Escherichia coli 50S subunit in complex with the antimicrobial peptide Api137
Lauer SM, Reepmeyer M, Berendes O, Klepacki D, Gasse J, Gabrielli S, Grubmuller H, Bock LV, Krizsan A, Nikolay R, Spahn CMT, Hoffmann R
Nat Commun (2024) 15 pp. 3945-3945 [ Pubmed: 38730238 DOI: doi:10.1038/s41467-024-48027-4 ]
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
- Api137 (Complex from Apis mellifera)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- Large ribosomal subunit (Complex from Escherichia coli)
- Apidaecins type 137 (2 kDa, Protein from Apis mellifera)
- Large ribosomal subunit protein bl28 (8 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul15 (14 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
- Api137 in complex with the large ribosomal subunit (Complex)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation I
Lauer SM, Reepmeyer M, Berendes O, Klepacki D, Gasse J, Gabrielli S, Grubmuller H, Bock LV, Krizsan A, Nikolay R, Spahn CMT, Hoffmann R
Nat Commun (2024) 15 pp. 3945-3945 [ Pubmed: 38730238 DOI: doi:10.1038/s41467-024-48027-4 ]
- Api88 in complex with the large ribosomal subunit - conformation i (Complex)
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- Large ribosomal subunit (Complex from Escherichia coli)
- Large ribosomal subunit protein bl28 (8 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
- Apidaecins type 88 (2 kDa, Protein from Apis mellifera)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul15 (14 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
- Api88 (Complex from Apis mellifera)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation II
Resolution: 2.44 Å
EM Method: Single-particle
Fitted PDBs: 8rq0
Q-score: 0.557
Lauer SM, Reepmeyer M, Berendes O, Klepacki D, Gasse J, Gabrielli S, Grubmuller H, Bock LV, Krizsan A, Nikolay R, Spahn CMT, Hoffmann R
Nat Commun (2024) 15 pp. 3945-3945 [ Pubmed: 38730238 DOI: doi:10.1038/s41467-024-48027-4 ]
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- Large ribosomal subunit (Complex from Escherichia coli)
- Large ribosomal subunit protein bl28 (8 kDa, Protein from Escherichia coli)
- Api88 in complex with the large ribosomal subunit - conformation ii (Complex)
- Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
- Apidaecins type 88 (2 kDa, Protein from Apis mellifera)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul15 (14 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
- Api88 (Complex from Apis mellifera)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
Escherichia coli 50S subunit in complex with the antimicrobial peptide Api88 - conformation III
Lauer SM, Reepmeyer M, Berendes O, Klepacki D, Gasse J, Gabrielli S, Grubmuller H, Bock LV, Krizsan A, Nikolay R, Spahn CMT, Hoffmann R
Nat Commun (2024) 15 pp. 3945-3945 [ Pubmed: 38730238 DOI: doi:10.1038/s41467-024-48027-4 ]
- Large ribosomal subunit protein ul23 (10 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul29 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl31 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul22 (12 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul13 (16 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul2 (29 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul5 (20 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl35 (7 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl33 (5 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul16 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl32 (6 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- Api88 in complex with the large ribosomal subunit - conformation iii (Complex)
- Large ribosomal subunit (Complex from Escherichia coli)
- Large ribosomal subunit protein bl28 (8 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul18 (12 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl17 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl36a (4 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul4 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul6 (18 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul14 (13 kDa, Protein from Escherichia coli)
- Apidaecins type 88 (2 kDa, Protein from Apis mellifera)
- Large ribosomal subunit protein ul3 (22 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl20 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul15 (14 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul24 (11 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl21 (11 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- Large ribosomal subunit protein bl9 (15 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein ul30 (6 kDa, Protein from Escherichia coli)
- Api88 (Complex from Apis mellifera)
- Large ribosomal subunit protein bl27 (8 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl19 (13 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl34 (5 kDa, Protein from Escherichia coli)
- Large ribosomal subunit protein bl25 (10 kDa, Protein from Escherichia coli)
Resolution: 3.7 Å
EM Method: Single-particle
Fitted PDBs: 7bl2
Q-score: 0.328
Nikolay R, Hilal T, Schmidt S, Qin B, Schwefel D, Vieira-Vieira CH, Mielke T, Burger J, Loerke J, Amikura K, Flugel T, Ueda T, Selbach M, Deuerling E, Spahn CMT
Mol Cell (2021) 81 pp. 1200-1215.e9 [ Pubmed: 33639093 DOI: doi:10.1016/j.molcel.2021.02.006 ]
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Gtpase obge/cgta (43 kDa, Protein from Escherichia coli (strain K12))
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli (strain K12))
- Ribosomal silencing factor rsfs (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
- 23s ribosomal rna (946 kDa, RNA from Escherichia coli (strain K12))
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
- Assembly intermediate complex 1 (1 MDa, Complex from Escherichia coli (strain K12))
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
Resolution: 3.5 Å
EM Method: Single-particle
Fitted PDBs: 7bl3
Q-score: 0.362
Nikolay R, Hilal T, Schmidt S, Qin B, Schwefel D, Vieira-Vieira CH, Mielke T, Burger J, Loerke J, Amikura K, Flugel T, Ueda T, Selbach M, Deuerling E, Spahn CMT
Mol Cell (2021) 81 pp. 1200-1215.e9 [ Pubmed: 33639093 DOI: doi:10.1016/j.molcel.2021.02.006 ]
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli (strain K12))
- Ribosomal pre-50s assembly intermediate state 2 (1 MDa, Complex from Escherichia coli (strain K12))
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli (strain K12))
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Gtpase obge/cgta (43 kDa, Protein from Escherichia coli (strain K12))
- Ribosomal silencing factor rsfs (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
- Water (18 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
- 23s ribosomal rna (946 kDa, RNA from Escherichia coli (strain K12))
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
in vitro reconstituted 50S-ObgE-GMPPNP-RsfS particle
Resolution: 2.4 Å
EM Method: Single-particle
Fitted PDBs: 7bl4
Q-score: 0.492
Nikolay R, Hilal T, Schmidt S, Qin B, Schwefel D, Vieira-Vieira CH, Mielke T, Burger J, Loerke J, Amikura K, Flugel T, Ueda T, Selbach M, Deuerling E, Spahn CMT
Mol Cell (2021) 81 pp. 1200-1215.e9 [ Pubmed: 33639093 DOI: doi:10.1016/j.molcel.2021.02.006 ]
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli (strain K12))
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli (strain K12))
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli (strain K12))
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli (strain K12))
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli (strain K12))
- Zinc ion (65 Da, Ligand)
- Gtpase obge/cgta (43 kDa, Protein from Escherichia coli (strain K12))
- Ribosomal silencing factor rsfs (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli (strain K12))
- Water (18 Da, Ligand)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli (strain K12))
- In vitro reconstituted 50s-rsfs-obge-gmppnp complex (1 MDa, Complex from Escherichia coli (strain K12))
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli (strain K12))
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli (strain K12))
Resolution: 3.3 Å
EM Method: Single-particle
Fitted PDBs: 7bl5
Q-score: 0.363
Nikolay R, Hilal T, Schmidt S, Qin B, Schwefel D, Vieira-Vieira CH, Mielke T, Burger J, Loerke J, Amikura K, Flugel T, Ueda T, Selbach M, Deuerling E, Spahn CMT
Mol Cell (2021) 81 pp. 1200-1215.e9 [ Pubmed: 33639093 DOI: doi:10.1016/j.molcel.2021.02.006 ]
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l11 (14 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Guanosine-5'-diphosphate (443 Da, Ligand)
- Ribosomal silencing factor rsfs (11 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l10 (17 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Upf0307 protein yjga (21 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l17 (14 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Assembly intermediate complex 3 (1 MDa, Complex from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Gtpase obge/cgta (43 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Water (18 Da, Ligand)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Magnesium ion (24 Da, Ligand)
- 23s ribosomal rna (946 kDa, RNA from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- Ribosomal large subunit pseudouridine synthase d (37 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli str. K-12 substr. MG1655)
Resolution: 4.0 Å
EM Method: Single-particle
Fitted PDBs: 7bl6
Q-score: 0.289
Nikolay R, Hilal T, Schmidt S, Qin B, Schwefel D, Vieira-Vieira CH, Mielke T, Burger J, Loerke J, Amikura K, Flugel T, Ueda T, Selbach M, Deuerling E, Spahn CMT
Mol Cell (2021) 81 pp. 1200-1215.e9 [ Pubmed: 33639093 DOI: doi:10.1016/j.molcel.2021.02.006 ]
- In vitro reconstituted 50s-obge-gmppnp (1 MDa, Complex from Escherichia coli)
- 50s ribosomal protein l23 (11 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l27 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l16 (15 kDa, Protein from Escherichia coli)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli)
- 50s ribosomal protein l28 (9 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli)
- Gtpase obge/cgta (43 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l9 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l33 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l31 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli)
- Zinc ion (65 Da, Ligand)
- 50s ribosomal protein l35 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli)
- Water (18 Da, Ligand)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli)
- Phosphoaminophosphonic acid-guanylate ester (522 Da, Ligand)
- Magnesium ion (24 Da, Ligand)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l36 (4 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l15 (15 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli)
50S ribosomal subunit assembly intermediate state 3
Resolution: 4.3 Å
EM Method: Single-particle
Fitted PDBs: 6gc4
Q-score: 0.262
Nikolay R, Hilal T, Qin B, Mielke T, Burger J, Loerke J, Textoris-Taube K, Nierhaus KH, Spahn CMT
Mol Cell (2018) 70 pp. 881-893.e3 [ DOI: doi:10.1016/j.molcel.2018.05.003 Pubmed: 29883607 ]
- 50s ribosomal assembly intermediate state 3 (Complex from Escherichia coli K-12)
- 50s ribosomal protein l34 (5 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l13 (16 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l21 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l22 (12 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l28 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l4 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l27 (8 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l3 (22 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l23 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l5 (20 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l15 (14 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l19 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l20 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l14 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l18 (12 kDa, Protein from Escherichia coli K-12)
- 23s ribosomal rna (941 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l6 (18 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l32 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l17 (13 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l2 (29 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l30 (6 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l9 (5 kDa, Protein from Escherichia coli K-12)
- 5s ribosomal rna (38 kDa, RNA from Escherichia coli K-12)
- 50s ribosomal protein l24 (11 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l25 (10 kDa, Protein from Escherichia coli K-12)
- 50s ribosomal protein l29 (7 kDa, Protein from Escherichia coli K-12)