from Staphylococcus aureus subsp. aureus NCTC 8325)
- 30s ribosomal protein s14 type z (7 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
- 30s ribosomal protein s2 (29 kDa, Protein from Staphylococcus aureus (strain NCTC 8325))
Bacterial 30S ribosomal subunit assembly complex state M (Consensus refinement)
Resolution: 2.9 Å
EM Method: Single-particle
Fitted PDBs: 7boe
Q-score: 0.391
Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf7547 Pubmed: 34088665 ]
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
- 16s rrna (499 kDa, RNA from Escherichia coli (strain K12))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
- Complete bacterial 30s ribosomal subunit assembly complex state m (consensus refinement) (Complex from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
Complete Bacterial 30S ribosomal subunit assembly complex state E (+RbfA)(Consensus Refinement)
Resolution: 2.82 Å
EM Method: Single-particle
Fitted PDBs: 7boh
Q-score: 0.442
Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf7547 Pubmed: 34088665 ]
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
- Complete bacterial 30s ribosomal subunit assembly (Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosome-binding factor (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosome-binding factor (Complex from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Complete bacterial 30s ribosomal subunit assembly complex state e (+rbfa) (consensus refinement) (Complex)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
- Rna (1527-mer) (499 kDa, RNA from Escherichia coli (strain K12))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
Complete Bacterial 30S ribosomal subunit assembly complex state F (+RsgA)(Consensus Refinement)
Resolution: 3.0 Å
EM Method: Single-particle
Fitted PDBs: 7nar
Q-score: 0.435
Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf7547 Pubmed: 34088665 ]
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
- Bacterial 30s ribosomal subunit assembly (Complex from Escherichia coli (strain K12))
- Small ribosomal subunit biogenesis gtpase rsga (39 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
- 16s rrna (499 kDa, RNA from Escherichia coli (strain K12))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
- Complete bacterial 30s ribosomal subunit assembly complex state f (+rsga)(consensus refinement) (Complex)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli (strain K12))
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
- Small ribosomal subunit biogenesis gtpase rsga (Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
Bacterial 30S ribosomal subunit assembly complex state A (Consensus refinement)
Resolution: 3.59 Å
EM Method: Single-particle
Fitted PDBs: 7nat
Q-score: 0.345
Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf7547 Pubmed: 34088665 ]
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
- Bacterial 30s ribosomal subunit assembly (Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
- 16s rrna (499 kDa, RNA from Escherichia coli (strain K12))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
- Bacterial 30s ribosomal subunit assembly complex state a (consensus refinement) (Complex)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s21 (8 kDa, Protein from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
- Ribosome maturation factor rimp (Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
- Ribosome maturation factor rimp (16 kDa, Protein from Escherichia coli (strain K12))
Bacterial 30S ribosomal subunit assembly complex state C (Consensus Refinement)
Resolution: 3.78 Å
EM Method: Single-particle
Fitted PDBs: 7nau
Q-score: 0.331
Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf7547 Pubmed: 34088665 ]
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
- 16s rrna (499 kDa, RNA from Escherichia coli (strain K12))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Bacterial 30s ribosomal assembly (Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
- Bacterial 30s ribosomal subunit assembly complex state c (consensus refinement) (Complex)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
- Ribosome maturation factor rimp (Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
- Ribosome maturation factor rimp (16 kDa, Protein from Escherichia coli (strain K12))
Bacterial 30S ribosomal subunit assembly complex state D (Consensus refinement)
Resolution: 4.8 Å
EM Method: Single-particle
Fitted PDBs: 7nav
Q-score: 0.278
Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf7547 Pubmed: 34088665 ]
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli (strain K12))
- Bacterial 30s ribosomal subunit assembly (Complex from Escherichia coli (strain K12))
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli (strain K12))
- 16s rrna (499 kDa, RNA from Escherichia coli (strain K12))
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosome-binding factor (15 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosome-binding factor, ribosome maturation factor rimp (Complex from Escherichia coli (strain K12))
- Magnesium ion (24 Da, Ligand)
- Bacterial 30s ribosomal subunit assembly complex state d (consensus refinement) (Complex)
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli (strain K12))
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli (strain K12))
- Ribosome maturation factor rimp (16 kDa, Protein from Escherichia coli (strain K12))
Complete Bacterial 30S ribosomal subunit assembly complex state I (Consensus Refinement)
Resolution: 2.96 Å
EM Method: Single-particle
Fitted PDBs: 7nax
Q-score: 0.389
Schedlbauer A, Iturrioz I, Ochoa-Lizarralde B, Diercks T, Lopez-Alonso JP, Lavin JL, Kaminishi T, Capuni R, Dhimole N, de Astigarraga E, Gil-Carton D, Fucini P, Connell SR
Sci Adv (2021) 7 [ DOI: doi:10.1126/sciadv.abf7547 Pubmed: 34088665 ]
- 30s ribosomal protein s10 (11 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s2 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s17 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s7 (20 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s5 (17 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s20 (9 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s6 (15 kDa, Protein from Escherichia coli)
- Complete bacterial 30s ribosomal subunit assembly complex state i (consensus refinement) (Complex from Escherichia coli)
- 30s ribosomal protein s12 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s19 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s11 (13 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 30s ribosomal protein s18 (9 kDa, Protein from Escherichia coli)
- 16s rrna (499 kDa, RNA from Escherichia coli)
- 30s ribosomal protein s9 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s4 (23 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s14 (11 kDa, Protein from Escherichia coli)
- Magnesium ion (24 Da, Ligand)
- 30s ribosomal protein s15 (10 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s13 (13 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s3 (26 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s8 (14 kDa, Protein from Escherichia coli)
- 30s ribosomal protein s16 (9 kDa, Protein from Escherichia coli)
Staphylococcus aureus 30S ribosomal subunit in absence of spermidine with fixed helix 44
Belinite M, Khusainov I, Soufari H, Marzi S, Romby P, Yusupov M, Hashem Y
Front Mol Biosci (2021) 8 pp. 738752-738752 [ DOI: doi:10.3389/fmolb.2021.738752 Pubmed: 34869582 ]