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Tissue biology and disease modelling

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sample_name:"30S ribosomal subunit" AND author_name:"Lazennec-schurdevin C"

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EMD-14579

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Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA,mRNA, aIF1A and the C-terminal domain of aIF5B.

Release date: Aug. 3, 2022
Resolution: 2.77 Å
EM Method: Single-particle
Fitted PDBs: 7zag
Q-score: 0.56

Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E

Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]

Sample:

Met-trnaimet, translation initiation factor 1a and translation initiation factor 5b (Complex from Pyrococcus abyssi GE5)
Zn-ribbon rna-binding protein involved in translation (7 kDa, Protein from Pyrococcus abyssi GE5)
Methionine (149 Da, Ligand)
Translation initiation factor 5b (69 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s8e (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s19 (15 kDa, Protein from Pyrococcus abyssi GE5)
Translation initiation factor 1a (15 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s27e (7 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s14 type z (6 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s10 (11 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s7 (24 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s24e (11 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s5 (26 kDa, Protein from Pyrococcus abyssi GE5)
Rrna (487 kDa, RNA from Pyrococcus abyssi GE5)
30s and 50s ribosomal proteins (Complex from Pyrococcus abyssi GE5)
30s ribosomal protein s28e (8 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s27ae (5 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s17 (13 kDa, Protein from Pyrococcus abyssi GE5)
Mrna (8 kDa, RNA from Pyrococcus abyssi GE5)
Zinc ion (65 Da, Ligand)
Mrna (Complex from Pyrococcus abyssi GE5)
30s ribosomal protein s2 (23 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s6e (14 kDa, Protein from Pyrococcus abyssi GE5)
50s ribosomal protein l7ae (13 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s12 (16 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s8 (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s3ae (22 kDa, Protein from Pyrococcus abyssi GE5)
Complex of 30s ribosomal subunit with initiation factor aif1a, the c-terminal domain of aif5b, mrna and trna (Complex)
30s ribosomal protein s17e (8 kDa, Protein from Pyrococcus abyssi GE5)
Water (18 Da, Ligand)
30s ribosomal protein s3 (23 kDa, Protein from Pyrococcus abyssi GE5)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s9 (15 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s4 (21 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s11 (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s13 (16 kDa, Protein from Pyrococcus abyssi GE5)
50s ribosomal protein l41e (4 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s19e (17 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s4e (28 kDa, Protein from Pyrococcus abyssi GE5)
Met-trnaimet (24 kDa, RNA from Pyrococcus abyssi GE5)
30s ribosomal protein s15 (18 kDa, Protein from Pyrococcus abyssi GE5)

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EMD-14580

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Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA, mRNA, aIF1A and aIF5B

Release date: Aug. 3, 2022
Resolution: 2.7 Å
EM Method: Single-particle
Fitted PDBs: 7zah
Q-score: 0.591

Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E

Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]

Sample:

Zn-ribbon rna-binding protein involved in translation (7 kDa, Protein from Pyrococcus abyssi GE5)
Methionine (149 Da, Ligand)
Mrna (7 kDa, RNA from Pyrococcus abyssi GE5)
Translation initiation factor 5b (69 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s8e (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s19 (15 kDa, Protein from Pyrococcus abyssi GE5)
Translation initiation factor 1a (15 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s27e (7 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s14 type z (6 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s28e (7 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s10 (11 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s7 (24 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s24e (11 kDa, Protein from Pyrococcus abyssi GE5)
Initiation complex with 30s ribosomal subunit,trna, mrna and initiation factors 1a and 5b (Complex)
30s ribosomal protein s5 (26 kDa, Protein from Pyrococcus abyssi GE5)
Trna-met, translation initiation factor 1a and translation initiation factor 5b (Complex from Pyrococcus abyssi GE5)
30s and 50s ribosomal proteins (Complex from Pyrococcus abyssi GE5)
30s ribosomal protein s27ae (5 kDa, Protein from Pyrococcus abyssi GE5)
Trna-met (24 kDa, RNA from Pyrococcus abyssi GE5)
30s ribosomal protein s17 (13 kDa, Protein from Pyrococcus abyssi GE5)
Zinc ion (65 Da, Ligand)
Mrna (Complex from Pyrococcus abyssi GE5)
30s ribosomal protein s2 (23 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s6e (14 kDa, Protein from Pyrococcus abyssi GE5)
50s ribosomal protein l7ae (13 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s12 (16 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s8 (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s3ae (22 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s17e (8 kDa, Protein from Pyrococcus abyssi GE5)
Water (18 Da, Ligand)
30s ribosomal protein s3 (23 kDa, Protein from Pyrococcus abyssi GE5)
Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s9 (15 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s4 (21 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s11 (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s13 (16 kDa, Protein from Pyrococcus abyssi GE5)
50s ribosomal protein l41e (4 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s19e (17 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s4e (28 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s15 (18 kDa, Protein from Pyrococcus abyssi GE5)
16s rrna (487 kDa, RNA from Pyrococcus abyssi GE5)

[Show more]

EMD-14763

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Cryo-EM structure of aIF1A:aIF5B:Met-tRNAiMet complex from a Pyrococcus abyssi 30S initiation complex

Release date: July 20, 2022
Resolution: 3.6 Å
EM Method: Single-particle
Fitted PDBs: 7zki
Q-score: 0.418

Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E

Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]

Sample:

Translation initiation factor 1a and probable translation initiation factor if-2 (Complex from Pyrococcus abyssi GE5)
Trna-met (Complex from Escherichia coli)
Methionine (149 Da, Ligand)
Translation initiation factor 5b (69 kDa, Protein from Pyrococcus abyssi GE5)
Met-trnaimet (24 kDa, RNA from Escherichia coli)
Magnesium ion (24 Da, Ligand)
Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
Translation initiation factor 1a (15 kDa, Protein from Pyrococcus abyssi GE5)
Trna-aif5b-aif1a moiety of a pyrococcus abyssi translation initiation complex with 30s ribosomal subunit,trna, mrna and initiation factors 1a and 5b. (Complex)

[Show more]

EMD-14581

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Cryo-EM structure of a Pyrococcus abyssi 30S bound to Met-initiator tRNA, mRNA and aIF1A.

Release date: July 13, 2022
Resolution: 2.6 Å
EM Method: Single-particle
Fitted PDBs: 7zai
Q-score: 0.587

Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E

Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]

Sample:

Zn-ribbon rna-binding protein involved in translation (7 kDa, Protein from Pyrococcus abyssi GE5)
Methionine (149 Da, Ligand)
30s ribosomal protein s8e (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s19 (15 kDa, Protein from Pyrococcus abyssi GE5)
Translation initiation factor 1a (15 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s14 type z (6 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s10 (11 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s7 (24 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal subunit with initiation factor 1a, mrna and initiator trna (Complex from Pyrococcus abyssi GE5)
30s ribosomal protein s24e (11 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s5 (26 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s28e (8 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s27ae (5 kDa, Protein from Pyrococcus abyssi GE5)
Trna-met (24 kDa, RNA from Pyrococcus abyssi GE5)
30s ribosomal protein s17 (13 kDa, Protein from Pyrococcus abyssi GE5)
Mrna (8 kDa, RNA from Pyrococcus abyssi GE5)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s2 (23 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s6e (14 kDa, Protein from Pyrococcus abyssi GE5)
50s ribosomal protein l7ae (13 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s12 (16 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s8 (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s3ae (22 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s17e (8 kDa, Protein from Pyrococcus abyssi GE5)
Water (18 Da, Ligand)
30s ribosomal protein s3 (23 kDa, Protein from Pyrococcus abyssi GE5)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s9 (15 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s4 (21 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s11 (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s13 (16 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s27e (7 kDa, Protein from Pyrococcus abyssi)
50s ribosomal protein l41e (4 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s19e (17 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s4e (28 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s15 (18 kDa, Protein from Pyrococcus abyssi GE5)
16s rrna (487 kDa, RNA from Pyrococcus abyssi GE5)

[Show more]

EMD-14731

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High-resolution cryo-EM structure of Pyrococcus abyssi 30S ribosomal subunit bound to mRNA and initiator tRNA anticodon stem-loop

Release date: June 29, 2022
Resolution: 2.25 Å
EM Method: Single-particle
Fitted PDBs: 7zhg
Q-score: 0.696

Kazan R, Bourgeois G, Lazennec-Schurdevin C, Larquet E, Mechulam Y, Coureux PD, Schmitt E

Nucleic Acids Res (2022) 50 pp. 6532-6548 [ DOI: doi:10.1093/nar/gkac490 Pubmed: 35694843 ]

Sample:

Zn-ribbon rna-binding protein involved in translation (7 kDa, Protein from Pyrococcus abyssi GE5)
Met-trnaimet (Complex from Pyrococcus abyssi GE5)
High resolution 30s ribosomal subunit with a fragment of mrna and trna (Complex)
30s ribosomal protein s8e (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s19 (15 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s27e (7 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s14 type z (6 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s10 (11 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s7 (24 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s24e (11 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s5 (26 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s28e (8 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s27ae (5 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s17 (13 kDa, Protein from Pyrococcus abyssi GE5)
Zinc ion (65 Da, Ligand)
30s ribosomal protein s2 (23 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s6e (14 kDa, Protein from Pyrococcus abyssi GE5)
50s ribosomal protein l7ae (13 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s12 (16 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s8 (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s3ae (22 kDa, Protein from Pyrococcus abyssi GE5)
Mrna (5 kDa, RNA from Pyrococcus abyssi GE5)
30s ribosomal protein s17e (8 kDa, Protein from Pyrococcus abyssi GE5)
Water (18 Da, Ligand)
High resolution 30s ribosomal subunit with a fragment of mrna (Complex from Pyrococcus abyssi GE5)
30s ribosomal protein s3 (23 kDa, Protein from Pyrococcus abyssi GE5)
Magnesium ion (24 Da, Ligand)
30s ribosomal protein s9 (15 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s4 (21 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s11 (14 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s13 (16 kDa, Protein from Pyrococcus abyssi GE5)
50s ribosomal protein l41e (4 kDa, Protein from Pyrococcus abyssi GE5)
Met-trnaimet (24 kDa, RNA from Pyrococcus abyssi GE5)
30s ribosomal protein s19e (17 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s4e (28 kDa, Protein from Pyrococcus abyssi GE5)
30s ribosomal protein s15 (18 kDa, Protein from Pyrococcus abyssi GE5)
Rrna 16s (487 kDa, RNA from Pyrococcus abyssi GE5)

[Show more]

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Download

Select database:

EMDB [5]

Format:

Select the script type:

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