Electron Microscopy Data Bank

EMD-6125

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Negative stain random conical tilt reconstructions of E. coli ribosomal 30S subunit assembly intermediates

Release date: Oct. 22, 2014
Resolution: 65.0 Å
EM Method: Single-particle

Sashital DG, Greeman CA, Lyumkis D, Potter C, Carragher B, Williamson JR

eLife (2014) 3 [ Pubmed: 25313868 DOI: doi:10.7554/eLife.04491 ]

Sample:

30s assembly intermediate (500 kDa, Complex from Escherichia coli BW25113)
Group i 30s ribosomal subunit assembly intermediate from wild-type e. coli (500 kDa, Sample)

EMD-6126

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Negative stain random conical tilt reconstructions of E. coli ribosomal 30S subunit assembly intermediates

Release date: Oct. 22, 2014
Resolution: 68.0 Å
EM Method: Single-particle

Sashital DG, Greeman CA, Lyumkis D, Potter C, Carragher B, Williamson JR

eLife (2014) 3 [ Pubmed: 25313868 DOI: doi:10.7554/eLife.04491 ]

Sample:

Group ii 30s ribosomal subunit assembly intermediate from wild-type e. coli (800 kDa, Sample)
30s assembly intermediate (800 kDa, Complex from Escherichia coli BW25113)

EMD-6127

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Negative stain random conical tilt reconstructions of E. coli ribosomal 30S subunit assembly intermediates

Release date: Oct. 22, 2014
Resolution: 55.0 Å
EM Method: Single-particle

Sashital DG, Greeman CA, Lyumkis D, Potter C, Carragher B, Williamson JR

eLife (2014) 3 [ Pubmed: 25313868 DOI: doi:10.7554/eLife.04491 ]

Sample:

Group iii 30s ribosomal subunit assembly intermediate from wild-type e. coli (800 kDa, Sample)
30s assembly intermediate (800 kDa, Complex from Escherichia coli BW25113)

EMD-6128

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Negative stain random conical tilt reconstructions of E. coli ribosomal 30S subunit assembly intermediates

Release date: Oct. 22, 2014
Resolution: 51.0 Å
EM Method: Single-particle

Sashital DG, Greeman CA, Lyumkis D, Potter C, Carragher B, Williamson JR

eLife (2014) 3 [ Pubmed: 25313868 DOI: doi:10.7554/eLife.04491 ]

Sample:

30s assembly intermediate (820 kDa, Complex from Escherichia coli BW25113)
Group iv 30s ribosomal subunit assembly intermediate from wild-type e. coli (820 kDa, Sample)

EMD-6129

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Negative stain random conical tilt reconstructions of E. coli ribosomal 30S subunit assembly intermediates

Release date: Oct. 22, 2014
Resolution: 46.0 Å
EM Method: Single-particle

Sashital DG, Greeman CA, Lyumkis D, Potter C, Carragher B, Williamson JR

eLife (2014) 3 [ Pubmed: 25313868 DOI: doi:10.7554/eLife.04491 ]

Sample:

30s assembly intermediate (820 kDa, Complex from Escherichia coli BW25113)
Group v 30s ribosomal subunit assembly intermediate missing platform density from wild-type e. coli (820 kDa, Sample)

EMD-6130

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Negative stain random conical tilt reconstructions of E. coli ribosomal 30S subunit assembly intermediates

Release date: Oct. 22, 2014
Resolution: 62.0 Å
EM Method: Single-particle

Sashital DG, Greeman CA, Lyumkis D, Potter C, Carragher B, Williamson JR

eLife (2014) 3 [ Pubmed: 25313868 DOI: doi:10.7554/eLife.04491 ]

Sample:

30s assembly intermediate (820 kDa, Complex from Escherichia coli BW25113)
Group v 30s ribosomal subunit assembly intermediate missing s3 density from wild-type e. coli (820 kDa, Sample)

EMD-6131

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Negative stain random conical tilt reconstructions of E. coli ribosomal 30S subunit assembly intermediates

Release date: Oct. 22, 2014
Resolution: 44.0 Å
EM Method: Single-particle

Sashital DG, Greeman CA, Lyumkis D, Potter C, Carragher B, Williamson JR

eLife (2014) 3 [ Pubmed: 25313868 DOI: doi:10.7554/eLife.04491 ]

Sample:

30s assembly intermediate (850 kDa, Complex from Escherichia coli BW25113)
Group v mature 30s ribosomal subunit from wild-type e. coli (850 kDa, Sample)

EMD-6132

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Negative stain random conical tilt reconstructions of E. coli ribosomal 30S subunit assembly intermediates

Release date: Oct. 22, 2014
Resolution: 58.0 Å
EM Method: Single-particle

Sashital DG, Greeman CA, Lyumkis D, Potter C, Carragher B, Williamson JR

eLife (2014) 3 [ Pubmed: 25313868 DOI: doi:10.7554/eLife.04491 ]

Sample:

Group ii 30s assembly intermediate from e. coli rimp deletion strain (volume 1 of 10) (800 kDa, Sample)
30s assembly intermediate (800 kDa, Complex from Escherichia coli BW25113)

EMD-6133

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Negative stain random conical tilt reconstructions of E. coli ribosomal 30S subunit assembly intermediates

Release date: Oct. 22, 2014
Resolution: 61.0 Å
EM Method: Single-particle

Sashital DG, Greeman CA, Lyumkis D, Potter C, Carragher B, Williamson JR

eLife (2014) 3 [ Pubmed: 25313868 DOI: doi:10.7554/eLife.04491 ]

Sample:

Group ii 30s assembly intermediate from e. coli rimp deletion strain (volume 6 of 10) (800 kDa, Sample)
30s assembly intermediate (800 kDa, Complex from Escherichia coli BW25113)

EMD-6134

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Negative stain random conical tilt reconstructions of E. coli ribosomal 30S subunit assembly intermediates

Release date: Oct. 22, 2014
Resolution: 55.0 Å
EM Method: Single-particle

Sashital DG, Greeman CA, Lyumkis D, Potter C, Carragher B, Williamson JR

eLife (2014) 3 [ Pubmed: 25313868 DOI: doi:10.7554/eLife.04491 ]

Sample:

30s assembly intermediate (800 kDa, Complex from Escherichia coli BW25113)
Group ii 30s assembly intermediate from e. coli rimp deletion strain (volume 5 of 10) (800 kDa, Sample)

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